+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Utility of amniotic cells plus collagen I complex for fetal membrane healing

, : Utility of amniotic cells plus collagen I complex for fetal membrane healing. Zhonghua Yi Xue Za Zhi 90(27): 1933-1935

To study the feasibility of using amniotic cells plus collagen I complex for fetal membrane healing. Human amnion mesenchymal cells were embedded in three-dimensional (3D) collagen I and epithelial cells placed on the top. The amniotic cells plus collagen I complex was created to mimic the architecture of native amnion. As judged by DAPI staining, the number of amniotic cells cultured in 3D collagen I was counted after incubation for 2 and 8 days. The tensile strength of collagen I with a varying number of amniotic mesenchymal cells was measured after a 15-day incubation. At Days 2 and 8, the mean number of mesenchymal cells was (121 ± 5)/cm(2) and (124 ± 4)/cm(2) (P > 0.05). The size of collagen I matrix was reduced to 45%, 15%, 14% at Days 5, 10, 15 respectively. Mesenchymal cell was capable of remodeling collagen I (P < 0.01) and it could not be inhibited by GM6001 and/or aprotinin. The tensile strength of amniotic cells plus collagen I scaffold complex was strengthened with an increasing number of amnion mesenchymal cells (P < 0.01). The amniotic cells plus collagen I complex may be useful for healing preterm premature rupture of the membrane (PPROM).


PMID: 20979916

Other references

Manwaring, W.H., 1934: Linguistic Pitfalls in Clinical Logic. California and Western Medicine 40(3): 197-198

Schouteden, H., 1934: Un genre africain nouveau de Gomphine. KARSCHIOGOMPHUS (p.225), type K. ghesquierei* (p.226), Belgian Congo.

Perez, V.; Corpa, J.M.; Garcia Marin, J.F.; Aduriz, J.J.; Jensen, H.E., 1999: Generalized aspergillosis in dairy sheep. Two cases of systemic aspergillosis are described in dairy sheep from a flock in which fungal mastitis appeared subsequent to the antibiotic treatment of animals before parturition [location and date not stated]. Lesions characterized by necrosis...

Pelcman, B.; Sanin, A.; Nilsson, P.; Schaal, W.; Olofsson, K.; Krog-Jensen, C.; Forsell, P.; Hallberg, A.; Larhed, M.; Boesen, T.; Kromann, H.; Claesson, H-Erik., 2016: N-Substituted pyrazole-3-carboxamides as inhibitors of human 15-lipoxygenase. High-throughput screening was used to find selective inhibitors of human 15-lipoxygenase-1 (15-LOX-1). One hit, a 1-benzoyl substituted pyrazole-3-carboxanilide (1a), was used as a starting point in a program to develop potent and selective 15-LOX...

Schaff, Z.; Lapis, K., 1975: Electron microscopic study of cytoplasmic crystals in liver damages caused by alcoholism. Liver biopsy of 50 patients with liver disease of alcoholic etiology was examined electronmicroscopically. In every stage of the disease presence of intramitochondrial paracrystalloids was revealed. Hyalin of alcoholic origin of fibrillar structur...

Carter, M.C.; Ruiz-Esteves, K.N.; Workman, L.; Lieberman, P.; Platts-Mills, T.A.E.; Metcalfe, D.D., 2017: Identification of alpha-gal sensitivity in patients with a diagnosis of idiopathic anaphylaxis. IgE antibodies (Ab) specific to galactose-α-1,3-galactose (alpha-gal) are responsible for a delayed form of anaphylaxis that occurs 3 to 6 hours after red meat ingestion. In a unique prospective study of seventy participants referred with a diagn...

Menage, H.D.P.; Norris, P.G.; Hawk, J.L.M.; Greaves, M.W., 1993: The efficacy of psoralen photochemotherapy in the treatment of aquagenic pruritus. Psoralen photochemotherapy (PUVA) was effective in the treatment of five patients with aquagenic pruritus, associated in one with polycythaemia rubra vera and in another with the myelodysplastic syndrome. Relapse occurred within 2-24 weeks when tr...

Papi, G.; Corrado, S.; Carani, C.; Asa, S.L., 2005: Metastasis of a caecal neuroendocrine carcinoma to the thyroid gland. Journal of Clinical Pathology 58(12): 1342-1343

MacIsaac, A.I.; McDonald, I.G.; Kirsner, R.L.; Graham, S.A.; Tanzer, D., 1992: Left ventricular energy in mitral regurgitation: a preliminary report. Energy exchange based on Newtonian principles is the most appropriate way to express the function of any pump--including the heart. Using information obtained at cardiac catheterisation, we have measured the total work energy (ET) of the left vent...

Wennmalm, A.; Hedqvist, P., 1970: Prostaglandin E1 as inhibitor of the sympathetic neuroffector system in the rabbit heart. Life Sciences. Pt. 1: Physiology and Pharmacology 9(16): 931-937