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Virus-specific effects of TRIM5α(rh) RING domain functions on restriction of retroviruses


, : Virus-specific effects of TRIM5α(rh) RING domain functions on restriction of retroviruses. Journal of Virology 87(13): 7234-7245

The tripartite motif protein TRIM5α restricts particular retrovirus infections by binding to the incoming capsid and inhibiting the early stage of virus infection. The TRIM5α RING domain exhibits E3 ubiquitin ligase activity and assists the higher-order association of TRIM5α dimers, which promotes capsid binding. We characterized a panel of RING domain mutants of the rhesus monkey TRIM5α (TRIM5α(rh)) protein. The RING domain function that significantly contributed to retroviral restriction depended upon the restricted virus. The E3 ubiquitin ligase activity of the RING domain contributes to the potency of HIV-1 restriction. Nonetheless, TRIM5α(rh) mutants without detectable E3 ubiquitin ligase activity still blocked reverse transcription and inhibited HIV-1 infection at a moderate level. When TRIM5α(rh) capsid binding was weakened by substitution with a less efficient B30.2/SPRY domain, the promotion of higher-order association by the RING domain was more important to HIV-1 restriction than its E3 ubiquitin ligase activity. For the restriction of N-tropic murine leukemia virus (N-MLV) and equine infectious anemia virus (EIAV) infection, promotion of higher-order association represented the major contribution of the RING domain. Thus, both identity of the target virus and the B30.2/SPRY domain-mediated affinity for the viral capsid determine the relative contribution of the two known RING domain functions to TRIM5α restriction of retrovirus infection.

US$19.90

PMID: 23637418

DOI: 10.1128/JVI.00620-13


Other references

Barigozzi, C., 1980: An interdisciplinary symposium on the brine shrimp Artemia in the USA. Experientia (Basel)  36.5: 627

Medinskii, G.M.; Ladnyi, I.D.; Bichul, K.G.; Goncharov, A.G.; Kiseleva, V.I.; Bystryi, N.F.; Kudryakova, T.A.; Rudakov, I.M.; Mishchenko, O.A.; Et-Al, 1976: A case of contamination of karst water bearing strata by cholera vibrios. An underground strata bearing sulfide waters apparently was infected with cholera vibrio. Infection occurred due to the absence of a protection zone and intense contamination of the territory above the water-bearing strata. Sources of infection co...

Bellion, B.; Tribuno, C., 1957: Practical aspects of application of betatron to radiotherapy. Minerva Medica 48(23): 963-971

Werner M.S., 1991: Vulcanoseismospeleology; impact of Richter 6.1 temblor upon Malama Cave, Puna District, Hawaii; an insider's view. Geo (super 2) 19(1): 21

Gorissen, M.; de Vrieze, E.; Flik, G.; Huising, M.O., 2011: STAT genes display differential evolutionary rates that correlate with their roles in the endocrine and immune system. We identified orthologues of all mammalian Janus kinase (JAK) and signal transducer and activator of transcription (STAT) genes in teleostean fishes, indicating that these protein families were already largely complete before the teleost tetrapod...

Le, V.P.; Kovacs, A.; Wagenseil, J.E., 2012: Measuring left ventricular pressure in late embryonic and neonatal mice. Blood pressure increases significantly during embryonic and postnatal development in vertebrate animals. In the mouse, blood flow is first detectable around embryonic day (E) 8.5(1). Systolic left ventricular (LV) pressure is 2 mmHg at E9.5 and 11...

Iwaku F., 1971: On the distribution of blood vessels and hepatic ducts in the liver of crab eating monkey macaca irus. Acta Anatomica Nipponica 46(4): 210-223

Taljedal I B., 1974: Mechanisms of insulin release effects of sulfhydryl reagents on islet cyclic amp. Acta Endocrinologica Supplementum 190:

Davies, Ajs, 1986: Evolution and functions of the immune system. Ruminant immune system in health and disease edited by W Ivan Morrison

Conceição, E.M.; Machado, P.P.P.; Vaz, A.Rita.; Pinto-Bastos, A.; Ramalho, S.; Silva, Cátia.; Arrojado, F., 2016: APOLO-Bari, an internet-based program for longitudinal support of bariatric surgery patients: study protocol for a randomized controlled trial. Despite evidence of successful weight loss for bariatric surgery patients, some patients experience considerable weight regain over the long term. Given the strong association between post-surgery health behaviors and outcomes, aftercare intervent...