+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

A highly sensitive and facile graphene oxide-based nucleic acid probe: Label-free detection of telomerase activity in cancer patient's urine using AIEgens

, : A highly sensitive and facile graphene oxide-based nucleic acid probe: Label-free detection of telomerase activity in cancer patient's urine using AIEgens. Biosensors & Bioelectronics 89(Pt 1): 417-421

NlmCategory="UNASSIGNED">Molecular beacon (MB)-based sensing platforms that consist of a fluorogen-quencher pair play an important role in medical and biological researches. However, the synthesis of both fluorogen and quencher in the nucleic acid probes will increase the burden of organic synthesis works and induce the difficulties for precisely controlling the relative distance between fluorogen and quencher, which may lead to false-positive and false-negative results. In this work, initially we report a single labeled MB (FAM-MB, with carboxyfluorescein as fluorogen and without quencher) thus simplifies MBs with the aid of graphene oxide (GO) to detect telomerase activity. To further simplify this structure, namely label-free strategy, we design a facile, sensitive and selective platform using a label-free beacon (AIE-MB, without fluorogen and quencher), based on aggregation-induced emission fluorogen (silole-R). Upon the addition of telomerase, AIE-MB induced comb-like DNA structure leads to high aggregation of silole-R and thus exhibits strong fluorescence emission. By exploitation of this, we can detect telomerase with superior sensitivity and demonstrate their applications in bladder cancer diagnosis. Compared to single-labeled FAM-MB based telomerase activity assay, the label-free AIE-MB induced method could perform the sensitive detection with high signal-to-background ratio.


PMID: 27184557

DOI: 10.1016/j.bios.2016.05.035

Other references

Hayat; Shafie, 1997: Reversible Severe Mitral Regurgitation Provoked by Reversible Myocardial Ischemia Ñ Abolition by Coronary Artery Stenting. Journal of Invasive Cardiology 9(5): 352-354

Allen M.F.; Allen E.B.; Helm D.J.; Rincon E., 1992: Mycorrhizal dynamics of restored and successional habitats comparison of temperature and tropical biomes. Bulletin of the Ecological Society of America 73(2 SUPPL): 95-96

Lee, R.C.; Hrmova, M.; Burton, R.A.; Lahnstein, J.; Fincher, G.B., 2003: Bifunctional family 3 glycoside hydrolases from barley with alpha-L-arabinofuranosidase and beta-D-xylosidase activity. Characterization, primary structures COOH-terminal processing. An alpha-L-arabinofuranosidase and a beta-D-xylosidase, designated ARA-I and XYL, respectively, have been purified about 1,000-fold from extracts of 5-day-old barley (Hordeum vulgare L.) seedlings using ammonium sulfate fractional precipitation, i...

Crichlow, T.V., 1955: Discussion on mastoid radiology. Journal of Laryngology and Otology 69(8): 531-550

Needham, D.M.; Cohen, J.A.; Barrett, A.M., 1947: The mechanism of damage to the bone marrow in systemic poisoning with mustard gas. After intraven. injn. of a lethal dose of mustard gas (H) the anerobic glycolysis and the respiration of rabbit bone marrow were inhibited. This inhibition was progressive, being just obvious after 4 hr. and very marked after 24 hr., and it ran pa...

Well M.; Albers W.; Fischer A.; Jones P.G.; Schmutzler R., 1992: Reactions of fluorophosphoranes with trimethylsilyl trimethylsilyloxyacetate structures nmr spectroscopic studies and x ray investigations of mono and spirocyclic acyloxyphosphoranes. The reaction of the tetrafluorophosporanes RPF4 (R = Me, Ph) with trimethylsilyl (trimethylsilyloxy)acetate (1) yields the corresponding monocyclic fluoro(acyloxy)phosphoranes 2 and 3 by cleavage of the Si-O bond and elimination of (CH3)3SiF. 2 an...

Rosen, BR., 1988: Biogeographic patterns a perceptual overview. Unknown

Takeuchi, M.; Tatsumi, Y.; Kitaichi, K.; Baba, K.; Suzuki, R.; Shibata, E.; Takagi, K.; Miyamoto, K-ichi.; Hasegawa, T.; Takagi, K., 2002: Selective phosphodiesterase type 4 inhibitors reduce the prolonged survival of eosinophils stimulated by granulocyte-macrophage colony-stimulating factor. It is well known that bronchial asthma is defined as chronic eosinophilic inflammation of the respiratory tract and that as one of the various types of inflammatory cells, eosinophils induce the airway inflammation of chronic asthma. Granulocyte-m...

Klosner, D., 1970: Our experiences with the subtalaric arthrodesis. Monatsschrift für Unfallheilkunde, Versicherungs-, Versorgungs- und Verkehrsmedizin 73(12): 551-559

Sharashidze, L.K.; Sturua, N.S., 1970: On the role of ribonucleoproteins in proliferation and maturation processes in malignant mouse fibroblasts. Folia Histochemica et Cytochemica 8(3): 281-285