geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

The domain organization of p67 phox, a protein required for activation of the superoxide-producing NADPH oxidase in phagocytes


, : The domain organization of p67 phox, a protein required for activation of the superoxide-producing NADPH oxidase in phagocytes. Journal of Innate Immunity 1(6): 543-555

The phagocyte NADPH oxidase, crucial for innate immunity, is dormant in resting cells, but becomes activated during phagocytosis to produce superoxide, a precursor of microbicidal oxidants. In activation of the oxidase, the multidomain protein p67(phox)plays a central role: it translocates to the membrane as a ternary complex with p47(phox)and p40(phox), and interacts with the small GTPase Rac to assemble with the membrane-integrated catalytic protein gp91(phox), leading to superoxide production. Here we show, using small-angle X-ray scattering (SAXS) analysis, that p67(phox)adopts an elongated conformation when it exists not only as a monomer but also as the heterotrimer. Although p67(phox)harbors an N-terminal TPR domain for binding to Rac and a p40(phox)-interacting PB1 domain, followed by an SH3 domain that associates with p47(phox), the present model suggests that no or few apparent associations occur between the domains. The positions of the protein-interaction domains in p67(phox)contribute to activation of the phagocyte NADPH oxidase: the first SH3 domain that is located between the TPR and PB1 domains positively regulates oxidase activation only when it is present at the correct position; the PB1 domain placed at this SH3 domain position inhibits the oxidase by interacting with p40(phox).

US$19.90

PMID: 20375610

DOI: 10.1159/000235656


Other references

Charvát, J.; Chlumský, J.; Vanecek, T.; Samková, D.; Tylová, R.; Kvapil, M., 2006: The comparison of clinical and echocardiographic changes in diabetics 2nd type and nondiabetics in patients with shortness of breath due to left ventricular failure. In our study we have examined 198 patients admitted to hospital for shortness of breath at rest due to left ventricle failure. We have divided the patients into two groups according to the presence of diabetes mellitus. We have excluded the patien...

Gong, Y.C.; Yu, H.Z.; Yang, M.Q.; Chen, Y., 1993: A brief introduction to the cotton variety Emian 17. Emian 17 (833-18) was produced in Xiaogan by crossing the hybrid cotton T31 as the female parent with the male parent Paulownia and crossing the resulting F1 hybrid with T15. It is a vigorous, mid-maturity variety with a growth period of 125 days....

Lee, S.Jae.; Yim, H.Joon.; Hwang, S.Gyu.; Seo, Y.Seok.; Kim, J.Hoon.; Yoon, E.L.; Lee, J.Min.; Kim, B.Hyun.; Park, S.Jong.; Park, Y.Min.; Kim, H.Soo.; Lee, S.Hwan.; Ahn, S.Hoon.; Lee, J.Il.; Lee, J.Woo.; Kim, I.Hee.; Kim, H.Soo.; Hong, S.Pyo., 2013: Treatment of lamivudine-resistant chronic hepatitis B infection: a multicenter retrospective study. To compare the efficacy of rescue therapies in lamivudine (LAM)-resistant chronic hepatitis B (CHB) infections including: (1) adefovir dipivoxil (ADV) monotherapy, (2) ADV plus LAM combination therapy and (3) entecavir (ETV) 1.0 mg monotherapy. Th...

Josefson, D., 2002: Drug companies face pressure on profits. Bmj 324(7329): 65-65

Khan, T.A.; Bianchi, C.; Araujo, E.G.; Ruel, M.; Voisine, P.; Sellke, F.W., 2003: Activation of pulmonary mitogen-activated protein kinases during cardiopulmonary bypass. Purpose: Cardiopulmonary bypass (CPB) produces an inflammatory response associated with pulmonary dysfunction. Mitogen-activated protein kinases (MAPK) have been shown to mediate pulmonary injury. We hypothesized that MAPK are activated during CPB...

Tegtmeier, W., 1959: On the acoustically optimal construction of the ear-fitting pieces of electronic hearing aids. Zeitschrift für Arztliche Fortbildung 53: 1309-1311

Babaev, A.G.; Kharin, N.G., 1991: Map of desertification of arid territories of Asia. (Concept). Criteria and scales for compiling desertification maps of the arid territories of Asia are discused.

Koga, T.; Hashimoto, S.; Sugio, K.; Yonemitsu, Y.; Nakashima, Y.; Yoshino, I.; Matsuo, Y.; Mojtahedzadeh, S.; Sugimachi, K.; Sueishi, K., 2002: Lung adenocarcinoma with bronchioloalveolar carcinoma component is frequently associated with foci of high-grade atypical adenomatous hyperplasia. We assessed the occurrence of atypical adenomatous hyperplasia (AAH) in whole lung lobes with primary cancer lesions. Following surgical resection, tissue specimens were sliced to a thickness of 4 mm (3,641 specimens from 61 cases; mean = 59.7 spe...

Tichy, H.; Hellwig, M.; Kallina, W., 2017: Revisiting Theories of Humidity Transduction: A Focus on Electrophysiological Data. Understanding the mechanism of humidity transduction calls for experimental data and a theory to interpret the data and design new experiments. A comprehensive theory of humidity transduction must start with agreement on what humidity parameters a...

Vanhorebeek, I.; Langouche, L.; Van den Berghe, G., 2007: Tight blood glucose control: what is the evidence?. In two large, randomized studies, maintenance of normoglycemia with intensive insulin therapy largely prevented morbidity and reduced mortality of critically ill patients. Recently, questions have been raised about the efficacy and safety of this...