+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Titanium nanoparticle inhalation induces renal fibrosis in mice via an oxidative stress upregulated transforming growth factor-β pathway

, : Titanium nanoparticle inhalation induces renal fibrosis in mice via an oxidative stress upregulated transforming growth factor-β pathway. Chemical Research in Toxicology 28(3): 354-364

Titanium dioxide nanoparticles (Nano-TiO2) are gradually being used extensively in clinical settings, industry, and daily life. Accumulation studies showed that Nano-TiO2 exposure is able to cause injuries in various animal organs, including the lung, liver, spleen, and kidney. However, it remains unclear whether exposure of Nano-TiO2 by inhalation causes renal fibrosis. Here, we investigated the role of reactive oxygen species (ROS)/reactive nitrogen species (RNS) related signaling molecules in chronic renal damage after Nano-TiO2 inhalation in mice. Mice were treated with Nano-TiO2 (0.1, 0.25, and 0.5 mg/week) or microparticle-TiO2 (0.5 mg/week) by nonsurgical intratracheal instillation for 4 weeks. The results showed that Nano-TiO2 inhalation increased renal pathological changes in a dose-dependent manner. No renal pathological changes were observed in microparticle-TiO2-instilled mice. Nano-TiO2 (0.5 mg/week) possessed the ability to precipitate in the kidneys, determined by transmission electron microscopy and increased serum levels of blood urea nitrogen. The expressions of markers of ROS/RNS and renal fibrosis markers, including nitrotyrosine, inducible nitric oxide synthase, hypoxia inducible factor-1α (HIF-1α), heme oxygenase 1, transforming growth factor-β (TGFβ), and collagen I, determined by immunohistochemical staining were increased in the kidneys. Furthermore, Nano-TiO2-induced renal injury could be mitigated by iNOS inhibitor aminoguanidine and ROS scavenger N-acetylcysteine treatment in transcription level. The in vitro experiments showed that Nano-TiO2 significantly and dose-dependently increased the ROS production and the expressions of HIF-1α and TGFβ in human renal proximal tubular cells, which could be reversed by N-acetylcysteine treatment. Taken together, these results suggest Nano-TiO2 inhalation might induce renal fibrosis through a ROS/RNS-related HIF-1α-upregulated TGF-β signaling pathway.


PMID: 25406100

DOI: 10.1021/tx500287f

Other references

Allen, A.D.; Godoy, J.; Fu, N.; Nagy, M.; Spadaro, S.; Tidwell, T.T.; Vukovic, S., 2008: Spiro-aziridine and bislactam formation from bisketene-imine cycloadditions. Journal of the American Chemical Society 130(8): 2386-2387

Langevin, H.M.; Yandow, J.A., 2002: Relationship of acupuncture points and meridians to connective tissue planes. Acupuncture meridians traditionally are believed to constitute channels connecting the surface of the body to internal organs. We hypothesize that the network of acupuncture points and meridians can be viewed as a representation of the network for...

Konigs, A., 1894: Verzeiohniss von Petrefakten des marinen Oberoligocans aus der Umgegend von Crefeld. Verhandlungen des Vereins Rheinland, 12 Halfte): 519-523

Schultheis, J.R.; Wehner, T.C.; Walters,, 1997: Mixtures of cucumber cultigens affect yield in a multiple-harvest system. Seeds of cucumber (Cucumis sativus L.) cultigens were mixed and compared for fruit yield (Mg cntdot ha-1) and crop value ( /ha). Three cultigen pairs (Gy 14A + M 21, Gy 4 + WI 2757, and 'Regal' + 'Carolina') and five component...

Ni, Y.; Nawabi, H.; Liu, X.; Yang, L.; Miyamichi, K.; Tedeschi, A.; Xu, B.; Wall, N.R.; Callaway, E.M.; He, Z., 2014: Characterization of long descending premotor propriospinal neurons in the spinal cord. The motor function of the spinal cord requires the computation of the local neuronal circuits within the same segments as well as the long-range coordination of different spinal levels. Implicated players in this process are the propriospinal neur...

Momma, H.; Sato, R., 1970: The locomotion behaviour of the disc abalone, Haliotis discus hannai Ino, in a tank. Tohoku J. agric. Res.: 21: 20-25

Merrick, G.S.; Wallner, K.E.; Butler, W.M., 2003: Permanent interstitial brachytherapy for the management of carcinoma of the prostate gland. Purpose: We summarize the permanent prostate brachytherapy literature, including biochemical outcomes, quality of life parameters and areas of controversy. Materials and Methods: The permanent prostate brachytherapy literature was reviewed using M...

Papadopoulos I.; Rendig V.V., 1983: Interactive effects of salinity and nitrogen on growth and yield of tomato lycopersicon esculentum cultivar vf 145 plants. Tomato plants were grown with Typic Xerofluvents soil in a greenhouse irrigated with recycled nutrient solutions having increasing levels of N and salinity. Positive response of plants to increasing levels of N was obtained at the lowest initial s...

Andersen O.; Sorensen A.M.; Svehag S E.; Fenouillet E., 1991: Interactions between the mammalian lectin conglutinin and the envelope glycoprotein of hiv 1. Istituto Superiore Di Sanita Vii International Conference on Aids: Science Challenging Aids; Florence, Italy, June 16-21, 464p (Vol 1); 460p (Vol 2) Istituto Superiore Di Sanita: Rome, Italy Paper : 113b

L.T.eut, C.; Rome, Aélique.; Cassagneau, P.; Fernandez, C.; Triglia, J-Michel.; Nicollas, R., 2013: Rhabdomyosarcoma of Stensen's duct in children. Stensen's duct is a very uncommon location for rhabdomyosarcoma. The purpose of this article was to review the clinical history of 2 patients who had rhabdomyosarcoma of Stensen's duct. We reviewed the clinical history, imaging studies,...