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Transfection of exogenous gene: Lac Z into spinal cord of SD rats and its protein expression:an in vivo study

, : Transfection of exogenous gene: Lac Z into spinal cord of SD rats and its protein expression:an in vivo study. Zhongguo Gu Shang 25(1): 47-50

To explore a way of the gene therapy for acute spinal cord injury (ASCI) by vivo transfection of exogenous gene into spinal cord tissue. Twenty-four rats of SD were divided into experiment group and control group (each group had 12 rats). After anaesthesia by abdominal cavity, lamina of thoracic vertebra of all rats were cut-open in prone position. Complex of plasmid and report gene-Lac Z, and plasmid without report gene-Lac Z were respectively injected into cavum subdural of SD rats of experiment group and control group by cation liposome (DOTAP) encapsulation. The rats were killed at the 2nd week after operation, spinal cord tissue of injected segments were detected by reverse transcription-polymerase chain raction (RT-PCR) and immunohistochemistry. In experiment group, positive staining of beta-galactosidase can be clearly observed in neuron and glia cell of rat's spinal cord by immunohistochemistry detection. Lac Z mRNA in same area was also detected by RT-PCR. But, in control group, no above-mentioned positive results were found. Effective transfection of exogenous gene in vivo into spinal cord is a new hot spot for treatment of SCI. Thus certain nerve growth factor imput partly area of spinal cord injury can promote central nerve regrowth and avoid early secondary injury.


PMID: 22489523

Other references

Henderson, Ar, 1984: Long term monitoring of the macrobenthos of the Upper Clyde Estuary. Water science and technology6(5-7): 359-373

Lee, R.T., 2004: Ask the doctor. I've been told that I have an abnormal T wave. Should I be worried about this? How will it affect my heart health?. Harvard Heart Letter 14(6): 8-8

Eisenberg, L., 1971: Professional performance of women physicians. Science 172(3980): 218-219

Cavalcanti,, 2009: Comments on the Family Health Strategy and the Brazilian Unified Health System (SUS), promoted by Sousa and Hamann's text. Ciencia & Saude Coletiva 14 Suppl 1: 1338-40; Discussion 1343-5

Singer, R.; Kristen, K.; Beedgen, B., 1987: The oromandibular limb hypogenesis syndrome. Oroacral syndrome. Deutsche Zeitschrift für Mund-, Kiefer- und Gesichts-Chirurgie 11(3): 149-156

Seifert, R.; King, A.J.o, 1982: Identification of some volatile constituents of Aspergillus clavatus for purpose of differentiating strains of fungi in foods or stored crops. Journal of agricultural and food chemistry 30(4): 786-790

Vahvelainen, M.L.; Oja, S.S., 1972: Kinetics of influx of phenylalanine, tyrosine, tryptophan, histidine and leucine into slices of brain cortex from adult and 7-day-old rats. Brain Research 40(2): 477-488

Bruce, J.; Drysdale, E.M., 1994: Trans-shell transmission. Board, R G [Editor], Fuller, R [Editor] Microbiology of the avian egg 63-91

Fernando, R.S.; Regas, J.; Betz, G., 1988: Ovulation prediction by monitoring salivary electrical resistance with the CUE Fertility Monitor. Obstetrics and Gynecology 72(2): 282-283

Sussman, S.; Garcia, R.; Cruz, T.Boley.; Baezconde-Garbanati, L.; Pentz, M.Ann.; Unger, J.B., 2014: Consumers' perceptions of vape shops in Southern California: an analysis of online Yelp reviews. E-cigarettes are sold at many different types of retail establishments. A new type of shop has emerged, the vape shop, which specializes in sales of varied types of e-cigarettes. Vape shops allow users to sample several types. There are no empiric...