+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

The erythropoietin/erythropoietin receptor signaling pathway promotes growth and invasion abilities in human renal carcinoma cells

, : The erythropoietin/erythropoietin receptor signaling pathway promotes growth and invasion abilities in human renal carcinoma cells. Plos One 7(9): E45122-E45122

Co-expression of erythropoietin (Epo) and erythropoietin receptor (EpoR) has been found in various non-hematopoietic cancers including hereditary and sporadic renal cell carcinomas (RCC), but the Epo/EpoR autocrine and paracrine mechanisms in tumor progression have not yet been identified. In this study, we used RNA interference method to down-regulate EpoR to investigate the function of Epo/EpoR pathway in human RCC cells. Epo and EpoR co-expressed in primary renal cancer cells and 6 human RCC cell lines. EpoR signaling was constitutionally phosphorylated in primary renal cancer cells, 786-0 and Caki-1 cells, and recombinant human Epo (rhEpo) stimulation had no significant effects on further phosphorylation of EpoR pathway, proliferation, and invasiveness of the cells. Down-regulation of EpoR expression in 786-0 cells by lentivirus-introduced siRNA resulted in inhibition of growth and invasiveness in vitro and in vivo, and promotion of cell apoptosis. In addition, rhEpo stimulation slightly antagonized the anti-tumor effect of Sunitinib on 786-0 cells. Sunitinib could induce more apoptotic cells in 786-0 cells with knockdown EpoR expression. Our results suggested that Epo/EpoR pathway was involved in cell growth, invasion, survival, and sensitivity to the multi-kinases inhibitor Sunitinib in RCC cells.


PMID: 23028796

DOI: 10.1371/journal.pone.0045122

Other references

Yamada M.; Azuma M.; Nishioka R.; Togashi T., 1970: Relationship between immunity and interferon production in macrophages part 1 effect of immunity on interferon production. Japanese Journal of Microbiology 14(4): 311-318

Meyerhardt, J.A.; Heseltine, D.; Ogino, S.; Clark, J.W.; Enzinger, P.C.; Ryan, D.P.; Earle, C.C.; Zhu, A.X.; Fuchs, C.S., 2006: Efficacy of cetuximab after treatment with oral epidermal growth factor receptor tyrosine kinase inhibitor-based chemotherapy in metastatic colorectal cancer. We studied the efficacy of cetuximab therapy in patients with metastatic colorectal cancer (mCRC) previously treated with an oral inhibitor of the tyrosine kinase domain of the epidermal growth factor receptor. We reviewed the posttrial records of...

Beal, M.F.; Mazurek, M.F.; Ellison, D.W.; Kowall, N.W.; Solomon, P.R.; Pendlebury, W.W., 1989: Neurochemical characteristics of aluminum-induced neurofibrillary degeneration in rabbits. Aluminum-induced neurofibrillary degeneration in rabbits is known to affect particular populations of neurons. The neurotransmitter alterations which accompany aluminum neurofibrillary degeneration were examined in order to assess how closely they...

Alvarez M.; Granados R.; Rosell G.; Santalo P.; Salas M., 1984: Synthesis and pharmacological results of heterocyclic analogs of benextramine as alpha adrenergic blockers. The synthesis and the pharmacological results of the new tetramine disulfides are described. These compounds are irreversible blockers of the .alpha.-adrenoceptor, and all are structural analog of benextramine by substitution of the o-methoxybenzy...

Tapfer; Ploog, 1989: X-ray interference in ultrathin epitaxial layers: A versatile method for the structural analysis of single quantum wells and heterointerfaces. Physical Review. B, Condensed Matter 40(14): 9802-9810

Kovacs, K.; Yeghiayan, E.; Hatakeyama, S.; Selye, H., 1970: Adrenocortical lipoid hyperplasia induced in rats by aniline. Experientia 26(9): 1014-1015

Anand, RK., 1984: A note on the aberrations on the elytra of Mylabris phalerata Pallas Coleoptera, Meloidae. Uttar Pradesh Journal of Zoology, 42: 213-216

Strenth, N.E., 1976: A review of the systematics and zoo geography of the fresh water species of palaemonetes of north america crustacea decapoda. Three new species of freshwater Palaemonetes [P. holthuisi, P. texanus, P. mexicanus ] from Texas [USA] and Mexico are described. A key to the known North American species [P. antrorum, P. paludosus, P. kadiakensis, P. cummingi, P. suttkusi, P. li...

Poulsen, W.B.; Dove, S.B.; del Rio, C.E., 1995: Effect of nickel-titanium engine-driven instrument rotational speed on root canal morphology. Thirty-six mesial canals in 18 extracted human mandibular molars were randomly allocated to three experimental groups. Specimens were prepared and evaluated using a modified Bramante technique. Teeth were sectioned at the midroot and apical region...

Drees, O., 1958: Experimental contribution to the examination of disinfectants in poliomyelitis. II. Experiments on hygienic disinfection of the hands. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. 1. Abt. Medizinisch-Hygienische Bakteriologie, Virusforschung und Parasitologie. Originale 173(7-8): 539-550