geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

The effect of valdecoxib on the production of growth factors evoked by hypoxia and bacterial lipopolysaccharide in HMEC-1 cells


, : The effect of valdecoxib on the production of growth factors evoked by hypoxia and bacterial lipopolysaccharide in HMEC-1 cells. Advances in Clinical and Experimental Medicine 22(6): 795-800

Endothelial cells produce prostaglandins (PGE2 and PGI2) and growth factors (VEGF and bFGF). These substances regulate proliferation of cells, inflammatory processes and neovascularization under physiological and pathological conditions. The aim of this study was to check whether valdecoxib - a selective COX-2 inhibitor - inhibits VEGF and/or bFGF secretion in the presence of LPS or cobalt chloride in normal human microvascular endothelial cells (HMEC-1). HMEC-1 cells were treated with valdecoxib at a concentration of 10 and 100 μM in the presence of 100 μg/mL LPS or 200 μM CoCl2. The effect of NSAIDs and LPS on VEGF and bFGF proteins was analyzed by ELISA kit (R&D Systems). Cell viability was measured using the 3-[4.5-dimethylthiazol-2-yl]-2.5-diphenyltetrazolium bromide (MTT) method. Valdecoxib inhibited LPS-induced proliferation of endothelial cells and bFGF secretion in a dose-dependent manner. Valdecoxib stimulated VEGF formation via HMEC-1 under inflammatory conditions.

US$29.90

PMID: 24431307


Other references

Thornton, C.A.; Griggs, R.C.; Moxley, R.T., 1994: Myotonic dystrophy with no trinucleotide repeat expansion. We report 3 patients from 2 families with myotonic dystrophy who do not show an abnormal expansion of CTG trinucleotide repeats within the myotonic dystrophy gene. Characteristic features of myotonic dystrophy in these patients were frontal baldin...

Belkina N.M.; Rybin A.I., 1974: On the book by Yu. A. Zorin Recent Structure and Isostasy of the Baykal Rift Zone and of the Neighboring Areas (Nauka Press, 1971). Physics of the Solid Earth 6: 409-413

Gregersen, M.I., 1957: The contribution of I 131-labeled proteins to measurements of blood volume: discussion. Annals of the New York Academy of Sciences 70(1): 147-149

Hayashi T.; Tsuda N.; Ayabe H.; Mochinaga N., 1991: Review of breast lesions with uncertain words or corrections in the histopathologic diagnosis. As is the case with other organs, breast lesions sometimes present difficulties in pathologic diagnosis. We examined 355 cases filled as breast lesions at the Pathology Division, Central Diagnostic Laboratory, Nagasaki University Hospital during t...

E.S.erbini, N.R.; Ibrahim, I.A.; E.S.nbaty, M.R.; Reda, A.A.; Emam, H.E., 2002: Effect of NAA and IBA concentration on growth, rooting and acclimatization of banana plantlet. Banana (Musa acuminata cv. William's) shoots were grown in vitro on half-strength Murashige and Skoog (MS) rooting medium supplemented with NAA+IBA (0, 0.5 or 1.0 mg/litre each). The cultures were incubated at 27+or-1 degrees C under a 16/8 h...

Suma H.; Asada K.; Sasaki S.; Takeuchi A.; Suwa M.; Hirota Y., 1986: Intraoperative evaluation of bypass graftability of proximally obstructed coronary artery by ultrasonic echography. Japanese Circulation Journal 50(6): 525

Kokatnur, L.; Rudrappa, M., 2015: Primary aorto-esophageal fistula: Great masquerader of esophageal variceal bleeding. Aorto-esophageal fistula is a rare cause of upper gastrointestinal bleeding. Thoracic aneurysm, the most common cause of this condition, will slowly increase over time and can erode the wall of the aorta creating a fistula and leading to torrentia...

Salum, I.; Borjeson, O., 1957: Bibliotherapy and its value in care of mentally ill patients. Social-Medicinsk Tidskrift 34(1): 7-12

Giani, J.F.; Fuchs, S.; Gonzalez-Villalobos, R.A., 2013: Angiotensin II type 1 receptor-associated protein: a novel modulator of angiotensin II actions in the nephron. Hypertension 61(6): 1150-1152

Rea T.H.; Quismorio F.; Nies K.; Harding B.; Disaia P.; Levan N.; Friou G., 1974: Intra dermal antigens epi cutaneous haptens thymus derived cell counts bone marrow derived cell counts lymphocyte transformation and auto antibodies in 1 group of patients with lepromatous leprosy. International Journal of Leprosy & Other Mycobacterial Diseases 42(3): 369-371