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Antibiotics and iron-limiting conditions and their effect on the production and composition of outer membrane vesicles secreted from clinical isolates of extraintestinal pathogenic E. coli


, : Antibiotics and iron-limiting conditions and their effect on the production and composition of outer membrane vesicles secreted from clinical isolates of extraintestinal pathogenic E. coli. Proteomics. Clinical Applications (): -

The focus of this study was to characterize the effect of clinically relevant stress-inducing conditions on the production and composition of proinflammatory outer membrane vesicles (OMVs) produced from ST131 extraintestinal pathogenic Escherichia coli (ExPEC) clinical isolates. A label-free method (relative normalized spectral index quantification, SINQ) was used to identify changes in the respective OMV proteomes following exposure of the ExPEC strains to antibiotics and low iron. Nanoparticle tracking analysis was used to quantify changes in abundance and size of OMVs produced by the gentamicin-resistant (GenR) and gentamicin-sensitive (GenS) ExPEC strains. Up to a 13.1-fold increase in abundance of particles were detected when the gentamicin-sensitive strain was exposed to a range of gentamicin concentrations. In contrast, no increase was observed for the gentamicin-resistant strain. Iron-limiting conditions had minimal effect on OMV production for either strain. Marked changes in the OMV proteome were observed for both strains including increases in Hsp100/Clp proteins, ATP-dependent ClpP protease, and regulatory proteins. These data provide information on changes in the composition of OMV particles derived from ExPEC strains generated in response to clinically relevant conditions. We show that the levels of the proinflammatory OMVs increase for gentamicin-sensitive ExPEC exposed to the antibiotic.

US$19.90

PMID: 27666736

DOI: 10.1002/prca.201600091


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Tereanu, C.; Bettega, S.; Regalia, E.; Mazza, R.; Greco, M.; Lozza, L.; Saita, L.; Borreani, C.; Quattrocolo, A., 2014: Voluntary mediation in healthcare disputes: the experience of the "Fondazione IRCCS Istituto Nazionale dei Tumori" in Milan (Italy). The "Fondazione IRCCS Istituto Nazionale dei Tumori" ( National Tumour Institute) in Milan (Italy) offers mediation services aimed at restoring communication between patients and healthcare professionals, when their relationship has been...

Wang, C-Xia.; Huang, L-Song.; Hou, L-Bing.; Jiang, L.; Yan, Z-Ting.; Wang, Y-Lun.; Chen, Z-Liang., 2010: Antitumor effects of polysorbate-80 coated gemcitabine polybutylcyanoacrylate nanoparticles in vitro and its pharmacodynamics in vivo on C6 glioma cells of a brain tumor model. Polybutylcyanoacrylate (PBCA) nanoparticles coated with polysorbate-80 have been extensively studied for delivery of drugs into the animal models; however, 1% polysorbate-80 coated gemcitabine PBCA nanoparticles (GCTB-PBCA-NPs) remain unknown. In...

Bloom, F., 1943: A Cytological Study of the Tubular Epithelium in Acute and Chronic Canine Bright's Disease with Especial Reference to the Mitochondria. A cytological study of the kidneys of dogs with spontaneous acute and chronic Bright's disease indicates specific alterations of the mitochondrial elements in the tubular epithelium associated with the regressive, progressive and regenerative...

Horak J., 1971: Western tatra mountain geo biocenosis of the upper forest and krummholz boundaries. Prirodovedne Prace Ustavu Ceskoslovenske Akademie Ved v Brne 5(5): 3-47

Herold, M.; Kabacoff, O.; Cahn, J., 1961: Research of the cumulative effects comparing d-tubocurarine, toxiferine, and diallyl-nor-toxiferine. Repeated administration of a dose not producing atony, at more and more extended time intervals in proportion to the first injection shows that a cumulative effect can be obtained with toxiferine even after a delay of 180 minutes; with diallyl-nor...

Guzdek, A.; Stalinska, K., 1992: Glycated proteins as inducers of acute phase cytokines. Folia Histochemica et Cytobiologica 30(4): 167-169

Liubenov, I.; Buleva, R., 1980: Chemical control of weeds in fru. Selskostopanska nauka = Agricultural science8(1): 71-75

Smith B.L.; Mcdaniel R.; Skotko D.; Owen W., 1989: Color preference and discrimination in the wild turkey. Two wild turkeys were tested to determine if they had a preference for and could discriminate between certain stimuli. The turkeys were found to prefer hues around the wavelength of 510 nm followed by 620 nm. Brightness was also varied; however, i...

Herrera Vazquez, I.; Alvarado Alvarez, R.; Arechiga, H., 2000: A comprehensive morpho-physiological analysis of the X organ-sinus gland system of the crayfish. The main neurosecretory system of crustaceans is a set of 100-200 neuron somata located in the X-organ of the medulla terminalis, in the eyestalk, sending their axons distally to the sinus gland, a neurohaemal organ. In the present study, we did a...

Weintraub, L.R., 1972: Do remissions in acute leukemia depend upon "cell kill"?. Archives of Internal Medicine 129(3): 498-499