geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Using of metal-affinity chromatography for identification of alkylated rat globin


, : Using of metal-affinity chromatography for identification of alkylated rat globin. Bioorganicheskaia Khimiia 38(1): 52-57

Rat globin peptides alkylated by sulfur mustard on amino-acid residues C-126, C-93 and E-27 with MH+ 1444.62 Da, 1561.66 Da, 1676.78 Da, respectively, were concentrated using metal-affinity chromatography on Cu2+. The peptides were received by trypic digestion after in vitro incubation of rat globin with 60 microM HD. Aklylated peptide with MH+ 1444.62 Da is the most sensitive biomarker, which can be concentrated from globin trypic digest, incubated with 3 microM sulfur mustard.

US$29.90

PMID: 22792705


Other references

Vera-Ramirez, L.; Sanchez-Rovira, P.; Ramirez-Tortosa, M.Carmen.; Ramirez-Tortosa, C.L.; Granados-Principal, S.; Lorente, J.A.; Quiles, J.L., 2012: Free radicals in breast carcinogenesis, breast cancer progression and cancer stem cells. Biological bases to develop oxidative-based therapies. Oxidative stress leads to lipid, carbohydrate, protein and DNA damage in biological systems and affects cell structure and function. Breast cancer cells are subjected to a high level of oxidative stress, both intracellular and extracellular. To su...

Zeiner, A.R.; Schell, A.M., 1971: Individual differences in orienting, conditionability, and skin resistance responsivity. Psychophysiology 8(5): 612-622

Cremerius, J.; Abraham, K.; Freud, S., 1997: Karl Abraham, Freud's scapegoat and, guide to understanding the truth. The history of the relationship between Freud and Abraham is characterized by the presence of third parties. There was always another, more favored one of Freud's disciples between Freud and Abraham. The first of these, from 1907 to 1912, was...

Stace C.A.; Cotton R., 1974: Hybrids between festuca rubra and vulpia membranacea. Watsonia 10(2): 119-138

Orpin, A.; Walsh, J.P.; Corbett, R.; Ogston, A.; Hale, R.; Kiker, J.; Moriarty, J.; Harris, C., 2011: Floods, ocean storms and stratigraphic variability over a year-long experiment on the muddy and energetic Poverty Bay shelf. Geoscience Society of New Zealand Miscellaneous Publication 130: 81

Ko, Y.S.; Kim, J.J.; Han, I.J., 1970: Composition of Korean human and cows' milk. I. Amino acid composition. Regarding free amino acid content, in cows' milk there were higher levels of arginine (7x), aspartic and glutamic acids (-2x), proline (2x) and glycine (3x); in human milk, higher levels of free histidine (15x), threonine, alanine, cystine (3...

Ohashi, H.; Tanaka, K.; Nemoto, T., 1999: Embryological studies in Euchresta japonica (Leguminosae). Embryological features in Euchresta japonica, a species of the monotypic tribe Euchresteae, are described for the first time. Features in microsporogenesis are as follows: the anthers are tetrasporangiate; anther wall development corresponds to th...

Hocking, M.D.; Rayner, P.W.; Nattrass, M., 1987: Residual insulin secretion in adolescent diabetics after remission. Twenty four hour blood glucose profiles were compared in two groups of insulin dependent adolescent diabetic patients who were beyond their initial partial remission phase. In the group with persistent endogenous insulin secretion, blood glucose p...

Clunie, G.J., 1972: Autotransplantation of the kidney. British Journal of Urology 44(6): 723-724

Jacob, J.; Grimmer, G., 1970: Die Burzeldrusenlipide des Rebhuhns (Perdix perdix). Z. Naturf.: 25B 689-692