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Vacuolar-Iron-Transporter1-Like proteins mediate iron homeostasis in Arabidopsis


, : Vacuolar-Iron-Transporter1-Like proteins mediate iron homeostasis in Arabidopsis. Plos One 9(10): E110468-E110468

Iron deficiency is a nutritional problem in plants and reduces crop productivity, quality and yield. With the goal of improving the iron (Fe) storage properties of plants, we have investigated the function of three Arabidopsis proteins with homology to Vacuolar Iron Transporter1 (AtVIT1). Heterologous expression of Vacuolar Iron Transporter-Like1 (AtVTL1; At1g21140), AtVTL2 (At1g76800) or AtVTL5 (At3g25190) in the yeast vacuolar Fe transport mutant, Δccc1, restored growth in the presence of 4 mM Fe. Isolated vacuoles from yeast expressing either of the VTL genes in the Δccc1 background had a three- to four-fold increase in Fe concentration compared to vacuoles isolated from the untransformed mutant. Transiently expressed GFP-tagged AtVTL1 was localized exclusively and AtVTL2 was localized primarily to the vacuolar membrane of onion epidermis cells. Seedling root growth of the Arabidopsis nramp3/nramp4 and vit1-1 mutants was decreased compared to the wild type when seedlings were grown under Fe deficiency. When expressed under the 35S promoter in the nramp3/nramp4 or vit1-1 backgrounds, AtVTL1, AtVTL2 or AtVTL5 restored root growth in both mutants. The seed Fe concentration in the nramp3/nramp4 mutant overexpressing AtVTL1, AtVTL2 or AtVTL5 was between 50 and 60% higher than in non-transformed double mutants or wild-type plants. We conclude that the VTL proteins catalyze Fe transport into vacuoles and thus contribute to the regulation of Fe homeostasis in planta.

US$19.90

PMID: 25360591

DOI: 10.1371/journal.pone.0110468


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Chegou, N.N.; Hoek, K.G.P.; Kriel, M.; Warren, R.M.; Victor, T.C.; Walzl, G., 2011: Tuberculosis assays: past, present and future. Recent developments in the field of TB diagnostics, including the introduction of the Xpert MTB/RIF assay in field testing, raise the hope for faster and more accurate identification of active TB patients. However, there are still many issues that...

Hay Mark, 1997: The Western Union Mine, Cerbat Mountains, Mohave County, Arizona. The Mineralogical Record 28(3): 167-174

Rogers G.; Kinny P.D.; Strachan R.A.; Friend C.R.L.; Paterson B.A., 2001: U-Pb geochronology of the Fort Augustus granite gneiss; constraints on the timing of Neoproterozoic and Palaeozoic tectonothermal events in the NW Highlands of Scotland. The West Highland granite gneiss suite in Inverness-shire, Scotland, represents a series of S-type, anatectic granites formed by partial melting of host Neoproterozoic metasediments of the Moine Supergroup. U-Pb (SHRIMP) dating of zircons from a m...

Tanasiichuk, V.N., 1963: Biology of Parochthiphila Czerny. The author presents a description of a new sp., Parochthiphila phragmitis and a redescription of Parochthiphila coronata, P. phragmitis larvae are found in the leaf sheath of reeds where they destroy the scale Chaetococcus phragitis. P. coronata l...

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Michael, M., 1957: The patient with fever of unknown origin. Gp 16(4): 74-82

Rennard, S.I.; Locantore, N.; Delafont, B.; Tal-Singer, R.; Silverman, E.K.; Vestbo, Jørgen.; Miller, B.E.; Bakke, P.; Celli, Bé.; Calverley, P.M.A.; Coxson, H.; Crim, C.; Edwards, L.D.; Lomas, D.A.; MacNee, W.; Wouters, E.F.M.; Yates, J.C.; Coca, I.; Agustí, A.; Ivanov, Y.; Kostov, K.; Bourbeau, J.; Fitzgerald, M.; Hernández, P.; Killian, K.; Levy, R.; Maltais, F.; O'Donnell, D.; Krepelka, J.; Vestbo, J.; Wouters, E.; Quinn, D.; Bakke, P.; Kosnik, M.; Agusti, A.; Feschenko, Y.; Gavrisy, 2015: Identification of five chronic obstructive pulmonary disease subgroups with different prognoses in the ECLIPSE cohort using cluster analysis. Chronic obstructive pulmonary disease (COPD) is a heterogeneous disease that likely includes clinically relevant subgroups. To identify subgroups of COPD in ECLIPSE (Evaluation of COPD Longitudinally to Identify Predictive Surrogate Endpoints) sub...

Gumm, I.D.; Taylor, W.P.; Roach, C.J.; Alexander, F.C.; Greiner, E.C.; Gibbs, E.P., 1984: Serological survey of ruminants in some Caribbean and South American countries for type-specific antibody to bluetongue and epizootic haemorrhagic disease viruses. The microneutralization test using the international serotypes 1 to 22 of bluetongue virus detected antibodies of several types in cattle, sheep and goats in Jamaica, Antigua, Barbados, Grenada, Trinidad and Tobago, Guyana and Surinam. In 1981-82...

Crowell John Chambers, 1948: Geology of Hungry Valley area, south of Gorman (California). Bulletin of the American Association of Petroleum Geologists 32(12): 2319

Reiss, M.; Haurowitz, F., 1929: Chemistry of the sexual hormone of the anterior hypophysis lobe. This hormone (extracted from beef hypophysis) is compared with the substance of like biological activity derived from the urine of pregnancy (prepared according to the method of Biedl). Both in adsorption tests and in deportment toward proteoses t...