geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

The phytoalexin camalexin induces fundamental changes in the proteome of Alternaria brassicicola different from those caused by brassinin


, : The phytoalexin camalexin induces fundamental changes in the proteome of Alternaria brassicicola different from those caused by brassinin. Fungal Biology 118(1): 83-93

Camalexin is the major phytoalexin produced by Alternaria thaliana, but is absent in Brassica species that usually produce phytoalexin blends containing brassinin and derivatives. The protein profiles of A. brassicicola treated with camalexin were evaluated using proteomics and metabolic analyses and compared with those treated with brassinin. Conidial germination and mycelial growth of A. brassicicola in liquid media amended with camalexin and brassinin showed that fungal growth was substantially slower in presence of camalexin than brassinin; chemical analyses revealed that A. brassicicola detoxified camalexin at much slower rate than brassinin. Two-dimensional gel electrophoresis (2-DE) followed by tryptic digestion and capillary liquid chromatography-mass spectrometric analyses identified 158 different proteins, of which 45 were up-regulated and 113 were down-regulated relative to controls. Venn diagram analyses of differentially expressed proteins in cultures of A. brassicicola incubated with camalexin and brassinin indicated clear differences in the effect of each phytoalexin, with camalexin causing down-regulation of a larger number of proteins than brassinin. Overall, results of this work suggest that each phytoalexin has several different targets in the cells of A. brassicicola, and that camalexin appears to have greater potential to protect cultivated Brassica species against A. brassicicola than brassinin.

US$19.90

PMID: 24433679

DOI: 10.1016/j.funbio.2013.11.005


Other references

Huet, P-Michel.; Giroux, L.; Laurens, M.; Crenesse, D., 2008: Effect of cold ischemia-warm reperfusion on the cirrhotic rat liver. Cirrhosis is known to induce capillarization of the sinusoidal endothelial cells (SECs) and collagenization of the space of Disse, resulting in a reduced access of plasma and plasma-dissolved substances to hepatocytes due to their limited diffusio...

Grima, R.; Krassas, A.; Bagan, P.; Badia, A.; L.P.mpec Barthes, Fçoise.; Riquet, M., 2010: Treatment of complicated pulmonary aspergillomas with cavernostomy and muscle flap: interest of concomitant limited thoracoplasty. Lung resection for complex aspergilloma (CA) carries high morbidity and mortality and remains controversial in high-risk patients. Cavernostomy followed by muscle-flap plombage has been recommended for patients considered unfit for resection, but...

Schoenweger, G.; Gasser, P.; Hofer, R.; Dalla Via, J., 2004: The population of spawners of grayling Thymallus thymallus L in the Falschauer river South Tyrol, Italy Der Laichbestand der Aesche Thymallus thymallus L in der Falschauer Suedtirol, Italien. A natural population of grayling (Thymallus thymallus) was studied in the Falschauer river (South Tirol, Italy) during its spawning season in spring 2000. Between two samplings on March26 (638fish) and April 8 (245 fish), no significant difference...

Zhu, K.; Brubaker, G.; Smith, J.D., 2007: Large disk intermediate precedes formation of apolipoprotein A-I-dimyristoylphosphatidylcholine small disks. Small approximately 8.5 nm disks formed spontaneously when dimyristoylphosphatidylcholine (DMPC) large unilamellar vesicles (LUVs) were incubated with apolipoprotein A-I (apoA-I) (100:1 molar ratio). However, in a time course study, the transient...

Lombardini, L.; Flore, J.A.; Everard, J.D.; Tattini, M., 1997: Study of growth analysis, water relations, gas exchange, and carbon partitioning in one plum species during water stress and relief. In a greenhouse study in Michigan, USA, in 1995, 1-year-old, potted trees of Prunus insititia (St. Julian; commonly used as a plum rootstock) were subjected to 3 irrigation regimes; 100%, 50% (mild stress, MS) and 25% (severe stress, SS) of field...

Archard, G., A.; Cuthill, I., C.; Partridge, J., C., 2006: Condition-dependent mate choice in the guppy: a role for short-term food restriction?. Condition-dependent mate choice may alter both the strength and direction of sexual selection within species. In this study, the role of short-term food restriction on the mate choice, of female guppies was investigated. Food supply to females was...

Voigt, R.L., 1961: Hybrid forage sorghums in Arizona. Data are given on the performance of several varieties and hybrids. Lindsey 101F gave the highest yield, whereas dry-matter content at cutting and lodging resistance were greatest in the standard variety Regular Hegari.

Jamieson, A.M.; Simic-Glavaski, B.; Tansey, K.; Walton, A.G., 1976: Studies of elastin coacervation by quasielastic light scattering. Faraday Discussions of the Chemical Society: 194-204

Erickson, C.C., 2006: Wolff-Parkinson-White algorithms: useful clinical tool or intellectual curiosity?. Journal of Cardiovascular Electrophysiology 17(7): 717-718

Popov, K.K., 1957: On the distribution of amphibian trematodes in eastern pre-Cucasus and the central zone. Unknown