geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Variable DNA methylation patterns associated with progression of disease in hepatocellular carcinomas


, : Variable DNA methylation patterns associated with progression of disease in hepatocellular carcinomas. Carcinogenesis 29(10): 1901-1910

Hepatocellular carcinoma (HCC) most commonly arises from chronic inflammation due to viral infection, as a result of genetic and epigenetic abnormalities. A global picture of epigenetic changes in HCC is lacking. We used methylated CpG island amplification microarrays (MCAMs) to study 6458 CpG islands in HCC and adjacent preneoplastic tissues [chronic hepatitis (CH) or liver cirrhosis (LC)] in comparison with normal liver tissues where neither viral infection nor hepatitis has existed. MCAM identified 719 (11%) prominent genes of hypermethylation in HCCs. HCCs arising from LC had significantly more methylation than those arising from CH (1249 genes or 19% versus 444 genes or 7%, P < 0.05). There were four patterns of aberrant methylation: Type I (4%, e.g. matrix metalloproteinase 14) shows a substantially high methylation level in adjacent tissue and does not increase further in cancer. Type II (55%, e.g. RASSF1A) shows progressively increasing methylation from adjacent tissue to HCC. Type III (4%, e.g. GNA14) shows decreased methylation in adjacent tissue but either similar or increased methylation in HCC. Type IV (37%, e.g. CDKN2A) shows low levels of methylation in normal tissue and adjacent tissue but high levels in HCC. These DNA methylation changes were confirmed by quantitative pyrosequencing methylation analysis in representative 24 genes and were analyzed for correlation with clinicopathological parameters in 38 patients. Intriguingly, methylation in the Type IV genes is characteristic of moderately/poorly differentiated cancer. Our global epigenome analysis reveals distinct patterns of methylation that are probably to represent different pathophysiologic processes in HCCs.

US$19.90

PMID: 18632756

DOI: 10.1093/carcin/bgn170


Other references

Scheer, D.; Benighaus, C.; Benighaus, L.; Renn, O.; Gold, S.; Röder, B.; Böl, G-Fleur., 2015: The distinction between risk and hazard: understanding and use in stakeholder communication. A major issue in all risk communication efforts is the distinction between the terms "risk" and "hazard." The potential to harm a target such as human health or the environment is normally defined as a hazard, whereas risk also...

Rodriguez; Gonzalez, J.M., 1942: Psicologia del en-fermo leproso. The author summarizes the various types of psycho-pathological symptoms exhibited by the victims of leprosy that must be recognized and treated in addition to the physical symptoms.

Lassiter J.W., 1969: Manganese 55 absorption balance and liver levels in the rat as affected by dietary calcium and phosphorus. Bulletin of the Georgia Academy of Science 27(2): 75-76

Wang, Yuguang; Chackalamannil, Samuel; Stamford, Andrew; Boyle, Craig D.; Hu, Zhiyong; Lankin, Claire; Clader, John; Xu, Ruo; Asberom, Theodros; Pissarnitski, Dmitri; Greenlee, William, 2003: Discovery of Sch 444877, a potent, selective and orally active cyclic guanine PDE5 inhibitor. Abstracts of Papers American Chemical Society 226(1-2): MEDI 367

García-Sánchez, Jé.Elías.; García-Sánchez, E.; Martín-Del-Rey, Ángel.; García-Merino, E., 2013: Anaerobic bacteria 150 years after their discovery by Pasteur. In 2011 we celebrated the 150th anniversary of the discovery of anaerobic bacteria by Louis Pasteur. The interest of the biomedical community on such bacteria is still maintained, and is particularly focused on Clostridium difficile. In the past f...

Senior, R.J.; Milford, G.N.; Janousek, J.; Dunlop, A.E.; Wagner, K.; Bachor, H-A.; Ralph, T.C.; Huntington, E.H.; Harb, C.C., 2007: Observation of a comb of optical squeezing over many gigahertz of bandwidth. We experimentally demonstrate the generation of optical squeezing at multiple longitudinal modes and transverse Hermite-Gauss modes of an optical parametric amplifier. We present measurements of approximately 3 dB squeezing at baseband, 1.7 GHz, 3...

Thomas, A.; James, B.Rose.; Landsberg, D., 2009: Colonic necrosis due to oral kayexalate in a critically-ill patient. Kayexalate (sodium polystyrene sulfonate in sorbitol) is commonly used for treatment of hyperkalemia. We describe the case of a critically ill patient who developed colonic necrosis after oral administration of kayexalate.

Herold, I.; Szabo, P., 1993: Feeding weaned calves with bitter lupin made alkaloid free by washing with warm acid. Feeding experiments were performed with 60 weaned Simmental x Holstein calves to determine the feeding value and acceptability of bitter lupin grains after washing with warm, diluted phosphoric acid for 24-26 h, or preferably for 48 h. 15% of the...

Singh, S.; Reddicliffe, N.; Parker, D.A., 2008: Patient preparation for intravenous urography: are we practising evidence-based medicine?. To identify the current practice of patient preparation prior to intravenous urography (IVU) in England and Wales. Seventy-two hospitals were contacted to request details regarding the duration of fluid restriction, adherence to a low-residue diet...

Ulbrich, A.G.; Florido, M.P.C.; Nudelman, V.; Reis, E.S.; Baracho, G.V.; Isaac, L., 2001: Hereditary human complement C3 deficiency owing to reduced levels of C3 mRNA. An 8-year-old son (L.A.S.) of consanguineous parents, presented recurrent bacterial infections, vasculitis and extremely low levels of serum C3 (0.15 microg/ml). The classical and alternative pathway haemolytic activities and the generation of ops...