geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

The nitrogen interaction network in Synechococcus WH5701, a cyanobacterium with two PipX and two P(II)-like proteins


, : The nitrogen interaction network in Synechococcus WH5701, a cyanobacterium with two PipX and two P(II)-like proteins. Microbiology 157(Pt 4): 1220-1228

Nitrogen regulation involves the formation of different types of protein complexes between signal transducers and their transcriptional or metabolic targets. In oxygenic phototrophs, the signal integrator P(II) activates the enzyme N-acetyl-l-glutamate kinase (NAGK) by complex formation. P(II) also interacts with PipX, a protein with a tudor-like domain that mediates contacts with P(II) and with the transcriptional regulator NtcA, to which it binds to increase its activity. Here, we use a combination of in silico, yeast two-hybrid and in vitro approaches to investigate the nitrogen regulation network of Synechococcus WH5701, a marine cyanobacterium with two P(II) (GlnB_A and GlnB_B) and two PipX (PipX_I and PipX_II) proteins. Our results indicate that GlnB_A is functionally equivalent to the canonical P(II) protein from Synechococcus elongatus. GlnB_A interacted with PipX and NAGK proteins and stimulated NAGK activity, counteracting arginine inhibition. GlnB_B had only a slight stimulatory effect on NAGK activity, but its potential to bind effectors and form heterotrimers in Synechococcus WH5701 indicates additional regulatory functions. PipX_II, and less evidently PipX_I, specifically interacted with GlnB_A and NtcA, supporting a role for both Synechococcus WH5701 PipX proteins in partner swapping with GlnB_A and NtcA.

US$19.90

PMID: 21183574

DOI: 10.1099/mic.0.047266-0


Other references

Oppeltz, R.F.; Zhang, Q.; Rani, M.; Sasaki, J.R.; Schwacha, M.G., 2010: Increased expression of cardiac IL-17 after burn. Cardiac dysfunction is a common complication associated with major burns. While recent findings have linked the Th-17 T-cell response to the development of autoimmune myocarditis, the role of IL-17 and the Th-17 T-cell response in the development...

Nwokonko, R.M.; Cai, X.; Loktionova, N.A.; Wang, Y.; Zhou, Y.; Gill, D.L., 2017: The STIM-Orai Pathway: Conformational Coupling Between STIM and Orai in the Activation of Store-Operated Ca 2+ Entry. Store-operated Ca<sup>2+</sup> entry fulfills a crucial role in controlling Ca<sup>2+</sup> signals in almost all cells. The Ca<sup>2+</sup>-sensing stromal interaction molecule (STIM) proteins in the endoplasmi...

Suppiramaniam, V.; Subramaniam, T.; Kanju, P.M.; Kleene, R.; Moss, A.; Premkumar, L.; Dityatev, A., 2001: Single channel recordings from mossy fiber synaptosomes Evidence for the presence of high affinity synaptic AMPA receptors. AMPA receptors are primary mediators of fast excitatory neurotransmission. Previous studies have demonstrated the existence of low and high affinity AMPA receptors and the former being widely present in synaptic plasma membrane. Our preliminary da...

Carey D.E.; Reuben R.; Myers R.M., 1969: Japanese encephalitis studies in vellore south india part 5 experimental infection and transmission. Indian Journal of Medical Research 57(2): 282-289

Shonkoff, J.P., 1990: Point-counterpoint. Pediatrics in Review 11(9): 261-261

Trowbridge, A.H., 1937: Ecological observations on Amphibians and Reptiles collected in Southeastern Oklahoma during the summer of 1934. American Midland Naturalist, 182: 285-303

Hanfman, Dt, 1987: Tilapia culture, 1970-March 1987. Quick bibliography series National Agricultural Library US: 87 (87-45)

Mendenhall, C.E., 1931: Recent Developments In Photoelectricity. Science 73(1883): 107-113

Bauer, K.; Ryberg, T.; Fuis, G.S.; Lueth, S., 2013: Seismic imaging of the Waltham Canyon Fault, California; comparison of ray-theoretical and Fresnel volume prestack depth migration. Near-vertical faults can be imaged using reflected refractions identified in controlled-source seismic data. Often theses phases are observed on a few neighboring shot or receiver gathers, resulting in a low-fold data set. Imaging can be carried o...

Shin, K.T.mita, M.L.nnerdal, B., 2000: Identification of lactoperoxidase in mature human milk. Myeloperoxidase (MPO) derived from milk leukocytes and lactoperoxidase (LPO) secreted from the mammary gland have been identified previously in human colostrum. These peroxidases are known to play host defensive roles through antimicrobial activit...