+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

A proposed harmonized LPS molecular-subtyping scheme for Cronobacter species

, : A proposed harmonized LPS molecular-subtyping scheme for Cronobacter species. Food Microbiology 50: 38-43

Cronobacter are opportunistic pathogens, which cause infections in all age groups. To aid the characterization of Cronobacter in foods and environments a harmonized LPS identification scheme for molecular serotyping is needed. To this end, we studied 409 Cronobacter isolates representing the seven Cronobacter species using two previously reported molecular serotyping schemes, described here as Mullane-Jarvis (M-J) and Sun schemes. PCR analysis revealed many overlapping results that were obtained when independently applying the two serotyping schemes. There were complete agreements between the two PCR schemes for Cronobacter sakazakii (Csak) O:1, Csak O:3, and Csak O:7 serotypes. However, only thirty-five of 41 Csak O:4 strains, identified using the M-J scheme, were PCR-positive with the Sun scheme primers. Also the Sun scheme Csak O:5 primers failed to identify this serotype in any of the C. sakazakii strains tested, but did recognize seven Cronobacter turicensis strains, which were identified as Ctur O:3 using the M-J scheme. Similarly, the Sun scheme Csak O:6 primers recognized 30 Cronobacter malonaticus O:2 strains identified with the M-J scheme, but failed to identify this serotype in any C. sakazakii strain investigated. In this report, these findings are summarized and a harmonized molecular-serotyping scheme is proposed which is predicated on the correct identification of Cronobacter species, prior to serotype determination. In summary, fourteen serotypes were identified using the combined protocol, which consists of Csak O:1-O:4, and Csak O:7; Cmal O:1-O:2; Cdub O:1-O:2, Cmuy O:1-O:2, Cuni O:1, as well as Ctur O:1 and Ctur O:3.


PMID: 25998813

DOI: 10.1016/

Other references

Tjuvajev, J.G.; Chen, S.H.; Joshi, R.; Balatoni, J.; Finn, R.; Larson, S.M.; Woo, S.L.; Blasberg, R.G., 1999: Imaging adenoviral-mediated HSV1-tk gene expression in an animal model of metastatic colon carcinoma to the liver. Journal of Nuclear Medicine 40(5 SUPPL ): 227P, May

White, E., 1992: Setting a good example. Journal of the Royal Society of Health 112(5): 255-255

Edye, L.A.; Clarke, M.A.; McDonald Lewis, C., 1994: Universal NIR calibrations for the sugar industry. Over the past 4 sugarcane seasons and the past 3 sugarbeet campaigns, SPRI researchers collected NIR spectra of raw materials, process streams and products (plus data from conventional analyses) from several cane and beet sugar factories. The prim...

Bao, H.; Zhang, H.; Liu, G.; Li, Y.; Cai, W., 2017: Nanoscaled Amorphous TiO 2 Hollow Spheres: TiCl 4 Liquid Droplet-Based Hydrolysis Fabrication and Strong Hollow Structure-Enhanced Surface-Enhanced Raman Scattering Effects. A very simple route is developed for fast fabrication of nanosized amorphous titanium dioxide (TiO<sub>2</sub>) hollow spheres (THPs) just via dropping the pure four titanium chloride (TiCl<sub>4</sub>) liquid droplets into...

Sokol, R.Z., 2001: Infertility in men with cystic fibrosis. The majority of men with cystic fibrosis (CF) have associated congenital bilateral absence of the vas deferens (CBVAD). This congenital defect results in the absence of the anatomic ducts through which spermatozoa pass from the testes to the ureth...

Li, M.; Liu, P.; Adzic, R.R., 2012: Platinum Monolayer Electrocatalysts for Anodic Oxidation of Alcohols. The slow, incomplete oxidation of methanol and ethanol on platinum-based anodes as well as the high price and limited reserves of Pt has hampered the practical application of direct alcohol fuel cells. We describe the electrocatalysts consisting o...

Tang, T.Q.; Duan, S.F.; Zhang, Q.P., 1983: Studies on some parameters of tricuspid valve echocardiogram in the early diagnosis of chronic cor pulmonale. Acta Academiae Medicinae Wuhan 3(2): 117-122

Ngouonkadi, E.Bertrand.Megam.; Fotsin, H.Bertrand.; Nono, M.Kabong.; Fotso, P.Herve.Louodop., 2017: Erratum to: Noise effects on robust synchronization of a small pacemaker neuronal ensemble via nonlinear controller: electronic circuit design. [This corrects the article DOI: 10.1007/s11571-016-9393-1.].

Tomé, A.M.; Teixeira, A.R.; Figueiredo, N.; Georgieva, P.; Santos, I.M.; Lang, E., 2011: Clustering evoked potential signals using subspace methods. This work proposes a clustering technique to analyze evoked potential signals. The proposed method uses an orthogonal subspace model to enhance the single-trial signals of a session and simultaneously a subspace measure to group the trials into cl...

Delone, N.L.; Kozlov, V.A., 1964: Effect of B-mercaptopropvlamine in lowering the number of chromosomal rearrangements in Tradescantia paludosa mlcrospores as a result of gamma-irradiation. The number of chromosomal rearrangements in the experimental and control experiments was substantially different: in the irradiated specimens that were not protected by [beta]-mercaptopropylamine it was 5.49 [plus or minus] 0.535. In the irradiate...