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The use of anisotropic cell sheets to control orientation during the self-organization of 3D muscle tissue


, : The use of anisotropic cell sheets to control orientation during the self-organization of 3D muscle tissue. Biomaterials 34(30): 7372-7380

In some parts of native tissues, the orientation of cells and/or extracellular matrixes is well organized. We know that because anisotropy produces important tissue functions, an appropriate anisotropy needs to be designed to biomimetically construct complex tissue. Here, we show the unique features of anisotropic myoblast sheets for organizing the three-dimensional (3D) orientation of myoblasts and myotubes. Utilizing a micropatterned thermoresponsive surface, human skeletal muscle myoblasts were aligned on the surface, and manipulated as a single anisotropic cell sheet by reducing the culture temperature. Consequently, layering of anisotropic myoblast sheets using gelatin gel allowed 3D myotube constructs to be built up with the desired anisotropy. We also discovered a surprising myoblast behavior. An anisotropic cell sheet placed on top of other cell sheets in fabricating thick tissue was able to change the cell orientation in several layered cell sheets underneath. This self-organization is believed to provide the uniqueness required in designing 3D cell orientation architecture for reconstructed muscle tissue.

US$19.90

PMID: 23849343

DOI: 10.1016/j.biomaterials.2013.06.033


Other references

Arnold, Gnl, 1976: Black-tailed godwit in the Bay of Plenty. Notornis(3): 257-258

Abd-Elmoniem, K.Z.; Tomas, M.Santaularia.; Sasano, T.; Soleimanifard, S.; Vonken, E-Jan.P.; Youssef, A.; Agarwal, H.; Dimaano, V.L.; Calkins, H.; Stuber, M.; Prince, J.L.; Abraham, T.P.; Abraham, M.Roselle., 2012: Assessment of distribution and evolution of mechanical dyssynchrony in a porcine model of myocardial infarction by cardiovascular magnetic resonance. We sought to investigate the relationship between infarct and dyssynchrony post- myocardial infarct (MI), in a porcine model. Mechanical dyssynchrony post-MI is associated with left ventricular (LV) remodeling and increased mortality. Cine, gadoli...

Beliakov, R.V., 1963: A Generator For Mechano-Chemical Oscillations On The Basis Of Muscle Ph. Biofizika 8: 741-743

Cleophas, T.J., 1993: Crossover studies: a modified analysis with more power. Clinical Pharmacology and Therapeutics 53(5): 515-520

Stein, B.A.; Adams, J.S.; Master, L.L.; Morse, L.E.; Hammerson, G.A., 2000: A remarkable array Species diversity in the United States. Stein, Bruce A , Kutner, Lynn S , Adams, Jonathan S Precious heritage: The status of biodiversity in the United States 55-92

Nakajima, H.; Murakami, H.; Araki, Y.; Ambo, H.; Okamoto, S.; Handa, M.; Ikeda, Y., 1991: A case of chronic idiopathic thrombocytopenic purpura with a high titre of platelet membrane-associated antiphospholipid antibodies. British Journal of Haematology 78(3): 456-457

Egloff, R.; Fleck, L.; Simmel, G.; Lazarus, M., 2014: Communication of ideas, intersections and consolidation - Fleck, Simmel, and Völkerpsychologie. Ludwik Fleck’s rediscovery, initiated by Thomas Kuhn, was prominently related to its sociological emphasis. But while this emphasis as such resonated with the sociological circles within science studies, Fleck’s actual sociological position wa...

Adler M.; Hinman D.; Hudson C.S., 1992: Role of muscle fasciculations in the generation of myopathies in mammalian skeletal muscle. The myotoxicity of pyridostigmine bromide was investigated on rat diaphragm nerve-muscle preparations in vitro. Within 2 h of exposure to pyridostigmine (2 .mu.M), diaphragm muscles exhibited ultrastructral alterations characterized by swelling of...

Vettore, L.; Concetta-De-Matteis, M.; Falezza, G.C., 1977: Increased potassium permeability induced in vitro by menadione in normal human red cells. Normal red blood cells [human] preincubated for 75 min with 1.15 mM menadione sodium bisulfite lost K and water on subsequent incubation at 37.degree. C for 24 h without menadione. The K loss was increased by addition of Ca and prevented by additi...

Chuang, C.H.; Stengel, M.R.; Hwang, S.W.; Velott, D.; Kjerulff, K.H.; Kraschnewski, J.L., 2015: Behaviours of overweight and obese women during pregnancy who achieve and exceed recommended gestational weight gain. Excessive gestational weight gain (GWG) is associated with increased risk of pregnancy-related complications, postpartum weight retention, and long-term obesity. Little is known about the behavioural habits of pregnant women who achieve and exceed...