+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Zebrafish type XVII collagen: gene structures, expression profiles, and morpholino "knock-down" phenotypes

, : Zebrafish type XVII collagen: gene structures, expression profiles, and morpholino "knock-down" phenotypes. Matrix Biology 29(7): 629-637

The human COL17A1 gene encodes type XVII collagen (also known as the 180-kDa bullous pemphigoid antigen), an integral component of hemidesmosomes, attachment complexes providing integrity to the dermal-epidermal junction. Zebrafish, a useful model system to study skin development, displays fully developed hemidesmosomes at approximately 5 days post-fertilization (dpf). We have identified two COL17A1 orthologues in the zebrafish genome, col17a1a and col17a1b, which are expressed in the skin and the neural system, respectively. The proteins coded by these genes have structural module organizations homologous to the human type XVII collagen. "Knock-down" of the expression of col17a1a with a specific morpholino targeting the 5' UTR of the gene resulted in a blistering phenotype and in perturbations in the basement membrane zone. "Knock-down" of col17a1b expression resulted in ablation or in marked reduction of neuromasts in the lateral line. Thus, zebrafish has two COL17A1 orthologues which may have evolved tissue-specific functions during vertebrate development. Collectively, zebrafish provides a model system to study the molecular aspects of skin development and offers insights into the corresponding human diseases.


PMID: 20670682

DOI: 10.1016/j.matbio.2010.07.002

Other references

Weber, R.; Keerl, R.; Draf, W., 2000: Endonasal endoscopic surgery of maxillary sinus mucoceles after Caldwell-Luc operation. Mucoceles of the maxillary sinus after Caldwell-Luc procedure develop by entrapment of mucosa. Specially mucoceles far laterally were being revised by a second transoral approach. This procedure carries the risk of damaging the infraorbital nerve....

Yoshida T., 1992: International regulatory control of drugs of abuse. The mechanism for the international regulatory control of drugs of abuse under the Single Convention on Narcotic Drugs and the Convention on Psychotropic Substances is outlined. A historical overview of the substances which have been added to the...

Grottola, A.; Forghieri, F.; Meacci, M.; Fabio, A.; Pozzi, L.; Marchegiano, P.; Codeluppi, M.; Morselli, M.; Potenza, L.; Paolini, A.; Coluccio, V.; Luppi, M.; Rumpianesi, F.; Pecorari, M., 2013: Severe pneumonia caused by Legionella pneumophila serogroup 11, Italy. Emerging Infectious Diseases 18(11): 1911-1913

Axelrod, J.D.; Majors, J.; Brandriss, M.C., 1991: Proline-independent binding of PUT3 transcriptional activator protein detected by footprinting in vivo. The PUT3 gene product is a transcriptional activator required for expression of the enzymes of the proline utilization pathway. Using two methods of footprinting in vivo, we have determined that PUT3 protein is poised at the promoters of the genes...

Moreno-Alcázar, A.; Treen, D.; Valiente-Gómez, A.; Sio-Eroles, A.; Pérez, Víctor.; Amann, B.L.; Radua, J., 2017: Efficacy of Eye Movement Desensitization and Reprocessing in Children and Adolescent with Post-traumatic Stress Disorder: A Meta-Analysis of Randomized Controlled Trials. <b>Background:</b> Post-traumatic stress disorder (PTSD) can occur in both adults and children/adolescents. Untreated PTSD can lead to negative long-term mental health conditions such as depression, anxiety, low self-concept, disruptiv...

Sylvain, H.; Arsenault, L., 1994: Participatory approach to patients with fibromyalgia: exploring the nurse's role. L'infirmiere du Quebec 1(6): 36-43

Reise, S.P.; Moore, T.M.; Haviland, M.G., 2011: Bifactor models and rotations: exploring the extent to which multidimensional data yield univocal scale scores. The application of psychological measures often results in item response data that arguably are consistent with both unidimensional (a single common factor) and multidimensional latent structures (typically caused by parcels of items that tap simi...

Bruggeman, E.C.; Garretson, J.T.; Wu, R.; Shi, H.; Xue, B., 2017: Neuronal Dnmt1 deficiency attenuates diet-induced obesity in mice. Aberrant neuronal DNA methylation patterns have been implicated in the promotion of obesity development, but the role of neuronal DNA methyltransferases (Dnmts; enzymes that catalyze DNA methylation) in energy balance remains poorly understood. We...

Beltrame, M.O.; Fugassa, M.H.; Sardella, N.H., 2010: First paleoparasitological results from late Holocene in Patagonian coprolites. These are the first paleoparasitological results from the Late Holocene in Patagonian coprolites. Coprolites collected from layers dated at 3,480 and 2,740 yr before present (B.P.), from the archaeological site Cerro Casa de Piedra, were examined....

Kamp, G., 1986: Phosphat, ein bedeutender Regulator der Glykogenolyse? In vivo Untersuchungen am Schwanzmuskel der Garnele Crangon crangon. Verhandlungen der Deutschen Zoologischen Gesellschaft, 79: 388-389