geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

The effect of normoxia and hypoxia on a prostate (PC-3) CD44/CD41 cell side fraction


, : The effect of normoxia and hypoxia on a prostate (PC-3) CD44/CD41 cell side fraction. Anticancer Research 31(2): 487-494

It has been reported that human prostate-derived PC-3 cells that are CD44- and CD 41 (a2 B1)-positive are enriched in cancer stem cells. This study compared the effect of PC-3 cell proliferation under normoxia or hypoxia on the initial and subsequent expression of this doubly-labeled side-fraction. Despite the numerical advantage of attached normoxic cells, 48 h of culture under nitrogen, an environment containing minimal oxygen and CO(2) resulting in an elevated pH of the medium, was associated with a higher percentage, absolute and relative number of doubly-labeled (DL) hypoxic compared to normoxic cells. At 24 h, the reverse was found. When the pH was controlled with the use of 95% nitrogen and 5% carbon dioxide, the percentage and number of normoxic DL cells exceeded hypoxic ones at both 24 and 48 h. At 24 h, 2-deoxy-L-glucose or sodium arsenate reduced normoxic DL cell numbers more than hypoxic ones. The interplay between hypoxia, increased medium pH and the effect of inhibitors as they might influence therapy are considered.

US$29.90

PMID: 21378328


Other references

Takehashi, M.; Kanatsu-Shinohara, M.; Shinohara, T., 2010: Generation of genetically modified animals using spermatogonial stem cells. Spermatogonial stem cells (SSCs) provide the foundation for spermatogenesis, and are unique tissue-specific stem cells because of their ability to transmit genetic information to offspring. Generation of knockout mice using mouse SSCs became feasi...

Ivanov, Ts, 1974: The characteristics of honey obtained on feeding sugar to bees. The organoleptic properties and chemical composition of honey from sugar-fed bees were investigated in 1971 and 1972 by feeding colonies in spring, summer and autumn with sugar syrup in a series of concentrations and quantities. Dilute syrup fed i...

Gao, Z.; Shi, T.; Luo, X.; Zhang, Z.; Zhuang, W.; Wang, L., 2013: High-throughput sequencing of small RNAs and analysis of differentially expressed microRNAs associated with pistil development in Japanese apricot. MicroRNAs (miRNAs) are a class of endogenous, small, non-coding RNAs that regulategene expression by mediating gene silencing at transcriptional and post-transcriptional levelsin high plants. However, the diversity of miRNAs and their roles in flo...

Walker, W.P.; Bhatia, D.K., 2014: Automated ingestion detection for a health monitoring system. Obesity is a global epidemic that imposes a financial burden and increased risk for a myriad of chronic diseases. Presented here is an overview of a prototype automated ingestion detection (AID) process implemented in a health monitoring system (H...

Sajap, AS., 1988: Histopathology of transovarial transmission of Nosema pyrausta in the European corn borer, Ostrinia nubilalis. Neonate to 5th-instar larvae of Ostrinia nubilalis were exposed to 100, 200, 400 and 800 Nosema pyrausta spores/mmsuperscript 2 diet surface for 48 h. Infections were evident in the reproductive tissues in all insects 7 days after exposure, regard...

Dipietro, N.T.; Nicholson, D.A.; Muckler, A.S.; Rabinak, C.; Halverson, H.E.; Freeman, J.H., 2002: Eyeblink conditioned response timing in adult and infant rats. The peak amplitude of the eyeblink conditioned response (CR) is precisely timed, occurring at the onset time of the unconditioned stimulus. The precision of CR timing has been examined using conditioning procedures with more than one interstimulus...

Bittrich V.; Hartmann H.E.K., 1988: The aizoaceae a new approach. The history of the Aizoaceae Rudolphi in its various circumscriptions is reviewed, and the monophyly of the Aizoaceae sensu stricto after exclusion of Molluginaceae is demonstrated. A subdivision of the family and the relevant synapomorphic charac...

Stadnichenko, A.P., 1974: Infestation of Unio pictorum and Anodonta piscinalis (Mollusca, Lamellibranchia) by parthenitae of Bucephalus polymorphus Baer (Trematodes) and the action of the parasites on the hosts' organism. Parazitologiia 8(5): 420-425

Shkolnikov, V.; Bahga, S.S.; Santiago, J.G., 2012: Desalination and hydrogen, chlorine, and sodium hydroxide production via electrophoretic ion exchange and precipitation. We demonstrate and analyze a novel desalination method which works by electrophoretically replacing sodium and chloride in feed salt water with a pair of ions, calcium and carbonate, that react and precipitate out. The resulting calcium carbonate...

Laub M.C.; Alberti P., 1984: Ultrasound ventriculography a mode of the intra cerebral ventricles in new born relationship to maturation and clinical significance. Since during fetal development the intracerebral ventricular system shows characteristic changes, the verification of whether there is a relationship between the width of the ventricles of the newborn and the degree of maturity was attempted. In 3...