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The interaction of zinc with membrane-associated 18.5 kDa myelin basic protein: an attenuated total reflectance-Fourier transform infrared spectroscopic study


, : The interaction of zinc with membrane-associated 18.5 kDa myelin basic protein: an attenuated total reflectance-Fourier transform infrared spectroscopic study. Amino Acids 39(3): 739-750

Myelin basic protein (MBP) is an essential structural protein required for tight compaction of the myelin sheath of the central nervous system, and belongs to the family of intrinsically disordered proteins. It contains a high proportion of polar and charged amino acids, and has an adaptive conformation depending on its environment and binding surfaces (membranes) or partners (other proteins or small ligands including divalent cations). Zinc is an important stabilizing component of myelin and its concentration is substantially higher than that of any other trace element in the brain. In this study, we investigate the effect of zinc on different variants of 18.5 kDa MBP, including new recombinant forms lacking hexahistidine tags which would interfere with the binding of the cation. Isothermal titration calorimetry showed the dissociation constant to be in the micromolar range for all variants. Circular dichroism spectroscopy showed that there was minimal effect of zinc on the secondary structure on MBP in aqueous solution. When MBP was reconstituted with myelin-mimetic membranes, attenuated total reflectance-Fourier transform infrared spectroscopy revealed that there was a rearrangement of secondary structure components upon addition of zinc that was subtly different for each variant, indicative of a synergistic protein-membrane-cation interaction.

US$19.90

PMID: 20169373

DOI: 10.1007/s00726-010-0513-7


Other references

Weisgerber G.; Heinrich G., 1983: Laurion, and no end? Critical remarks on exploration in a mining region which was very important in antiquity. Der Anschnitt 35(6): 190-200

Lie, K.J.; Heyneman, D., 1976: Studies on resistance in snails part 5 tissue reactions to echinostoma lindoense in naturally resistant biomphalaria glabrata. Laboratory-raised juvenile albino B. glabrata snails show a wide range of natural resistance to a single infection with 50 or 100 miracidia of E. lindoense. In the most resistant snails all sporocysts are destroyed in peripheral tissues soon after...

Rothschuh, K.E., 1959: Physiology between yesterday & tomorrow; a historical study on the relations of physiology to medicine with special reference to the 19th century. Hippokrates 30(1): 7-12

Meeder, P.J.; Holz, U., 1985: Replacement-plasty of the coronoid process in unstable elbow joint dislocations with avulsion fracture of the coronoid process and radius head crush fracture. Aktuelle Traumatologie 15(2): 89-90

Tamaki Kensaku; Yamamoto Kiyohiko, 1992: Estimating in-situ stress field from basaltic rock core samples of Hole 794C, Yamato Basin, Japan Sea. Proceedings of the Ocean Drilling Program, Scientific Results 127-128: 1047-1055

Moriuchi T.; Kasai M.; Kobayashi H., 1979: Serological characterization of rat leukemia lines dbla 1 dbla 6 and dbla 9. Three lines of rat leukemia, DBLA-1, -6 and -9, were studied serologically by the complement-dependent cytotoxicity test. DBLA-1, -6 and -9 were killed by anti-lymphocyte sera, anti-Thy-1.1 sera and rabbit anti-rat brain sera absorbed with AKR/J b...

Cavaleiro J.A.S.; Kenner G.W.; Smith K.M., 1973: Biosynthetic intermediates between copro porphyrinogen iii and proto porphyrin ix. Journal of the Chemical Society Chemical Communications (5): 183-184

Anonymous, 1992: Abstracts and Index of the 26th annual meeting of the European Society for Clinical Investigation. April 1-4, Vienna, Austria. Abstracts. European Journal of Clinical Investigation 22(4 Pt 2): A1-56

Costes, Y.; Garnier, D.; Bachour, K.; Flicoteaux, H.; Neidhardt, A., 1990: Repetitive inhalation anesthesia in rats. Inhalational anesthesia in the rat seems a good process, by a rapid induction and awakening if a steady state ventilation-perfusion ratio and no pollution are obtained. Animals are cooped up in an "induction-box" which is joined to a sca...

Martin, R.S.; Hayes, B.J.; Hutchinson, A.; Yates, P.; Lim, W.Kwang., 2017: Implementation of 'Goals of Patient Care' medical treatment orders in residential aged care facilities: protocol for a randomised controlled trial. Systematic reviews demonstrate that advance care planning (ACP) has many positive effects for residents of aged care facilities, including decreased hospitalisation. The proposed Residential Aged Care Facility (RACF) 'Goals of Patient Care&ap...