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Transition from enantioselective high performance to ultra-high performance liquid chromatography: A case study of a brush-type chiral stationary phase based on sub-5-micron to sub-2-micron silica particles


, : Transition from enantioselective high performance to ultra-high performance liquid chromatography: A case study of a brush-type chiral stationary phase based on sub-5-micron to sub-2-micron silica particles. Journal of Chromatography. A 1217(7): 990-999

Three brush-type chiral stationary phases (CSPs) differing in the particle size of the starting silica particles have been prepared by covalent grafting of the pi-acidic bis-(3,5-dinitrobenzoyl)-derivative of trans-1,2-diaminocyclohexane (DACH-DNB). Starting silica particles of 4.3, 2.6 and 1.9micron were used to generate the final CSPs using an improved, highly reproducible synthetic methodology, that allowed to assemble and surface-graft the whole chiral selector in only two steps. The different CSPs have been packed in columns of various length and diameters, and fully characterized in terms of flow permeability, kinetic performances and enantioselectivity using a set of test solutes. Very high speed and high resolution applications together with stereodynamic HPLC examples are demonstrated on the columns with reduced particle diameters, on which separations of several enantiomeric pairs are routinely obtained with analysis times in the 15-40s range.

US$19.90

PMID: 19875125

DOI: 10.1016/j.chroma.2009.10.021


Other references

Vancaillie, T.G.; Mitchiner, R.K.; Newton, D.W., 1999: Method of surgical fluid analysis. Official Gazette of the United States Patent & Trademark Office Patents 1226(3), Sep 21

Zawilska, K.; Komarnicki, M.; Mańka, B., 1978: Platelet function in acute myeloid leukemia. II. Aggregation of isolated platelets. In 22 patients with acute myeloid leukaemia (17 cases of myeloblastic leukaemia, 4 cases of myelomonocytic leukaemia and 1 case of undifferentiated-cell leukaemia) platelets were isolated from the plasma by the method of Nicholls and Hampton as mo...

Memish, Z.A.; Ahmed, Q.A.; Arabi, Y.; Shibl, A.M.; Niederman, M.S.; Niederman, M.S.; Garber, G.; Roth, V.R.; Ahmed, Q.A.; Al Knawy, B.; Khoja, T.; Memish, Z.A.; Al-Hosni, M.; Alawaidy, S.; Almoosa, H.; Al Ansari, H.; Abdulatif Shaar, L.; Abdulaziz Abdullah, N.; Alansari, N.; Balkhy, H.H.; Francis, C.; Makki, S.; Al Rabeeah, F.; Al-Zahrani, M.H.; Al Wakeel, M.; Al Hebrawy, A.; Pittet, D.; Memish, Z.A.; Arabi, Y.M.; Shibl, A.; Al Shimemeri, A.; Alghofaili, F.; Al Jeraisy, M.; Al Jahdali, H.; Al Ra, 2008: Rationale for producing evidence-based guidelines for community-acquired pneumonia in the Gulf Corporation Council. World-wide community-acquired pneumonia (CAP) is a common respiratory tract infection and is now a growing public health concern in the GCC region. Practice guidelines are derived statements which lead to informed clinical decision making. Nationa...

Hauck, E.F.; Hoffmann, J.; Schin, M.; Kempski, O., 2003: Effect of Endothelin - A receptor antagonist bsf 208075 on leukocyte - endothelium - interactions and neuronal cell death after transient global cerebal ischemia assessed by intravital videomicroscopy. Endothelin-1 released during reperfusion after stroke may contribute to secondary brain injury. We investigated the cerebral microcirculation after global ischemia in 24 gerbils. Either normal saline (NS) or the new endothelin-A receptor antagonis...

Muschol, N.; Storch, S.; Ballhausen, D.; Beesley, C.; Westermann, J.-Christoph; Gal, A.; Ullrich, K.; Hopwood, J., J.; Winchester, B.; Braulke, T., 2004: Transport, enzymatic activity, and stability of mutant sulfamidise (SGSH) identified in patients with mucpolysaccharidoslis type III A. Mucopolysaccharidosis type IIIA (MPSIIIA) is an autosomal recessive lysosomal storage disease caused by mutations in the N-sulfoglucosamine sulfohydrolase gene (SGSH; encoding sulfamidase, also sulphamidase) leading to the lysosomal accumulation a...

Seaton B.A.; Concha N.O.; Head J.F.; Dedman J.R.; Kaetzel M.A., 1990: Crystallographic studies of rat kidney annexin v. Journal of Cell Biology 111(5 PART 2): 356A

Ruppersberg, J.P.; Burnashev, N.; Guenther, W.; Kuner, T.; Monyer, H.; Sakmann, B.; Seeburg, P.H.; Schoepfer, R., 1993: Mechanism of magnesium block identified by single-channel recording from mutated NMDA-receptor channels. Biophysical Journal 64(2 PART 2): A115

Gaud, J.; Atyeo, WT., 1982: La famille Freyanidae, Dubinin (Sarcoptiformes plumicoles, Freyanoidea) 2. Sous-famille Michaeliinae. Michaeliinae, Gaud and Mouchet 1959, subfamily of Freyanidae, include 3 genera: Michaelia Trouessart 1884, Sulanyssus Dubinin 1953 and MORINYSSUS gen. nov. This genus and its type-species, M. simplex sp. nov., are described from Morus spp.

Gonzálvez, M.; Ruiz Ros, Jé.A.; Pérez-Paredes, Mías.; Lozano, Mía.L.; Giménez, D.M.; Martínez-Corbalán, F.; Carnero, Aés.; Cubero, Tás.; Gómez, A.E.; Vicente, V., 2004: Effect of the early administration of pravastatin on C-reactive protein and interleukin-6 levels in the acute phase of myocardial infarction with ST segment elevation. C-reactive protein (CRP), whose synthesis in the liver is regulated by interleukin 6 (IL-6), is related with the prognosis for ischemic heart disease. The aim of this study was to evaluate the effect of early administration of pravastatin on plasm...

Perbal,B., 2008: Avian Myeloblastosis Virus only one side of the coin. For many years, scientists and suppliers have refered to AMV-RT as the reverse transcriptase produced by the Avian Myeloblastosis Virus. This manuscript briefly reviews the molecular basis for biological dependence of AMV for the envelope and RT p...