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Acanthopanax trifoliatus inhibits lipopolysaccharide-induced inflammatory response in vitro and in vivo


, : Acanthopanax trifoliatus inhibits lipopolysaccharide-induced inflammatory response in vitro and in vivo. Kaohsiung Journal of Medical Sciences 31(10): 499-509

Acanthopanax trifoliatus is a well-known herb that is used for the treatment of bruising, neuralgia, impotence, and gout in Taiwan. This herb exhibits multifunctional activities, including anticancer, anti-inflammation, and antioxidant effects. This paper investigated the in vitro and in vivo anti-inflammatory effect of A. trifoliatus. High-performance liquid chromatography analysis established the fingerprint chromatogram of the ethyl acetate fraction of A. trifoliatus (EAAT). The anti-inflammatory effect of EAAT was detected using lipopolysaccharide (LPS) stimulation of the mouse macrophage cell line RAW264.7 in vitro and LPS-induced lung injury in vivo. The effects of EAAT on LPS-induced production of inflammatory mediators in RAW264.7 murine macrophages and the mouse model were measured using enzyme-linked immunosorbent assay and Western blot. EAAT attenuated the production of LPS-induced nitric oxide (NO), tumor necrosis factor-alpha, interleukin-1β (IL-1β), and IL-6 in vitro and in vivo. Pretreatment with EAAT markedly reduced LPS-induced histological alterations in lung tissues. Furthermore, EAAT significantly reduced the number of total cells and protein concentration levels in the bronchoalveolar lavage fluid. Western blotting test results revealed that EAAT blocked protein expression of inducible NO synthase, cyclooxygenase-2, phosphorylation of Nuclear factor-kappa-B Inhibitor alpha (IκB-α) protein, and mitogen-activated protein kinases in LPS-stimulated RAW264.7 cells as well as LPS-induced lung injury. This study suggests that A. trifoliatus may be a potential therapeutic candidate for the treatment of inflammatory diseases.

US$19.90

PMID: 26520688

DOI: 10.1016/j.kjms.2015.07.007


Other references

Malan, E.; Puglionisi, A., 1949: Retromediastinal prevertebral space. Minerva Chirurgica 4(24): 679-683

Kim, Y.Soo.; Lee, S.Won.; Yeom, J.A.; Yoon, C.Hyo.; Baik, S.Kug., 2016: Angiographic findings of in-stent intimal hyperplasia after stent-assisted coil embolization: are they permanent findings?. Stent-assisted coil embolization for the treatment of intracranial aneurysms has been used widely. This study aimed to investigate the effect of stent implantation in the nonatherosclerotic parent artery with cerebral aneurysms. The authors evalua...

Goebel, J.A.; Isipradit, P.; Hanson, J.M., 2000: Comparison of manual whole-body and head-on body rotation with rotary chair testing - practical and theoretical considerations. Claussen, Claus-Frenz [Author], Haid, Claus-Toni [Author], Hofferberth, Bernhard [Author] International Congress Series; Equilibrium research, clinical equilibriometry and modern treatment 212

Edler, L.; Hagelthorn, M., 1990: Identification of the causative organism of a DSP-outbreak on the Swedish west coast. Unknown

Sheloumova, A.; Protod'iakonov, O., 1933: Role of Azotobacter in the N nutrition of higher plants under conditions prevailing in Leningrad. Addition of chalk to podzolized sandy soil in pots increased by 25-100% the yield of tobacco and by 35% the yield of maize. Addition of Azotobacter and chalk + Azotobacter increased by 8 and 122% the yield of tobacco, and by 10 and 72% the yield o...

Havermans, T.; Vreys, M.; Proesmans, M.; D.B.eck, C., 2006: Assessment of agreement between parents and children on health-related quality of life in children with cystic fibrosis. Child: Care, Health and Development 32(1): 1-7

Li, S.Cheng., 2013: The difficulty of protein structure alignment under the RMSD. Protein structure alignment is often modeled as the largest common point set (LCP) problem based on the Root Mean Square Deviation (RMSD), a measure commonly used to evaluate structural similarity. In the problem, each residue is represented by th...

Zhang, J.; Cheng, R.; Liang, J.; Ni, C.; Li, M.; Yao, Z., 2016: Report of a child with sporadic familial progressive hyper- and hypopigmentation caused by a novel KITLG mutation. British Journal of Dermatology: -

Jones, A.E.; Taylor, T.; Wright, G.W.; Kort, H.I.; Massey, J.B.; Nagy, Z.P., 2003: Fertilization, embryo development, and chromosomal status after intracytoplasmic sperm injection of metaphase I human oocytes. Fertility & Sterility 80(Suppl 3): S119-S120, September

Wolfe, M.Ys, 1967: Whiteheads philosophy of organism. Analytic Philosophy 8(1): 6-7