geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Vascular superoxide production by endothelin-1 requires Src non-receptor protein tyrosine kinase and MAPK activation


, : Vascular superoxide production by endothelin-1 requires Src non-receptor protein tyrosine kinase and MAPK activation. Atherosclerosis 212(1): 78-85

ET-1 induces vascular O(2)(*-) production via activation of NADPH oxidase. We have investigated whether c-Src and MAPKs activation are involved in ET-1-induced vascular oxidative response. At 2 h, ET-1 induced an increase in NADPH oxidase-driven O(2)(*-) production in rat isolated aortic rings, which was completely suppressed in PP2 (c-Src inhibitor)-pretreated rings, whereas PP3 (inactive analogue of PP2) was without effect. ET-1 increased the levels of phospho-c-Src, the active form of c-Src, and the phosphorylation of cortactin, a Src-specific substrate. Both c-Src and cortactin phosphorylation induced by ET-1 were prevented by PP2. The increased expression of p47(phox), the main cytosolic subunit of NADPH oxidase, induced by ET-1 was also prevented by PP2. The increased vascular O(2)(*-) production and p47(phox) up-regulation induced by ET-1 was only inhibited in aortic rings coincubated with the ERK1/2 inhibitor, PD98059; being without effects both the p38 MAPK inhibitor, SB203580, and JNK inhibitor, SP600125. Aortic rings incubation with ET-1 increased the phosphorylation of ERK1/2. This effect was suppressed by coincubation with PP2 showing that this event is down-stream of c-Src activation. In conclusion, ET-1 induces NADPH oxidase-driven O(2)(*-) generation through increase of p47(phox) protein expression. The signalling pathway for this effect involves c-Src activation and ERK1/2 phosphorylation.

US$19.90

PMID: 20553682

DOI: 10.1016/j.atherosclerosis.2010.04.031


Other references

Hadfield, M.G.; Weber, N.E., 1975: Effect of fighting and di phenyl hydantoin on the uptake of tritiated levo norepinephrine in vitro in synaptosomes isolated from retired male breeding mice. The uptake of 3Hnorepinephrine (NE) was significantly altered by fighting and by diphenylhydantoin in synaptosomes isolated from white Swiss male mice retired from breeding activity. The double reciprocal plot of l/net uptake velocity in nmoles/g...

Bian, C.; Zhang, D.; Guo, Q.; Cai, W.; Zhang, J., 2011: Localization and sex-difference of steroid receptor coactivator-1 immunoreactivities in the brain of adult female and male mice. Females and males are different in brain and behaviors. These differences are mediated by steroids and their nuclear receptors which require coactivators to regulate the transcription of target genes. Studies have shown that these coactivators are...

Burke, L.M.B.; Bashir, M.R.; Neville, A.M.; Nelson, R.C.; Jaffe, T.A., 2015: Current opinions on medical radiation: a survey of oncologists regarding radiation exposure and dose reduction in oncology patients. The aim of this study was to evaluate oncologists' opinions about the use of ionizing radiation in medical imaging of oncology patients. An electronic survey was e-mailed to 2,725 oncologists at the top 50 National Cancer Institute-funded can...

Van Coppenolle, F.; Ahidouch, A.; Guilbault, P.; Ouadid, H., 1997: Regulation of endogenous CA-2+ channels by cyclic AMP and cyclic GMP-dependent protein kinases in Pleurodeles oocytes. The effects of cyclic AMP (cAMP) and cyclic GMP (cGMP) on dihydropyridine sensitive Ca-2+ channels were investigated under voltage-clamp in defolliculated Pleurodeles oocytes. Intracellular injection of cAMP or extracellular application of the per...

Born, W.C., 1952: Evaluation and prophylaxis of occupational skin diseases. Zeitschrift für Haut- und Geschlechtskrankheiten 13(8): 245-248

Ionescu M.A.; Weinberg M., 1971: Fauna of the socialist republic of romania insecta vol 11 part 11 diptera asilidae. Fauna Of The Socialist Republic Of Romania Insecta Vol 11 Part 11 Diptera Asilidae 288

Cherry, S.; Jin, E.Jennifer.; Ozel, M.Neset.; Lu, Z.; Agi, E.; Wang, D.; Jung, W-Hung.; Epstein, D.; Meinertzhagen, I.A.; Chan, C-Chiang.; Hiesinger, P.Robin., 2013: Charcot-Marie-Tooth 2B mutations in rab7 cause dosage-dependent neurodegeneration due to partial loss of function. The small GTPase Rab7 is a key regulator of endosomal maturation in eukaryotic cells. Mutations in rab7 are thought to cause the dominant neuropathy Charcot-Marie-Tooth 2B (CMT2B) by a gain-of-function mechanism. Here we show that loss of rab7, bu...

Terryberry, J.W.; Shoenfeld, Y.; Sherer, Y.; Levy, Y.; Fabrizzi, F.; Ahmed, A.; Peter, J.B., 2000: Detection of antibodies to gangliosides and glycolipids in various intravenous immunoglobulin (IVIg) preparations. The aim of this study was to examine the presence of antibodies to GM1 and sulfatide in various IVIg preparations. Five brands of commercially available human IVIg (Sandoglobulin, Isiven, Cytogam, Omrigam and Cutter) were examined and compared. Se...

Parkkinen, J.; Von Bonsdorff Lindeberg, L., 2001: Pharmaceutical preparations. The present invention relates to pharmaceutical compositions of transferrin and to the manufacture of such preparations. The present invention concerns specifically a pharmaceutical composition of apotransferrin, the iron-free form of transferrin,...

Shetty, K.G.; Hetrick, B.A.; Figge, D.A.; Schwab, A.P., 1994: Effects of mycorrhizae and other soil microbes on revegetation of heavy metal contaminated mine spoil. The effects of mycorrhizal fungi and other soil microorganisms on growth of two grasses, Andropogon gerardii Vitm. and Festuca arundinacea Schreb., in heavy metal-contaminated soil and mine tailings were investigated. A. gerardii is highly depende...