+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

VirD: a virion display array for profiling functional membrane proteins

, : VirD: a virion display array for profiling functional membrane proteins. Analytical Chemistry 85(17): 8046-8054

To facilitate high-throughput biochemical analyses of membrane proteins, we have developed a novel display technology in a microarray format. Both single-pass (cluster of differentiation 4, CD4) and multiple-pass (G protein-coupled receptor 77, GPR77) human transmembrane proteins were engineered to be displayed in the membrane envelop of herpes simplex virions. These viruses produce large spherical virions displaying multiple copies of envelop proteins. Our aim was to engineer this virus to express these human proteins during the virus productive cycle and incorporate the human proteins into the virion during the assembly process. Another strategy presented includes engineering a fusion of glycoprotein C (gC), a major constituent of herpes simplex virus type 1 (HSV-1) virions, by hijacking the cis-acting signals to direct incorporation of the chimeric protein into the virion. The expression of the human proteins in infected cells, at the cell surface and in purified virions, is in the correct transmembrane orientation, and the proteins are biochemically functional. Purified virions printed on glass slides form a high-density Virion Display (VirD) Array, and the displayed proteins were demonstrated to retain their native conformations and interactions on the VirD Array judging by similar assays, such as antibody staining, as well as lectin and ligand binding. This method can be readily scaled or tailored for different modalities including a high-content, high-throughput platform for screening ligands and drugs of human membrane proteins.


PMID: 23941274

DOI: 10.1021/ac401795y

Other references

Lopez-Colon, Jose-Ignacio, 1995: Hybalus ameliae Lopez-Colon, 1986 and Hybalus baguenai Lopez-Colon, 1986, two species from the Iberian south. (Coleoptera, Scarabaeoidea, Orphnidae). Hybalus ameliae Lopez-Colon, 1986 and Hybalus baguenai Lopez-Colon, 1986, two species from the Iberian south - Hybalus ameliae (Hybalus ameliae ameliae Lopez-Colon, 1986) and Hybalus baguenai (Hybalus ameliae baguenai Lopez-Colon, 1986) are distin...

Corman, L., 1967: The double in the family-drawing test: its psychopathological significance. L' Evolution Psychiatrique 32(1): 117-147

Chung, I.Yeuk.Wah.; Paetzel, M., 2013: Crystal structures of yellowtail ascites virus VP4 protease: trapping an internal cleavage site trans acyl-enzyme complex in a native Ser/Lys dyad active site. Yellowtail ascites virus (YAV) is an aquabirnavirus that causes ascites in yellowtail, a fish often used in sushi. Segment A of the YAV genome codes for a polyprotein (pVP2-VP4-VP3), where processing by its own VP4 protease yields the capsid prote...

Grimaldi, R.; Acosta, A.-Xavier; Cabral-Oliveira, F.-Cesar; Brites, C.; Galvao-Castro, B., 2005: Study of the CCR5-m303 mutation in three different ethnic groups from Brazil. The main coreceptor gene involved in HIV-1 infection is CCR5 beta chemokine receptor gene for which several mutations have been described, some of which have correlated with HIV-1 infection, acquired immune deficiency syndrome (AIDS), or both. Del...

Bagó, Z.; Bauder, B.; Kolodziejek, J.; Nowotny, N.; Weissenböck, H., 2002: Tickborne encephalitis in a mouflon (Ovis ammon musimon). In April 1994, a 3-year-old male mouflon was found in a moribund condition in a hunting preserve in Burgenland, Austria. It was killed and submitted for pathological examination. The major macroscopic findings were poor nutritional status and seve...

Yamamoto, J.; Nakazawa, D.; Tsukaguchi, H.; Toyoyama, T.; Sato, A.; Nakagaki, T.; Ishikawa, Y.; Shibazaki, S.; Nishio, S.; Atsumi, T., 2013: Case report; A case of familial focal segmental glomerulosclerosis with a mutation in the formin INF2. Nihon Naika Gakkai Zasshi. Journal of the Japanese Society of Internal Medicine 102(5): 1220-1222

McIntyre, C.L.; Manners, J.M., 1996: Strategies for the suppression of peroxidase gene expression in tobacco. I. Designing efficient ribozymes. Five short hammerhead ribozymes (Rzs) were constructed and tested, using a range of in vitro reaction conditions, for catalytic activity against the mRNA encoding the lignin-forming peroxidase (TPX) of tobacco. Although all 5 Rzs were shown to be...

Shahdadpuri, R.; Lambert, D.; Lynch, S.A., 2010: Diagnostic outcome following routine genetics clinic referral for the assessment of global developmental delay. The aim of this study was to ascertain the diagnostic yield following a routine genetics clinic referral for the assessment of global developmental delay. Detailed retrospective review of 119 complete consecutive case notes of patients referred to...

Kittlitz, E., 1982: Breeding of synthetic varieties of broadbeans (Vicia faba L.) Autofertility.1. Vortrage fur Pflanzenzuchtung Arbeitsgemeinschaft Pflanzenzuchtung Gesellschaft fur Pflanzenbauwissenschaften: ) 157-168

Ubeda, F., 2008: Evolution of genomic imprinting with biparental care: implications for Prader-Willi and Angelman syndromes. The term "imprinted gene'' refers to genes whose expression is conditioned by their parental origin. Among theories to unravel the evolution of genomic imprinting, the kinship theory prevails as the most widely accepted, because it...