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Value of microbial gene 16SrRNA in the identification of antenatal infection

, : Value of microbial gene 16SrRNA in the identification of antenatal infection. Zhongguo Dang Dai Er Ke Za Zhi 12(9): 726-729

To study the relationship between microbial gene 16SrRNA and intrauterine infection. Thirty cases of single preterm birth were enrolled, including 16 cases due to premature rupture of membranes (PROM) (rupture time>18 hrs), 6 cases due to spontaneous preterm birth and 8 cases due to iatrogenic preterm birth. Ten cases of single term birth were used as the control group. Fetal membrane and placenta samples were obtained. Amniotic fluid, blood from cord or newborn babies as well as gastric fluid and tracheal secretions from infants with mechanical ventilation were also obtained. The histological features of placenta and fetal membranes were observed. Polymerase chain reaction (PCR) was used to detect the presence of microbial 16SrRNA and ureaplasma urealyticum (UU) in placenta, fetal membranes and other samples. Twenty-one (70%) cases were diagnosed as chorioamnionitis, characterized by neutrophil infiltration in fetal membrane and placenta tissues, especially in fetal membranes. Chorioamnionitis was most frequent in babies whose gestational age less than 32 weeks or birth weight lower than 1 500 g. Positive 16SrRNA gene was found in 12 cases, and positive UU gene in 10 cases in the preterm birth group. Neither 16SrRNA nor UU gene was detected in the control group. The PROM preterm babies developed more frequent infection than the babies premature born due to other causes, but there were no statistically significant differences in the incidence of infection. Chorioamnionitis may be the major cause of PROM and premature birth. The detection of microbial genes is valuable in identification of intrauterine infection.


PMID: 20849724

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Sotiropoulos, G.C.; Fouzas, I.; Paul, A.; Nadalin, S.; Molmenti, E.P.; Valentin-Gamazo, C.; Radtke, A.; Kühl, H.; Malagó, M.; Broelsch, C.E., 2007: Inferior vena cava thrombosis after right hepatectomy for live donor liver transplantation: a major donor complication and a satisfactory treatment modality. American Journal of Transplantation 7(12): 2836-2837

Baren, R.E.; Parrish, M.E.; Shafer, K.H.; Harward, C.N.; Shi, Q.; Nelson, D.D.; McManus, J.Barry.; Zahniser, M.S., 2004: Quad quantum cascade laser spectrometer with dual gas cells for the simultaneous analysis of mainstream and sidestream cigarette smoke. A compact, fast response, infrared spectrometer using four pulsed quantum cascade (QC) lasers has been applied to the analysis of gases in mainstream (MS) and sidestream (SS) cigarette smoke. QC lasers have many advantages over the traditional lea...

Pregazzi, R.; Baraggino, E.; Mazzatenta, E.; Strolego, F., 1984: Changes in the fetal heart rate during the expulsion stage. Significance of terminal bradycardia. Minerva Ginecologica 36(1-2): 7-10

Najeeb A.H.llak, M.; McCurdy, M.; Zouain, N.; Hayes, J., 2009: The Level of Europium-154 Contaminating Samarium-153-EDTMP Activates the Radiation Alarm System at the US Homeland Security Checkpoints. (153)Sm-EDTMP is a radiopharmaceutical composed of EDTMP (ethylenediamine-tetramethylenephosphonate) and Samarium-153 [1]. (153)Sm-EDTMP has an affinity for skeletal tissue and concentrates in areas with increased bone turnover; thus, it is succes...

Benson, A.A.; Stein, J.L.; Nonomura, A.M., 1993: Methanol effect on leaf photorespiration. FASEB Journal 7(7): A1110

Alihonou, E.; Goufodji, S.; Capo-chichi, V., 1996: Morbidity and mortality related to induced abortions (a study conducted in hospitals of Cotonou, Benin in 1993). This study assesses socio-economic burdens of patients bearing complications arising from induced abortions in the hospitals of Cotonou, Benin, and outlying maternities. The 1-year prospective study took place in three centers. Patients included i...

Roy, P.; Valeri, A.; Danziger, R.S., 1996: Dialysate magnesium concentration is linked to intradialytic hypotension. Circulation 94(8 SUPPL ): I95

Dominiak, P.; Mathieu, B., 2015: Serromyia diabolica, a new biting midge species from Lebanon (Diptera: Ceratopogonidae). A new darkly-pigmented species of Serromyia, closely related to S. subinermis Kieffer, is described from both sexes based on morphological characters and COI gene sequence analyses.

Ditmar G.V.; Maksimovskiy V.A.; Sindeyev A.S., 1979: Principles of the homogenous distribution of alkali, uranium and thorium in volcanic zones of the Soviet Far East. Trudy - Vsesoyuznyy Ordena Lenina Nauchno-Issledovatel'skiy Geologicheskiy Institut im AP

Khoroshaeva, R.A.; Sharapov, N.U., 2001: Comparative estimation of the myocardium functional reserves in patients with combined atherosclerotic affection of arteries. Klinichna Khirurhiia: 58-58