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Antibody fragments for stabilization and crystallization of G protein-coupled receptors and their signaling complexes


, : Antibody fragments for stabilization and crystallization of G protein-coupled receptors and their signaling complexes. Methods in Enzymology 557: 247-258

G protein-coupled receptors (GPCRs) are one of the key players in extracellular signal recognition and their subsequent communications with cellular signaling machinery. Crystallization and high-resolution structure determination of GPCRs has been one of the major advances in the area of GPCR biology over the last 7-8 years. There have primarily been three approaches to GPCR crystallization till date. These are fusion protein strategy, thermostabilization, and antibody fragment-mediated crystallization. Of these, antibody fragment-mediated crystallization has not only provided the first breakthrough in structure determination of a non-rhodopsin GPCR but it has also assisted in obtaining structures of fully active conformations of GPCRs. Antibody fragment approach has also been crucial in obtaining structural information on GPCR signaling complexes. Here, we highlight the specific examples of GPCR crystal structures that have utilized antibody fragments for promoting crystallogenesis and structure solution. We also discuss emerging powerful technologies such as the nanobody technology and the synthetic phage display libraries in the context of GPCR crystallization and underline how these tools are likely to propel key GPCR structural studies in future.

US$19.90

PMID: 25950968

DOI: 10.1016/bs.mie.2015.01.010


Other references

Saarinen, P., 1953: A statistical study of the interrelations between certain mineral constituents of the blood of dairy cows. Single blood samples were taken from 330 cows between the 20th and 252nd days of lactation and receiving different amounts of dietary Ca, P, Na and Cl. Blood levels of these elements and total excess of cations were estimated. Coefficients of part...

Malhotra, K.; Modak, A.; Nangia, S.; Daman, T.H.; Gunsel, U.; Robinson, V.L.; Mokranjac, D.; May, E.R.; Alder, N.N., 2017: Cardiolipin mediates membrane and channel interactions of the mitochondrial TIM23 protein import complex receptor Tim50. The phospholipid cardiolipin mediates the functional interactions of proteins that reside within energy-conserving biological membranes. However, the molecular basis by which this lipid performs this essential cellular role is not well understood....

Salloum, R.G.; Asfar, T.; Maziak, W., 2016: Toward a Regulatory Framework for the Waterpipe. Waterpipe smoking has been dramatically increasing among youth worldwide and in the United States. Despite its general association with misperceptions of reduced harm, evidence suggests this is a harmful and dependence-inducing tobacco use method...

Tepel, M.; Schlotmann, R.; Barenbrock, M.; Kisters, K.; Klaus, T.; Spieker, C.; Walter, M.; Meyer, C.; Bretzel, R.G.; Zidek, W., 1995: Lymphocytic Na(+)-H+ exchange increases after an oral glucose challenge. The effects of oral glucose challenge on plasma glucose concentration, plasma insulin concentration, arterial blood pressure, cytosolic pH (pHi), cytosolic free Na+ concentration ([Na+]i), and cellular Na(+)-H+ exchange activity were investigated...

Marques, J.; Gonçalves, Jão.; Oliveira, Cáudia.; Favero-Longo, S.E.; Paz-Bermúdez, G.; Almeida, R.; Prieto, B., 2016: On the dual nature of lichen-induced rock surface weathering in contrasting micro-environments. NlmCategory="UNASSIGNED">Contradictory evidence from biogeomorphological studies has increased the debate on the extent of lichen contribution to differential rock surface weathering in both natural and cultural settings. This study,...

Csaba, G.; Lantos, T.; Torok, O., 1972: Basic proteins and stainability in connection with the cell individuality cell contact phenomena. Cytobiologie, 6(1): 29-34

Farah, R.; Bleier, J.; Gilbey, P.; Khamisy-Farah, R., 2016: Common Laboratory Parameters for Differentiating Between Community-Acquired and Healthcare-Associated Pneumonia. The correct diagnosis of healthcare-associated pneumonia (HCAP) as opposed to community-acquired pneumonia is essential for the selection of a correct empirical antimicrobial approach, reserving the broad-spectrum or highly potent antimicrobial th...

Wu, X.; Chen, H.; Sun, J.; Peng, Y.; Liang, Y.; Wang, G.; Wu, J.; Zhang, P., 2010: Development and validation of a liquid chromatography-mass spectrometry method for the determination of verticinone in rat plasma and its application to pharmacokinetic study. We have developed and validated a sensitive liquid chromatography-electrospray ionization-mass spectrometric (LC-ESI-MS) method for the quantification of verticinone, a major active constituent from Fritillaria hupehensis Hsiao et KC Hsia., in rat...

Pafko, P.; Kabát, J., 1996: Reoperation after gastroesophageal reflux surgery. In a group of 19 patients reoperated after a primary operation on account of gastrooesophageal reflux the authors demonstrate that the reason for reoperation can be either a relapse of the reflux or also other complaints (most frequently dysphagia...

Sill V., 1974: The significance of the beta receptor system for the euler liljestrand mechanism. Verhandlungen Der Deutschen Gesellschaft Fuer Innere Medizin: 00-903