geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Whole cell screen for inhibitors of pH homeostasis in Mycobacterium tuberculosis


, : Whole cell screen for inhibitors of pH homeostasis in Mycobacterium tuberculosis. Plos One 8(7): E68942-E68942

Bacterial pathogens like Mycobacterium tuberculosis (Mtb) encounter acidic microenvironments in the host and must maintain their acid-base homeostasis to survive. A genetic screen identified two Mtb strains that cannot control intrabacterial pH (pHIB) in an acidic environment; infection with either strain led to severe attenuation in mice. To search for additional proteins that Mtb requires to survive at low pH, we introduced a whole-cell screen for compounds that disrupt pHIB, along with counter-screens that identify ionophores and membrane perturbors. Application of these methods to a natural product library identified four compounds of interest, one of which may inhibit novel pathway(s). This approach yields compounds that may lead to the identification of pathways that allow Mtb to survive in acidic environments, a setting in which Mtb is resistant to most of the drugs currently used to treat tuberculosis.

US$19.90

PMID: 23935911

DOI: 10.1371/journal.pone.0068942


Other references

Martini G.; Flavell R., 1985: The control of nucleolus volume in wheat a genetic study at 3 developmental stages. The major nucleoli in the wheat [Triticum aestivum] cultivar Chinese Spring are formed at the nucleolus organizers (NOR) on chromosomes 1B and 6B. Minor nucleoli are formed from the NOR on chromosomes 5D and 1A. Nucleolar volume is poorly correlat...

Garcia Sainz Luis, 1941: Las fases epiglaciares del Pirineo espanol. Distinguishes three epiglacial phases in the Spanish Pyrenees--Buehlm, Gsnitz, and Danna. Deposits of the Buehlm phase are massif moraines of sand and pebbles located in U-shaped Wuerm valleys. The epiglacial detrital material of the Gsnitz phase...

Chun, Jaesun, 2003: Phenoloxidase activity in hemolymph of Plasmodium-melanizing and nonmelanizing strains of Anopheles gambiae. Phenoloxidase (PO) activity was compared in two strains of the mosquito, Anopheles gambiae, one of which melanotically encapsulates and kills malaria parasites (Plasmodium spp.) and a second which permits normal development of these parasites. The...

Zucker, N.L.; Losh, M.; Bulik, C.M.; LaBar, K.S.; Piven, J.; Pelphrey, K.A., 2007: Anorexia nervosa and autism spectrum disorders: guided investigation of social cognitive endophenotypes. Death by suicide occurs in a disproportionate percentage of individuals with anorexia nervosa (AN), with a standardized mortality ratio indicating a 57-fold greater risk of death from suicide relative to an age-matched cohort. Longitudinal studies...

Richey, D.J., 1962: Water-soluble nitrofuran therapy in pullorum and fowl typhoid in chicks. Day-old chicks were experimentally inoculated with Salmonella pullorum or Salmonella gallinarum. Medication with either of 2 soluble nitrofurans (nitrofurazone and furaltadone) in drinking water, alone and supplemented with furazolidone-medicated...

Chater A.O., 1969: Carex marina m an earlier name for carex amblyorhyncha m. Feddes Repertorium3: 103-106

Legg, A.; Hughes, R., 2011: Reply to Vavrova et al. Clin Chem Lab Med 2011;49:89-92. Clinical Chemistry and Laboratory Medicine 49(7): 1243-1244

Gasparetto, E.L.; Bertholdo, D.B.; Davaus, T.; Marchiori, E.; Escuissato, D.L., 2007: Stenotrophomonas maltophilia pneumonia after bone marrow transplantation: case report with emphasis on the high-resolution CT findings. A 6-year-old female patient who underwent bone marrow transplantation because of Fanconi anaemia presented with fever, dyspnoea and cough 17 days after the procedure. The physical examination revealed diffuse crackles. Chest radiographs demonstrat...

Solà, J.; Morris, G.A.; Clayden, J., 2011: Measuring screw-sense preference in a helical oligomer by comparison of 13C NMR signal separation at slow and fast exchange. While an unequal population of rapidly interconverting left- and right-handed conformers of a helical oligomer can be detected by circular dichroism, precise quantification of a conformer ratio has not previously been achieved. We demonstrate, usi...

Kabir, S.M.H.; Khan, M.S.lar; Begum, S., 1996: Pest insects from two herbaria in Dhaka city. Six species of insect pests including Liposcelis sp., Lepisma saccharina (L.), Lasioderma serricome (F.), Ephestia cautella (Walker), Anthrenus scrophulariae (L.) and Attagenus piceus (Oliv.) were found to infest the herbarium specimens. The most...