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The involvement of CD146 and its novel ligand Galectin-1 in apoptotic regulation of endothelial cells


, : The involvement of CD146 and its novel ligand Galectin-1 in apoptotic regulation of endothelial cells. Journal of Biological Chemistry 288(4): 2571-2579

CD146 is a highly glycosylated junctional adhesion molecule, expressed on human vascular endothelial cells and involved in the control of vessel integrity. Galectin-1 is a lectin produced by vascular cells that can binds N- and O-linked oligosaccharides of cell membrane glycoproteins. Because both CD146 and Galectin-1 are involved in modulation of cell apoptosis, we hypothesized that Galectin-1 could interact with CD146, leading to functional consequences in endothelial cell apoptosis. We first characterized CD146 glycosylations and showed that it is mainly composed of N-glycans able to establish interactions with Galectin-1. We demonstrated a sugar-dependent binding of recombinant CD146 to Galectin-1 using both ELISA and Biacore assays. This interaction is direct, with a K(D) of 3.10(-7) M, and specific as CD146 binds to Galectin-1 and not to Galectin-2. Moreover, co-immunoprecipitation experiments showed that Galectin-1 interacts with endogenous CD146 that is highly expressed by HUVEC. We observed a Galectin-1-induced HUVEC apoptosis in a dose-dependent manner as demonstrated by Annexin-V/7AAD staining. Interestingly, both down-regulation of CD146 cell surface expression using siRNA and antibody-mediated blockade of CD146 increase this apoptosis. Altogether, our results identify Galectin-1 as a novel ligand for CD146 and this interaction protects, in vitro, endothelial cells against apoptosis induced by Galectin-1.

US$19.90

PMID: 23223580

DOI: 10.1074/jbc.M112.418848


Other references

Beric B.; Kranz F.; Marjanovic M., 1983: Experiences with the use of prostin 15 m in termination of pregnancy in the 2nd trimester in young patients. Archives of Gynecology 235(1-4): 604-606

Yamamoto, T., 1975: Frequency of detection and survival of infectious pancreatic necrosis virus in a carrier population of brook trout (Salvelimus fontinalis) in a lake. Over 21/2 years the carrier rate of the virus among trout released in a small, enclosed lake decreased from 90% to 69%, though the infection rates of the different organs varied. The data indicate that carrier fish released into a lake do not read...

Iliew, D.; Georgiewa, S.; Siwtschew, S.; Skatschokowa, X., 1967: The Marfan syndrome (clinical, histopathological and genetic study of a case). Zeitschrift für die Gesamte Innere Medizin und Ihre Grenzgebiete 22(7): 219-223

Morgan, J.F.; Heuchert, M.D.; Kirk, H.D., 1969: The effect of storage at low temperatures on the mouse strain specificity of the 6C3HED ascites tumor. Cancer Research 29(4): 855-861

Warner, B.A.; DiPalma, J.A., 1994: Oral sodium phosphate catharsis: "first do no harm". American Journal of Gastroenterology 89(7): 1118-1119

Moczydlowski E.G.; Latorre R., 1983: Gating kinetics of a calcium activated potassium channel in planar lipid bi layers evidence for 2 voltage dependent calcium binding reactions. Biophysical Journal 41(2 PART 2): 56A

Schoenfeld, P.; Cook, D.; Hamilton, F.; Laine, L.; Morgan, D.; Peterson, W., 1998: An evidence-based approach to gastroenterology therapy. Gastroenterology 114(6): 1318-1325, June

Takahashi, T.; Wood, S.J.; Soulsby, B.; McGorry, P.D.; Tanino, R.; Suzuki, M.; Velakoulis, D.; Pantelis, C., 2009: Follow-up MRI study of the insular cortex in first-episode psychosis and chronic schizophrenia. Morphologic abnormalities of the insular cortex have been described in psychotic disorders such as schizophrenia, but it remains unknown whether these abnormalities develop progressively over the course of the illness. In the current study, longit...

Goldsmith, J., 1988: Ambulatory services help pull hospitals out of the red, but they still get no respect. Modern Healthcare 18(19): 36-36

Stalinski, Z.; Karlowicz, W.; Szarek, J., 1968: Preliminary analysis of the relationship between evaluation for show purposes and commercial evaluation of bulls. Data were analysed on 74 Polish Red bulls aged 12- 18 mth. Correlations and regression coefficients are tabulated. Evaluation for show purposes was based on pedigree (max. score, 5 points), milk yield of dam (5), fat content of milk (5), and healt...