geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

The use of ethanol for preserving proteins and glycoproteins of the trabecular meshwork


, : The use of ethanol for preserving proteins and glycoproteins of the trabecular meshwork. Journal of Glaucoma 2(1): 25-29

We evaluated methods for storage of the trabecular meshwork and irisciliary body for protein and glycoprotein analysis. The trabecular meshwork and irisciliary body of rabbit eyes were microdissected and stored in Laemmli sample buffer, Carnoy's fluid (75% ethanol-25% glacial acetic acid), or 100% ethanol at ambient temperature, 4 degrees C, -20 degrees C, or -80 degrees C for 24 h or 30 days. Fresh and stored tissues were processed for one-dimensional polyacrylamide gel electrophoresis (PAGE) and Western blot using Con A lectin. The protein patterns of stored and fresh tissues as determined by silver-stained polyacrylamide gels were similar. However, ethanol-stored tissues revealed other proteins (MW of 15-30 kD and 150 kD), and the staining intensity and band resolution of lower MW (15-40 kD) were enhanced. The glycosylation patterns of stored and fresh tissues as determined by Con A (recognizes certain N-linked glycoproteins containing mannose and glucose) were also similar, but the ethanol-stored tissues stained more intensely, especially the high (>200 kD) and low (<35 kD) MW ranges. These PAGE results indicate that ethanol storage is useful for preserving and resolving the protein/glycoprotein profiles of the trabecular meshwork.

US$29.90

PMID: 19920479


Other references

Kane, H.F.; Kreiselman, J., 1930: The carbon dioxide content of the blood in the newborn. Blood collected from the umbilical cord out of contact with air was analyzed for CO2. The average values in vol. % were 54.6 for asphyxiated infants, 47.3 for gasping infants, and 44.9 for those born crying. Addition of CO2 to O2 as a resuscitatio...

Boukari; Long; Harris, 2000: Polydispersity during the Formation and Growth of the Stöber Silica Particles from Small-Angle X-Ray Scattering Measurements. The early stages of formation of Stöber silica particles have been investigated in situ during the hydrolysis and condensation of tetraethylorthosilicate under base-ammonia conditions in different alcoholic solvents. Time-resolved ultra-small-ang...

Pezzilli, R.; Barassi, A.; Iammarino, M.Teresa.; d'Eril, G.Melzi., 2013: Is troponin T a useful marker of myocardial damage in acute pancreatitis? A prospective time course study. Digestive and Liver Disease 45(4): 347-348

Schuller Levis G.B.; Recce R.; Rudelli R.D.; Sturman J., 1991: Alterations in the immune system in cats fed diets with excess cystine. As part of an ongoing biochemical study in nutrition we examined blood profiles, serum chemistry, lymphocyte transformation and lymphoid pathology in cats fed a diet containing 5% cystine with and without taurine. Automated blood counts of whole b...

Pearl R.M., 1977: Earth's active crust; how the continents fit together. Earth Science 30(2): 83-84

Bielek, E., 1974: Beitrag zur Ontogenese der Blutbildung bei den Teleostiern 1 Blutbildentwicklung bei der Asche Thymallus thymallus L. Zoologische Jb (Anat): 933: 243-258

Richter, P.; Spierto, F.W., 2003: Surveillance of smokeless tobacco nicotine, pH, moisture, and unprotonated nicotine content. Smokeless tobacco is a complex chemical mixture, including not only the components of the tobacco leaf but also chemicals added during the manufacturing process. Smokeless tobacco contains the addictive chemical nicotine and more than 20 cancer-ca...

Monnier, P.; Fontolliet, C.; Ollyo, J.B., 1994: Endoscopic findings of 100 early-stage esophageal cancers. The morphologic analysis of 100 early squamous cell carcinomas of the esophagus has shown that the barely visible or invisible forms (erythroplakias and occult forms) are predominant. This explains the poor yield of upper gastrointestinal (GI) end...

Warnke, K.; Ploetner, J., 2002: Coleoid classification and the systematic position of Spirula. First results of molecular investigations. Berliner Palaeobiologische Abhandlungen, 1: 97-98

Beveridge, R.; Schuster, M.; Waller, E.; Stuart, R.; Abboud, C.; Cruickshank, S.; Menchaca, D.; Macri, M.; Connaghan, D.G., 1997: Randomized, double-blind, placebo-controlled trial of pegylated recombinant human megakaryocyte growth and development factor in breast cancer patients following autologous bone marrow transplantation. Blood 90(10 SUPPL 1 PART 1): 580A, Nov 15