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bFGF-induced human periodontal ligament fibroblasts proliferation through T-type voltage-dependent calcium channels

, : bFGF-induced human periodontal ligament fibroblasts proliferation through T-type voltage-dependent calcium channels. Acta Odontologica Scandinavica 71(1): 9-14

To test the hypothesis that T-type voltage-dependent calcium channels (T-CaCNs) are involved in basic fibroblast growth factor (bFGF)-induced proliferation of human periodontal ligament fibroblasts (HPLFs). This study examined the expression of the T-type calcium channel sub-units Ca(V) 3.1, Ca(V) 3.2 and Ca(V) 3.3 in HPLFs by real-time PCR. Mibefradil, a T-CaCNs antagonist, was used to block the effect of T-CaCNs and the proliferation of HPLFs was evaluated by the water-soluble tetrazolium (WST) assay. The level of intracellular calcium was measured by laser confocal microscopy. Expression of the three subunits of T-CaCNs in HPLFs was detected, which was strongly up-regulated upon stimulation by bFGF. The proliferation of HPLFs induced by bFGF was decreased significantly by treatment with Mibefradil. This effect was associated with the decreased expression of T-CaCNs and a decreased level of intracellular calcium. Expression of the T-CaCNs affected the proliferation of HPLFs that was induced by bFGF, indicating that T-CaCNs could be important in mediating periodontal ligament (PDL) remodeling.


PMID: 22251131

DOI: 10.3109/00016357.2011.645067

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