+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Variation in the Subcellular Localization and Protein Folding Activity among Arabidopsis thaliana Homologs of Protein Disulfide Isomerase

, : Variation in the Subcellular Localization and Protein Folding Activity among Arabidopsis thaliana Homologs of Protein Disulfide Isomerase. Biomolecules 3(4): 848-869

Protein disulfide isomerases (PDIs) catalyze the formation, breakage, and rearrangement of disulfide bonds to properly fold nascent polypeptides within the endoplasmic reticulum (ER). Classical animal and yeast PDIs possess two catalytic thioredoxin-like domains (a, a') and two non-catalytic domains (b, b'), in the order a-b-b'-a'. The model plant, Arabidopsis thaliana, encodes 12 PDI-like proteins, six of which possess the classical PDI domain arrangement (AtPDI1 through AtPDI6). Three additional AtPDIs (AtPDI9, AtPDI10, AtPDI11) possess two thioredoxin domains, but without intervening b-b' domains. C-terminal green fluorescent protein (GFP) fusions to each of the nine dual-thioredoxin PDI homologs localized predominantly to the ER lumen when transiently expressed in protoplasts. Additionally, expression of AtPDI9:GFP-KDEL and AtPDI10: GFP-KDDL was associated with the formation of ER bodies. AtPDI9, AtPDI10, and AtPDI11 mediated the oxidative folding of alkaline phosphatase when heterologously expressed in the Escherichia coli protein folding mutant, dsbA-. However, only three classical AtPDIs (AtPDI2, AtPDI5, AtPDI6) functionally complemented dsbA-. Interestingly, chemical inducers of the ER unfolded protein response were previously shown to upregulate most of the AtPDIs that complemented dsbA-. The results indicate that Arabidopsis PDIs differ in their localization and protein folding activities to fulfill distinct molecular functions in the ER.


PMID: 24970193

DOI: 10.3390/biom3040848

Other references

Safár, J.; Bartos, J.; Janda, J.; Bellec, A.; Kubaláková, M.; Valárik, M.; Pateyron, Séphanie.; Weiserová, J.; Tusková, R.; Cíhalíková, J.; Vrána, J.; Simková, H.; Faivre-Rampant, P.; Sourdille, P.; Caboche, M.; Bernard, M.; Dolezel, J.; Chalhoub, B., 2004: Dissecting large and complex genomes: flow sorting and BAC cloning of individual chromosomes from bread wheat. The analysis of the complex genome of common wheat (Triticum aestivum, 2n = 6x = 42, genome formula AABBDD) is hampered by its large size ( approximately 17 000 Mbp) and allohexaploid nature. In order to simplify its analysis, we developed a gener...

Powell, H.R.F.; Birman, C.S., 2016: Large vestibular aqueduct syndrome: Impedance changes over time with different cochlear implant electrode arrays. The aim of this study was to assess if large vestibular aqueduct syndrome (LVAS), with the increase in perilymphatic pressure, affects impedance changes over time with different types of Cochlear(®) implant electrode arrays Contour, Straight, and...

Pelleg, A.; Geva, B.; Bersovzky, A.; Dabush, S.; Messer, G., 1983: Ultrastructural localization of glucose-6-phosphatase in renal glomerulus of the adult dog. The ultrastructural distribution of glucose-6-phosphatase activity was investigated in renal glomeruli of adult dogs by electron-microscopic cytochemistry. The enzymatic activity was found mainly in the parietal epithelial cells of Bowman's c...

Wiener, J.O., 1989: Perspectives. FDA scandal: white hats, black hats, gray areas. Faulkner & Gray's Medicine & Health 43(48): Suppl 6 P.-Suppl 6 P.

Starke, W.R., 1972: Bowen's disease of the palm associated with Hodgkin's lymphoma. Cancer 30(5): 1315-1318

Sanaka, T., 2003: Nutritional effect of dialysis therapy. The prognosis of patients with end-stage renal disease has been improved by the recent remarkable advances in medical and engineering technology. However, there are still many unsolved problems in the clinical field. One of the problems is an intr...

D'antonio C., 1989: Italian fauna records 128 131. Bollettino della Societa Entomologica Italiana 121(1): 72-73

Paul Thomas R., 1973: The mechanism of burial diagenetic reactions in argillaceous sediments; 4, Infrared evidence. For part 3, see Yeh, Hsueh-Wen, 17.

Watterson, R.L.; Fowler, I., 1953: Regulative development in lateral halves of chick neural tubes. A row of somites and adjacent nephrotome transplanted into the neurocoele of neural tubes of 17- to 28-somite chick embryos partially or completely separated the tube into lateral halves. In 6 specimens rigid mosaic development of the latter occur...

Johnson, E.K., 2015: The business of care: the moral labour of care workers. Drawing on a case study conducted in a private residential care home, this article examines the emotional labour of care workers in relation to the moral construction of care and the practical experiences of work. An examination of the company&apo...