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bFGF-mediated redox activation of the PI3K/Akt pathway in retinal photoreceptor cells

, : bFGF-mediated redox activation of the PI3K/Akt pathway in retinal photoreceptor cells. European Journal of Neuroscience 33(4): 632-641

In many retinal diseases, it is the death of photoreceptors that leads to blindness. In previous in vitro and in vivo studies, basic fibroblast growth factor (bFGF) has been shown to increase retinal cell survival. More recently, reactive oxygen species (ROS) have also been shown to promote cell survival, contrary to the traditional view that they are solely destructive molecules. Due to this possible link, we hypothesised that bFGF could stimulate the production of ROS, which in turn stimulates the protein kinase B (Akt) survival pathway. Flow cytometry was used to measure the fluorescence of oxidised dihydrorhodamine, a ROS indicator, in the murine 661W photoreceptor cell line under several different conditions. Expression of cyclooxygenase (Cox) enzymes was evaluated by immunohistochemistry, and the response of photoreceptor cells to exogenous bFGF in the explanted mouse retina was studied by confocal microscopy. Exogenous addition of bFGF to 661W cells resulted in an increase in ROS production that lasted for 24 h. When this ROS production was inhibited, bFGF-induced phosphorylation of Akt was prevented. Through the use of inhibitors and small interfering RNA in the cell line, the source of this production was shown to be Cox and to involve the activation of phospholipases A(2) + C. This pathway may also occur in the mouse retina, as we showed that the retina expressed Cox1&2, and that photoreceptors in explanted retina respond to bFGF by increasing their ROS levels. These results demonstrate that exogenous bFGF can stimulate ROS production through the activation of Cox, and activate the Akt pathway.


PMID: 21219477

DOI: 10.1111/j.1460-9568.2010.07559.x

Other references

Hanks, G.E.; Cassell, M.; Ray, R.N.; Chaplin, H., Jr., 1960: Further modification of the benzidine method for measurement of hemoglobin in plasma : definition of a new range of normal values. A method is described for the collection of blood samples with the minimum of artifactual haemolysis. Normal plasma so obtained contained too little Hb for estimation by available methods so the benzidine method of Crosby and Furth (Blood, 1956, 1...

Cao, X-Yuan.; Jia, Z-Fang.; Cao, D-Hui.; Kong, F.; Jin, M-Shan.; Suo, J.; Jiang, J., 2015: DNMT3a rs1550117 polymorphism association with increased risk of Helicobacter pylori infection. DNA methyltransferase-3a (DNMT3a) plays significant roles in embryogenesis and the generation of aberrant methylation in carcinogenesis. This study aimed to investigate associations between single nucleotide polymorphisms (SNPs) of the DNMT3a gene...

Areche, C.; Theoduloz, C.; Yáñez, T.; Souza-Brito, A.R.M.; Barbastefano, Víctor.; de Paula, Débora.; Ferreira, A.L.; Schmeda-Hirschmann, G.; Rodríguez, J.A., 2008: Gastroprotective activity of ferruginol in mice and rats: effects on gastric secretion, endogenous prostaglandins and non-protein sulfhydryls. The gastroprotective mechanism of the natural diterpene ferruginol was assessed in mice and rats. The involvement of gastric prostaglandins (PGE(2)), reduced glutathione, nitric oxide or capsaicin receptors was evaluated in mice either treated or...

Brooten, D.; Youngblut, J.M.; Hannan, J.; Caicedo, C.; Roche, R.; Malkawi, F., 2015: Infant and child deaths: Parent concerns about subsequent pregnancies. Examine parents' concerns about subsequent pregnancies after experiencing an infant or child death (newborn to 18 years). Thirty-nine semistructured parent (white, black, Hispanic) interviews 7 and 13 months post infant/child death conducted...

Kogarko L.N., 1986: Inhomogeneity of the upper mantle and composition of primary magmas of oceanic islands. Soviet Geology and Geophysics 27(7): 62-67

Miroslavov E.A., 1971: The structure of trichome chloroplasts of the leaf epidermis in verbascum thapsus d. Tsitologiya 13(1): 108-111

Jueppner H.; Abou Samra A B.; Uneno S.; Potts J.T.Jr; Segre G.V., 1989: Characterization of the biological properties of three photoderivatized analogs of tyrosine 36 parathyroid hormone related peptide 1 36 amide pthrp 1 36. Acta Endocrinologica Supplementum 120(1): 250-251

Roszak, W., 1973: Effect of application of nitrogen fertilizer on yield and value as a preceding crop of perennial legumes and their mixture with grasses. 1. Application of nitrogen to red clover and lucerne grown alone or in mixtures with grasses. In field trials at Chylice in 1962-6, red clover and lucerne were grown alone or with 7.5 or 20% each of timothy and tall oatgrass and were given 0-160 kg N/ha. Application of N to red clover or lucerne grown alone had no effect on yields but incr...

Hoddinott, J.; Karachiwalla, N.I.; Ledlie, N.A.; Roy, S., 2016: Adolescent girls' infant and young child nutrition knowledge levels and sources differ among rural and urban samples in Bangladesh. In many low-income countries, girls marry early and have children very soon after marriage. Although conveying infant and young child nutrition (IYCN) knowledge to adolescent girls in time is important to ensure the well-being of their children, l...

Lopez Perez, A.; Casanova O.E.; Chacon, L.A.; Paz P.M.; Guerrero, J.R., 1991: Agronomic effectiveness of several phosphate rocks of Tachira state with maize (Zea mays L.) as test crop. In pot experiments with maize as the test crop, the efficiency of rock phosphate from the first and second layers of the Monte Fresco deposits (MF-1 and MF-2, resp.) and from the San Joaquin de Navay deposits (SJN) in Venezuela was in the followin...