geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Transplantation of human mesenchymal stem cells promotes functional improvement and increased expression of neurotrophic factors in a rat focal cerebral ischemia model


, : Transplantation of human mesenchymal stem cells promotes functional improvement and increased expression of neurotrophic factors in a rat focal cerebral ischemia model. Journal of Neuroscience Research 88(5): 1017-1025

Previous studies have suggested that intravenous transplantation of mesenchymal stem cells (MSCs) in rat ischemia models reduces ischemia-induced brain damage. Here, we analyzed the expression of neurotrophic factors in transplanted human MSCs and host brain tissue in rat middle cerebral artery occlusion (MCAO) ischemia model. At 1 day after transient MCAO, 3 x 10(6) immortalized human MSC line (B10) cells or PBS was intravenously transplanted. Behavioral tests, infarction volume, and B10 cell migration were investigated at 1, 3, 7, and 14 days after MCAO. The expression of endogenous (rat origin) and exogenous (human origin) neurotrophic factors and cytokines was evaluated by quantitative real-time RT-PCR and Western blot analysis. Compared with PBS controls, rats receiving MSC transplantation showed improved functional recovery and reduced brain infarction volume at 7 and 14 days after MCAO. In MSC-transplanted brain, among many neurotrophic factors, only human insulin-like growth factor 1 (IGF-1) was detected in the core and ischemic border zone at 3 days after MCAO, whereas host cells expressed markedly higher neurotrophic factors (rat origin) than control rats, especially vascular endothelial growth factor (VEGF) at 3 days and epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) at 7 days after MCAO. Intravenously transplanted human MSCs induced functional improvement, reduced infarct volume, and neuroprotection in ischemic rats, possibly by providing IGF-1 and inducing VEGF, EGF, and bFGF neurotrophic factors in host brain.

US$19.90

PMID: 19885863

DOI: 10.1002/jnr.22279


Other references

Ritsner M.S., 1987: Clinical and genetic aspect of the relationship between schizoid personality abnormalities and schizophrenia review of the literature. Zhurnal Nevropatologii i Psikhiatrii Imeni S S Korsakova 87(5): 777-784

McClelland, D.L., 1994: Therapeutic oxygen carriers--state of the art. Vox Sanguinis 67 Suppl 3: 73-75

Erdine, S.; Yucel, A., 1994: Long-term results of intrathecal morphine in 65 patients. Between 1990 and 1992 intrathecal drug delivery systems were used in 65 cancer patients. The long-term results of intrathecal morphine have been evaluated. The mean duration of drug delivery by these systems was 4.7 +- 3.4 months (1-15 months). Th...

Lee Y.J.; Dewey W.C., 1988: Thermotolerance induced by heat sodium arsenite or puromycin its inhibition and differences between 43c and 45c. When CHO cells were treated either for 10 min at 45-45.5.degree. C or for 1 hr with 100 .mu.M sodium arsenite (ARS) or for 2 hr with 20 .mu.g/ml puromycin (PUR-20), they became thermotolerant to a heat treatment at 45-45.5.degree. C administered 4...

Kuoksa T.; Hohtola A., 1991: Freeze preservation of buds from scots pine trees. A procedure has been developed for freeze-preservation of buds of the Scots pine (Pinus sylvestris L.). Instead of liquid nitrogen, cold storage in -80.degree. C was used. The partly dormant material used in the experiments was obtained directly f...

Ruiz-Ruiz, F.J.; Saenz-Abad, D.; Hualde-Enguita, A.M.; Morales-Rull, J.L., 2005: Positron emission tomography: useful in detecting metastatic cancer of unknown primary site. Metastatic cancer of unknown primary site represents approximately three percent of all new cancer diagnoses. Expensive and invasive diagnostic procedures are often performed although the primary tumour is detected in less than 25 percent of cases...

Goel, M.M.; Agrawal, S.P.; Srivastava, A.N., 2003: Salivary duct carcinoma of the larynx: report of a rare case. Salivary duct carcinomas are primarily high-grade, aggressive malignancies that affect men in the fifth and sixth decades of life. These tumors are usually found in the major salivary glands; rarely do they originate in the minor salivary glands....

Anonymous, 1995: Purchasers uncertain of practice nurses' role. NlmCategory="UNASSIGNED">Practice nurses see their wide range of work as a strength, while health authorities and trusts can view it as a problem, the Scientific Basis of Health Services conference heard.

Aizawa, H.; Nobe, K.; Tsukamoto, M.; Ishizaka, R.; Mizobuchi, R.; Momose, K.; Paul, R.J., 2003: Rho kinase mediates serum-induced contraction in fibroblast fibers. Journal of Pharmacological Sciences 91(Supplement I): 80P

Nasu, W.; Kobayashi, S.; Kashiwa, K.; Honda, T., 2008: Secondary craniofacial reconstruction of huge frontoethmoidal encephalomeningocele after primary neurosurgical repair. Frontoethmoidal encephalomeningocele is a congenital herniation of intracranial contents, including meninges, brain and part of the ventricle, through a bony defect in the skull at the junction of the frontal and ethmoid bones. Management involves...