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Toll-like receptor 2, 3, 4, 5 ligands and interleukin-4 synergistically induce TARC production in nasal polyp fibroblasts

, : Toll-like receptor 2, 3, 4, 5 ligands and interleukin-4 synergistically induce TARC production in nasal polyp fibroblasts. Auris, Nasus, Larynx 35(4): 515-520

Although type 2 T helper (Th2) cytokines such as IL-4 and IL-5 play a crucial role in the pathogenesis of chronic sinusitis with allergy, the mechanism underlying the predominance of Th2 cytokines has yet to be clarified. Thymus and activation-regulated chemokine (TARC) has been known to facilitate the recruitment of Th2 polarized cells, resulting in high levels of Th2 cytokines in the sinus mucosa as well as nasal polyps. The nasal and sinus cavities are ideal sites for studying the interplay between microbial Toll-like receptor (TLR) ligands and chemokines. We investigated whether nasal polyp fibroblasts produce TARC when stimulated with the breakdown products of microorganisms (TLR ligands) and a Th2 cytokine (IL-4). Fibroblast lines were established from nasal polyp tissues. The expression of TARC mRNA was evaluated by real-time RT-PCR. The amount of TARC in the supernatants was measured by ELISA. Combined stimulation with TLR 2, 3, 4, 5 ligands and IL-4 induced TARC gene expression and protein production in the cultured nasal polyp fibroblasts. This response was time-dependent. These results suggest that nasal polyp fibroblasts contribute to innate immunity and may play an important role in the recruitment of Th2 cells into nasal polyps through the production of TARC.


PMID: 18375080

DOI: 10.1016/j.anl.2008.02.001

Other references

Olgun P.; Gurses C., 1986: Relationships between somatotypes and untrained physical abilities. Day, J A P (Ed ) The 1984 Olympic Scientific Congress Proceedings, Vol 1 Perspectives in Kinanthropometry; Meeting, Eugene, Oreg , Usa, July 19, 1984 Xvi+284p Human Kinetics Publishers, Inc : Champaign, Ill , Usa Illus 115-122

Betz, W.J.; Caldwell, J.H.; Harris, G.L.; Kinnamon, S.C., 1986: A steady electric current at the rat neuromuscular synapse. Progress in Clinical and Biological Research 210: 205-212

Harvey H.A.; Lipton A.; Max D.T.; Pearlman H.G.; Diaz Perches R.; D.L.G.rza J., 1985: Medical castration produced by the gonadotropin releasing hormone analog leuprolide to treat metastatic breast cancer. Leuprolide (Lupron, TAP Pharmaceuticals, North Chicago), a gonadotropin-releasing hormone analog, was administered to 26 premenopausal women with metastatic breast cancer. Of 25 evaluable patients, 11 (44%) had a partial response with a median dur...

Calder, N.A.; Williams, B.A.; Kumar, P.; Hanson, M.A., 1994: Respiratory chemoreflex response of infants to alternate breaths of air and F-I,O2O cntdot 16 at two ages. Journal of Physiology (Cambridge) 476P(0): 80P

Armstrong R.H.; Common T.G.; Smith H.K., 1986: The voluntary intake and in vivo digestibility of herbage harvested from indigenous hill plant communities. Voluntary intake [by cattle and sheep] and in vivo digestibility of herbage harvested from five indigenous communities-Agrostis-Festuca, Nardus and Molinia grasslands and Eriophorum vaginatum- and Trichophorum caespitosum-dominated varieties of bl...

Anonymous, 1888: Remedies for Uterine Hæmorrhage. Indian Medical Gazette 23(1): 17-17

Lenfant, J., 1948: Horticultural education in France and abroad. A broad review of elementary and higher education in horticulture in France, Algeria, Morocco, England, Belgium, Denmark and the Netherlands.

Samir, Ashraf M., 1995: Paleoenvironmental significance of the Upper Cretaceous-Lower Tertiary foraminifera of the North Gunna section, Farafra Oasis, Western Desert, Egypt. The paleoenvironmental conditions of deposition, i.e., paleodepth and paleotemperature, of the Lower Maastrichtian to Lower Eocene sediments of the North Gunna section in the Farafra Oasis (Western Desert, Egypt), are discussed on the basis of som...

van Duyvenvoorde, H.A.; Kempers, M.J.E.; Twickler, T.B.; van Doorn, J.; Gerver, W.J.; Noordam, C.; Losekoot, M.; Karperien, M.; Wit, J.M.; Hermus, A.R.M.M., 2008: Homozygous and heterozygous expression of a novel mutation of the acid-labile subunit. Acid-labile subunit (ALS) deficiency due to homozygous inactivation of the ALS gene (IGFALS) is associated with moderate short stature, and in few cases pubertal delay. The clinical expression of heterozygosity is unknown. To investigate the clini...

Bautista-Hernandez, V.; Iglesias, M.C.; Portela, F., 2012: Use of a donor heart with pre-transplant percutaneous patent foramen ovale closure. Journal of Heart and Lung Transplantation 31(7): 788-789