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The switch that does not flip: the blue-light receptor YtvA from Bacillus subtilis adopts an elongated dimer conformation independent of the activation state as revealed by a combined AUC and SAXS study

, : The switch that does not flip: the blue-light receptor YtvA from Bacillus subtilis adopts an elongated dimer conformation independent of the activation state as revealed by a combined AUC and SAXS study. Journal of Molecular Biology 403(1): 78-87

Photoreceptors play an important role in plants and bacteria by converting extracellular stimuli into intracellular signals. One distinct class are the blue-light-sensitive phototropins harboring a light-oxygen-voltage (LOV) domain coupled to various effector domains. Photon absorption by the chromophore within the LOV domain results in an activation of the output domain via mechanisms that are hitherto not well understood. The photoreceptor YtvA from Bacillus subtilis is a bacterial analog of phototropins, consists of an LOV and a sulfate transporter/anti-sigma factor antagonist domain, and is involved in the response of the bacterium to environmental stress. We present here analytical ultracentrifugation studies and small-angle X-ray scattering experiments, showing that YtvA is a dimer. On the basis of these results, we present a low-resolution model of the dimer in the dark and the lit state of the protein. In addition, we show that YtvA does not change its oligomerization state or its overall shape upon light activation.


PMID: 20800068

DOI: 10.1016/j.jmb.2010.08.036

Other references

Roudier, A., 1958: Curculionides de l'archipel de Madere. Revue Francaise d'Entomologie, 25: 199-214

Kasson, B.G.; Bai, S.; Liu, J.; Tobin, C.; Kessel, B., 1993: Characterization of a rapid and sensitive enzyme immunoassay (EIA) for progesterone applied to conditioned cell culture media. Progesterone accumulation in conditioned media is a frequently employed endpoint for in vitro cell culture of steroidogenic cells. Although radioimmunoassay (RIA) has been the predominant method for measurement of progesterone, a number of nonradi...

Khan, S.; Khan, M.I.H., 1983: The extent of apical growth of the bast fibres over their mother initials in some trees of Myrtaceae. The mode and extent of apical growth in bast fibers were studied in 5 tree forms of Myrtaceae. Callistemon citrinus, Eucalyptus concinna, E. maculata, E. microtheca and Eugenia jambolana. The majority of fibers grow in length by both ends, while a...

Becker, H.; Chochon, F.; de Toffol, B.; Defebvre, L.; Léger, J.M.; Pierrot-Deseilligny, C.; Sellal, F., 2003: Report of the 55th meeting of the American Academy of Neurology. Honolulu, March 29-April 6, 2003. Revue Neurologique 159(4): 470-486

Macciocchi, S.N.; Reid, D.B.; Barth, J.T., 1993: Disability following head injury. Disability following head injury varies depending on injury mechanism, neuropathology, and other factors, including medical complications. Mild head injury (Glasgow Coma Scale score 13-15) has been shown to have considerable variability in outcome...

Pallarito, K., 1999: Hospitals and hospitality. Meditrust REIT acquires hotel chain for $3 billion. Modern Healthcare 28(2): 50-50

Siu, L.L., 2007: Clinical trials in the elderly--a concept comes of age. New England Journal of Medicine 356(15): 1575-1576

Frolov, M.; Birchler, J., 1998: Mutation in P0, a dual function ribosomal protein. Genetics 150(4): 1487-1495

Robinson, D.L., 1990: Nitrogen utilization efficiency by major forage grasses in Louisiana. Max. forage yields over a 7-year period on Olivier silt loam soil in Louisiana were obtained with 800 lb N/acre in Cynodon dactylon (9.1 tons forage/acre) and Paspalum dilatatum (6.8 tons/acre), with 715 lb N/acre in Pennisetum americanum (5.8 ton...

Moravcik, E., 1978: Important diseases and pests of tulips. Zahradnictvo 3(12): 569-571