geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Topographical guidance of 3D tumor cell migration at an interface of collagen densities


, : Topographical guidance of 3D tumor cell migration at an interface of collagen densities. Physical Biology 10(6): 065004-065004

During cancer progression, metastatic cells leave the primary tumor and invade into the fibrous extracellular matrix (ECM) within the surrounding stroma. This ECM network is highly heterogeneous, and interest in understanding how this network can affect cell behavior has increased in the past several decades. However, replicating this heterogeneity has proven challenging. Here, we designed and utilized a method to create a well-defined interface between two distinct regions of high- and low-density collagen gels to mimic the heterogeneities in density found in the tumor stroma. We show that cells will invade preferentially from the high-density side into the low-density side. We also demonstrate that the net cell migration is a function of the density of the collagen in which the cells are embedded, and the difference in density between the two regions has minimal effect on cell net displacement and distance travelled. Our data further indicate that a low-to-high density interface promotes directional migration and induces formation of focal adhesion on the interface surface. Together, the current results demonstrate how ECM heterogeneities, in the form of interfacial boundaries, can affect cell migration.

US$19.90

PMID: 24304905

DOI: 10.1088/1478-3975/10/6/065004


Other references

Yu, G.; Fassler, J.S., 1993: SPT13 (GAL11) of Saccharomyces cerevisiae negatively regulates activity of the MCM1 transcription factor in Ty1 elements. The Ty transposable elements of Saccharomyces cerevisie consist of a single large transcription unit whose expression is controlled by a combination of upstream and downstream regulatory sequences. Errede (B. Errede, Mol. Cell. Biol. 13:57-62, 199...

Adamakopoulos, T.; Giannatos, G.; Hecht Markou, P.; Gaetlich, M.; Ioannidis, J.; Kouroutos, V.; Legakis, A.; Mertzanis, G.; Bousbouras, D., 1992: Threatened species Mammals Group I Insectivora, Lagomorpha, Rodentia, Carnivora, Artiodactyla. Karandinos, M , Paraschi, L The Red Data Book of threatened vertebrates of Greece : 259-302

Romkes, M.; Faletto, M.B.; Blaisdell, J.A.; Raucy, J.L.; Goldstein, J.A., 1991: Cloning and expression of complementary DNAs for multiple members of the human cytochrome P450IIC subfamily. The present study characterizes the profile of cDNAs from the human P450IIC subfamily in a library from one individual, and it describes three new members of this subfamily (IIC17, IIC18, and IIC19) isolated from two human cDNA libraries. cDNA lib...

Aniol, A.; Weznikas, T., 1975: Influence of nitrogen fertilization on the composition of nitrogen fractions in cereal grain. In field trials in 1971, rye, oats, barley and wheat were given 60, 120, 240, 360, 480 or 600 kg N/ha. Data on the amount and composition of N fractions in grain showed that though N content increased with all treatments, the amounts between fract...

Gopinath, B.; Flood, V.M.; Rochtchina, E.; Baur, L.A.; Smith, W.; Mitchell, P., 2012: Influence of high glycemic index and glycemic load diets on blood pressure during adolescence. We aimed to prospectively examine the association between the glycemic index and glycemic load of foods consumed and the dietary intakes of carbohydrates, sugars, fiber, and principal carbohydrate-containing food groups (eg, breads, cereals, and s...

Martinez, O.; Martinez, M.; D.S.usa, J.; Bonardo, P.; Adamson, J.; Reisin, R.; Allegri, R.; Taratutto, A.; Thomson, A.; Fernandez Pardal, M.M., 1997: Creutzfeldt-Jakob disease with unilateral parieto occipital cortical abnormalities on MRI. Journal of the Neurological Sciences 150(SUPPL ): S56-S57

Siripurapu, P.; Kankanamge, D.; Ratnayake, K.; Senarath, K.; Karunarathne, A., 2017: Two independent but synchronized Gβγ subunit-controlled pathways are essential for trailing-edge retraction during macrophage migration. Chemokine-induced directional cell migration is a universal cellular mechanism and plays crucial roles in numerous biological processes, including embryonic development, immune system function, and tissue remodeling and regeneration. During the mi...

Nemtsov, V.; Abol'-, P.; Vinogorov, G., 1979: Modern felling machines. Lesnaia promyshlennost': 0) 28-29

Hegde, R.J.; Sood, P.B., 2003: Dental maturity as an indicator of chronological age: radiographic evaluation of dental age in 6 to 13 years children of Belgaum using Demirjian methods. The study was designed to determine dental age from orthopantomograph using Demirjian method and to investigate applicability of Demirjian method for estimation of chronological age in children of Belgaum. The sample for the study consisted of 197...

Carlsson, A.S., 1997: Radio-opaque agents in bone cement increase resorption. Journal of Bone and Joint Surgery. British Volume 79(6): 1037-1037