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WRB and CAML are necessary and sufficient to mediate tail-anchored protein targeting to the ER membrane

, : WRB and CAML are necessary and sufficient to mediate tail-anchored protein targeting to the ER membrane. Plos One 9(1): E85033-E85033

Tail-Anchored (TA) proteins are inserted into the endoplasmic reticulum (ER) membrane of yeast cells via the posttranslational Guided Entry of Tail-Anchored protein (GET) pathway. The key component of this targeting machinery is the ATPase Get3 that docks to the ER membrane by interacting with a receptor complex formed by the proteins Get1 and Get2. A conserved pathway is present in higher eukaryotes and is mediated by TRC40, homolog of Get3, and the recently identified membrane receptors WRB and CAML. Here, we used yeast lacking the GET1 and GET2 genes and substituted them with WRB and CAML. This rescued the growth phenotypes of the GET receptor mutant. We demonstrate that WRB and CAML efficiently recruit Get3 to the ER membrane and promote the targeting of the TA proteins in vivo. Our results show that the membrane spanning segments of CAML are essential to create a functional receptor with WRB and to ensure TA protein membrane insertion. Finally, we determined the binding parameters of TRC40 to the WRB/CAML receptor. We conclude that together, WRB and CAML are not only necessary but also sufficient to create a functional membrane receptor complex for TRC40. The yeast complementation assay can be used to further dissect the structure-function relationship of the WRB/CAML heteromultimer in the absence of endogenous receptor proteins.


PMID: 24392163

DOI: 10.1371/journal.pone.0085033

Other references

Fardon, D., 2003: 3. From the Clinic. Spine Journal 1(1): 79-79

Bamia, C.; Lagiou, P.; Buckland, G.; Grioni, S.; Agnoli, C.; Taylor, A.J.; Dahm, C.C.; Overvad, K.; Olsen, A.; Tjønneland, A.; Cottet, V.; Boutron-Ruault, M-Christine.; Morois, S.; Grote, V.; Teucher, B.; Boeing, H.; Buijsse, B.; Trichopoulos, D.; Adarakis, G.; Tumino, R.; Naccarati, A.; Panico, S.; Palli, D.; Bueno-de-Mesquita, H.Bas.; van Duijnhoven, Fänzel.J.B.; Peeters, P.H.M.; Engeset, D.; Skeie, G.; Lund, E.; Sánchez, M-José.; Barricarte, A.; Huerta, J-Maria.; Quirós, J.Ramón.; Dorro, 2013: Mediterranean diet and colorectal cancer risk: results from a European cohort. The authors investigated the association of adherence to Mediterranean diet with colorectal cancer (CRC) risk in the European Prospective Investigation into Cancer and nutrition study. Adherence to Mediterranean diet was expressed through two 10-u...

Mizutani, S.; Takagi, M., 2013: XCIND as a genetic disease of X-irradiation hypersensitivity and cancer susceptibility. The XCIND syndrome is named after distinct hypersensitivity to ionizing (X-ray) irradiation, cancer susceptibility, immunodeficiency, neurological abnormality, and double-strand DNA breakage. The disorders comprising XCIND syndrome are usually inh...

Kapuścik, J.; Matlakowski, W., 1987: Two contributions to the youthful literary production of Władysław Matlakowski. Archiwum Historii i Filozofii Medycyny 50(1): 65-73

Hawke B.R.; Lucey P.G., 1986: Spectral reflectance study of the Hadley-Appenine (Apollo 15) region. NASA Technical Memorandum: ; Pages 523-525. 1986.

Schrör, K.; Seidel, H., 1988: Blood-vessel wall arachidonate metabolism and its pharmacological modification in a new in vitro assay system. Prostacyclin and thromboxane production was measured in human umbilical cord arteries bathed in clotting human blood and compared with arteries bathed in Krebs buffer or clotting blood without vessels. In comparison with the combined system, vesse...

Šarbort, M.; Řeřucha, Šimon.; Holá, M.; Buchta, Zěk.; Lazar, J., 2016: Self-referenced interferometer for cylindrical surfaces. We present a new interferometric method for shape measurement of hollow cylindrical tubes. We propose a simple and robust self-referenced interferometer where the reference and object waves are represented by the central and peripheral parts, resp...

Thorarinsson Sigurdur, 1939: Vatnajoekull; scientific results of the Swedish-Icelandic investigations 1936-37-38; Chapter VIII, Hoffellsjoekull, its movements and drainage. Geografiska Annaler 3-4: 189-215

Tali V., 1984: Amino acid content of electrophoretically separated protein fractions of legume seeds. Protein fractions were separated by electrophoresis in polyacrylamide gel from the saline solution extract of the seeds of Pisum sativum (cv. Jogeva roheline and cv. Torsdag) and Phaseolus vulgaris (cv. Gruzinskaya pyostraya and cv. Krupnaya sakha...

Cheung, M.; Bao, W.; Behm, D.J.; Brooks, C.A.; Bury, M.J.; Dowdell, S.E.; Eidam, H.S.; Fox, R.M.; Goodman, K.B.; Holt, D.A.; Lee, D.; Roethke, T.J.; Willette, R.N.; Xu, X.; Ye, G.; Thorneloe, K.S., 2017: Discovery of GSK2193874: An Orally Active, Potent, and Selective Blocker of Transient Receptor Potential Vanilloid 4. Transient Receptor Potential Vanilloid 4 (TRPV4) is a member of the Transient Receptor Potential (TRP) superfamily of cation channels. TRPV4 is expressed in the vascular endothelium in the lung and regulates the integrity of the alveolar septal ba...