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The solution structure of the transducin-α-uncoordinated 119 protein complex suggests occlusion of the Gβ₁γ₁-binding sites


, : The solution structure of the transducin-α-uncoordinated 119 protein complex suggests occlusion of the Gβ₁γ₁-binding sites. Febs Journal 282(3): 550-561

Uncoordinated 119 protein (UNC119) is a partner of transducin-α subunit (Gαt ) that is essential for transducin trafficking in rod photoreceptors. The interaction is known to involve binding of the acylated N terminus of Gαt to the hydrophobic pocket of UNC119. To gain insights into the mechanism of transducin trafficking, we isolated a highly pure protein complex between myristoylated chimeric Gαt (Gαt *) and UNC119₅₀₋₂₄₀, and examined the solution structure by small angle X-ray scattering and chemical crosslinking. The solution structure of the Gαt -UNC119₅₀₋₂₄₀ complex was derived with rigid body/ab initio modeling against the small angle X-ray scattering data by use of known atomic structures of Gαt and UNC119, and a distance constraint based on the protein crosslinking with p-phenyldimaleimide. The model of the Gαt -UNC119₅₀₋₂₄₀ complex indicates rotation and bending of the N-terminal α-helix of Gαt from its position in the structure of the heterotrimeric G-protein transducin (Gt ). This allows a considerably more compact complex conformation, which also suggests a novel interface involving the switch II/α3-β5 surface of Gαt . Supporting a novel interface, UNC119 was found to bind full-length Gαt * more strongly than the Gαt N-terminal peptide. Furthermore, UNC119 competed with the effector molecule phosphodiesterase-6 γ-subunit, which is known to bind to the same surface of Gαt . The solution structure of the Gαt -UNC119 complex suggests that the ability of UNC119 to dissociate Gt subunits and release Gαt from the membrane is attributable to disruption and sterical occlusion of the Gβ₁γ₁-binding sites on Gαt .

US$19.90

PMID: 25425538

DOI: 10.1111/febs.13161


Other references

Imajima, M., 1992: Systematics ecology of the Japanese polychaetes - (39). Spionidae - genus Prionospio (Prionospio) (2). Aquabiology (Tokyo): 143: 190-192

Banerjee, B.D.; Zaidi, S.S.; Pasha, S.T.; Rawat, D.S.; Koner, B.C.; Hussain, Q.Z., 1997: Levels of HCH residues in human milk samples from Delhi, India. Bulletin of Environmental Contamination and Toxicology 59(3): 403-406

Busch, M.; Thoma, R.; Schiller, I.; Corboz, L.; Pospischil, A., 2000: Occurrence of chlamydiae in the genital tracts of sows at slaughter and their possible significance for reproductive failure. The aim of this study was to investigate further the role of chlamydiae as pathogens in the genital tracts of sows at slaughter. Genital tracts of 101 randomly selected sows were collected over a period of 11 months in Central and Eastern Switzerl...

Tonnesen H.H., 1989: Studies on curcumin and curcuminoids xiii catalytic effect of curcumin on the peroxidation of linoleic acid by 15 lipoxygenase. International Journal of Pharmaceutics (Kidlington) 50(1): 67-70

Nagy, M., 1992: Comment on the question of medical education. Orvosi Hetilap 133(15): 947-947

Mcgall, G.; Forman, J.E.ic, 2004: Functionalized silicon compounds and methods for their synthesis and use. Provided are functionalized silicon compounds and methods for their synthesis and use. The functionalized silicon compounds include at least one activated silicon group and at least one derivatizable functional group. Exemplary derivatizable funct...

Callahan, J.J., 1948: Interesting notes on bipartite patellae. United States Naval Medical Bulletin 48(2): 229-233

Armsen, T.R.; Joppich, R.; Schubert, G.; Edel, H.H., 1975: Single nephron study of intrarenal urea handling in experimental pyelonephritis. Micropuncture experiments were carried out in 20 rats with bilat. experimental chronic pyelonephritis. Inulin and urea concentrations were estimated in the late proximal, early distal and latte distal nephron segment. Proximal urea reabsorption di...

Fogel, G.R.; Esses, S.I., 2003: Hip spine syndrome: management of coexisting radiculopathy and arthritis of the lower extremity. Significant lumbar spinal stenosis and lower extremity arthritis may coexist in the elderly. This combination of lumbar stenosis with radiculopathy and lower extremity arthritis may lead to diagnostic uncertainty. To describe the findings of hip s...

Khanahmadi, M.; Miraghaee, S.Sh.; Karimi, I., 2013: Evaluation of the Antioxidant and antimicrobial Properties of Dorema aucheri plant. Iranian Red Crescent Medical Journal 14(10): 684-685