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1,3-Propanediol production by new recombinant Escherichia coli containing genes from pathogenic bacteria

, : 1,3-Propanediol production by new recombinant Escherichia coli containing genes from pathogenic bacteria. Microbiological Research 171: 1-7

1,3-Propanediol (1,3-PDO) is an organic compound, which is a valuable intermediate product, widely used as a monomer for synthesizing biodegradable polymers, increasing their strength; as well as an ingredient of textile, cosmetic and medical products. 1,3-PDO is mostly synthesized chemically. Global companies have developed technologies for 1,3-PDO synthesis from petroleum products such as acrolein and ethylene oxide. A potentially viable alternative is offered by biotechnological processes using microorganisms capable of synthesizing 1,3-PDO from renewable substrates (waste glycerol, a by-product of biofuel production, or glucose). In the present study, genes from Citrobacter freundii and Klebsiella pneumoniae were introduced into Escherichia coli bacteria to enable the synthesis of 1,3-PDO from waste glycerol. These strains belong to the best 1,3-PDO producers, but they are pathogenic, which restricts their application in industrial processes. The present study involved the construction of two gene expression constructs, containing a total of six heterologous glycerol catabolism pathway genes from C. freundii ATCC 8090 and K. pneumoniae ATCC 700721. Heterologous genes encoding glycerol dehydratase (dhaBCE) and the glycerol dehydratase reactivation factor (dhaF, dhaG) from C. freundii and gene encoding 1,3-PDO oxidoreductase (dhaT) from K. pneumoniae were expressed in E. coli under the control of the T7lac promoter. An RT-PCR analysis and overexpression confirmed that 1,3-PDO synthesis pathway genes were expressed on the RNA and protein levels. In batch fermentation, recombinant E. coli bacteria used 32.6gl(-1) of glycerol to produce 10.6 gl(-1) of 1,3-PDO, attaining the efficiency of 0.4 (mol₁,₃-PDO molglycerol(-1)). The recombinant E. coli created is capable of metabolizing glycerol to produce 1,3-PDO, and the efficiency achieved provides a significant research potential of the bacterium. In the face of shortage of fossil fuel supplies and climate warming there is an increasing industrial need to exchange the chemical way of chemicals synthesis for biotechnological - more ecological manner. The 1,3-PDO production from glycerol is an desirable alternative to the traditional production from non-renewable resources. This work is a part of project, which opens a way to development of innovative "green chemistry" and new perspectives to chemical industry.


PMID: 25644946

DOI: 10.1016/j.micres.2014.12.007

Other references

Fujieda, S.; Suzuki, S.; Sunaga, H.; Yamamoto, H.; Seki, M.; Sugimoto, H.; Saito, H., 2000: Production of interferon-gamma by tonsillar mononuclear cells in IgA nephropathy patients. Much evidence, both in vivo and in vitro, suggests that patients with IgA nephropathy (IgAN) have enhanced IgA production. We hypothesized that Haemophilus parainfluenzae (HP) in the tonsil plays an important role in the IgA production of IgAN pat...

Jenkins, Z.A.; Hååg, P.G.; Johansson, H.E., 2001: Human eIF5A2 on chromosome 3q25-q27 is a phylogenetically conserved vertebrate variant of eukaryotic translation initiation factor 5A with tissue-specific expression. Eukaryotic translation initiation factor 5A (eIF5A) is an essential protein tightly linked to cellular polyamine homeostasis. It receives the unique spermidine-derived posttranslational modification hypusine that is necessary for eIF5A's bioc...

Simpson, J.E., 1959: The influence of auditory stimulation on aniseikonic perception. II. and III. Business school women and college women. Journal of Genetic Psychology 94(2): 263-266

Anonymous, 1982: Rice production policy of Nigeria. Newsletter International Rice Commission 31(1): 17-19

Redmond, M.L.; Dong, F.; Frazier, L.M., 2015: Does the extended parallel process model fear appeal theory explain fears and barriers to prenatal physical activity?. Few studies have looked at the impact of fear on exercise behavior during pregnancy using a fear appeal theory. It is beneficial to understand how women receive the message of safe exercise during pregnancy and whether established guidelines have...

Lapiere, S., 1953: Multiple zoniform hemiatrophy. Archives Belges de Dermatologie et de Syphiligraphie 9(3): 197-200

Mahan, D.E.; Copeland, R.L., 1978: A method using toluene-2,4-diisocyanate and glutaraldehyde to stabilize and conjugate antigens to erythrocytes for use in passive hemagglutination tests. Erythrocytes were stabilized and activated for conjugation by simultaneous treatment with toluene-2,4-diisocyanate and glutaraldehyde. Following conjugation with an antigen, these cells were suitable for use in passive hemagglutination tests and c...

Kleinsteinberg, K.; Gries, T.; Jockenhoevel, S.; Morticelli, L.; Thomas, D., 2014: Track d: cellular, tissue and bioengineering. Biomedizinische Technik. Biomedical Engineering 59 Suppl 1: S263-S325

Dawson J.B.; James D.; Paslick C.; Halliday A., 1995: Thermal anomaly in the upper mantle beneath a propagating continental rift; evidence from mantle xenoliths from the Labait Volcano, northern Tanzania. Proceedings of the International Kimberlite Conference: ges 124-125. 1995.

Nascimento, W.M.; West, S.H., 1998: Priming and seed orientation affect seedcoat adherence and seedling development of muskmelon transplants. The effects of seed priming and seed orientation on seedcoat adherence and seedling development of containerized muskmelon transplants were investigated. Seeds of muskmelon 'Top Net SR' were primed for 6 days in darkness at 25 degreeC in...