+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

An LC-MS/MS method for multi-mycotoxin quantification in cow milk

, : An LC-MS/MS method for multi-mycotoxin quantification in cow milk. Food Chemistry 218(): 378-385

NlmCategory="UNASSIGNED">The simultaneous quantification of 15 mycotoxins in cow milk by liquid chromatography-mass spectrometry, is presented. Extraction was performed with acidified acetonitrile, followed by a cleanup step with sodium acetate. During validation limits of detection (LOD) and quantification, linearity, precision, accuracy, recovery, matrix effect, and stability were studied. LOD values were between 0.02 and 10.14ng/mL for aflatoxins M1, B1, B2, G1, G2, ochratoxins A and B, HT-2 and T-2 toxins, deepoxy-deoxynivalenol, zearalenone, sterigmatocystin and fumonisins B1, B2 and B3. Recovery values were between 82.6 and 94.4% for all the mycotoxins, except for fumonisins. The recovery values for fumonisins were between 42.1% and 64.6%. Matrix effect, between 25.5 and 96.8%, appeared for all of the mycotoxins, especially for deepoxy-deoxynivalenol, zearalenone and sterigmatocystin. The validated method achieves the quantification of those mycotoxins of major concern and mycotoxins that are not frequently studied in milk, such as fumonisins, sterigmatocystin or ochratoxin B.


PMID: 27719924

DOI: 10.1016/j.foodchem.2016.09.101

Other references

Kafer E., 1981: Mutagen sensitivities and mutator effects of methyl methanesulfonate sensitive mutants in neurospora crassa. Seven mus (mutagen-sensitive) mutants of N. crassa, which are more sensitive to the toxic effects of MMS (methyl methanesulfonate) than wild-type, were investigated for cross-sensitivities to other mutagens and inhibitors. These mutants were mappe...

Kuhn, H.; Kuhn, C., 1978: Evolution of a genetic code simulated with the computer. A simple self organizing model system of molecules is considered. It is demonstrated by a computer simulation that a genetic code of 16 elements (amino acids) can gradually be formed by such a system in the course of many generations. By a number...

Thiella, M.; Saiani, L.; Ambrosi, E., 2016: The post-basic courses for nurses offered by Italian Universities. Assistenza Infermieristica E Ricerca 35(3): 128-131

Poulsen, L.K., 2002: Prediction of allergenicity of gene-modified foods by serum-based testing. On the basis of applying the IFBC/ILSI decision tree in a number of cases, a refinement of the scheme is suggested. Large differences in allergenic potential may be obtained by altering the route of administration of an allergen. Because an inhala...

Saha, N.; Hawkins, B.R.; Wong, H.B., 1975: Distribution of ABO and Rhesus blood groups in G6PD deficient Chinese and Malay newborns. The distribution of ABO blood groups was studied in 459 Chinese and 65 Malay newborns with deficiency of G6PD, and in 1181 Chinese and 535 Malay newborns with normal levels of the enzyme. Similarly, the distribution of Rhesus blood groups was stud...

Whittle, M.J., 1995: Prenatal screening Ultrasound. Whittle, M J , Connor, J M Prenatal diagnosis in obstetric practice, Second edition 30-33

Weinreb, O.; Mandel, S.; Bar-Am, O.; Amit, T.; Youdim, M., 2011: Iron-chelating backbone coupled with monoamine oxidase inhibitory moiety as novel pluripotential therapeutic agents for Alzheimer's disease: a tribute to Moussa Youdim. It is for these authors a great privilege to dedicate this review article to Moussa Youdim, who is one of the most imperative pharmacologists and pioneer investigators in the search and development of novel therapeutics for neurodegenerative disea...

Seredenko M.M.; Minyailenko T.D.; Velikanov E.B.; Karyakin A.V.; Rezaev A.A., 1991: The effect of inhalation of air mixture with hydrogen sulphide containing natural gas on lipid peroxidation and glycolysis in rats. It is shown that inhalation of gas with increased hydrogen sulphide content causes an inhibition of lipid peroxidation, an increase of blood lactate concentration and lactate/pyruvate ratio. Inhalation of hydrogen sulphide leads to inhibition of l...

Stapleton, J.T.; Raina, V.; Winokur, P.L.; Walters, K.; Klinzman, D.; Rosen, E.; McLinden, J.H., 1993: Antigenic and immunogenic properties of recombinant hepatitis A virus 14S and 70S subviral particles. Hepatitis A virus (HAV) has an immunodominant neutralization antigenic site. By using a panel of monoclonal antibodies targeted against the HAV neutralization antigenic site, it was shown that three epitopes within this site are present on 14S sub...

Moore, D.; Griffin, D.H.Reviewer, 1999: Fungal morphogenesis (book review). The Quarterly Review of Biology 74(3): 9-50