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Anticancer bioactive peptide-3 inhibits human gastric cancer growth by targeting miR-338-5p

, : Anticancer bioactive peptide-3 inhibits human gastric cancer growth by targeting miR-338-5p. Cell & Bioscience 6(): 53-53

Cancer incidence and mortality have been increasing in China, making cancer the leading cause of death since 2010 and a major public health concern in the country. Cancer stem cells have been studied in relation to the treatment of different malignancies, including gastric cancer. Anticancer bioactive peptide-3 (ACBP-3) can induce the apoptosis of gastric cancer stem cells (GCSCs) and reduce their tumorigenicity. In the present study, for the first time, we used a miRNA microarray and bioinformatics analysis to identify differentially expressed miRNAs in ACBP-3-treated GCSCs and GCSC-derived tumors in a xenograft model and functionally verified the identified miRNAs. miR-338-5p was selected based on its significant upregulation by ACBP-3 both in cultured GCSCs and in tumor tissues. miR-338-5p was downregulated in GCSCs compared with normal gastric epithelial cells, and the ectopic restoration of miR-338-5p expression in GCSCs inhibited cell proliferation and induced apoptosis, which correlated with the upregulation of the pro-apoptotic Bcl-2 proteins BAK and BIM. We also found that ACBP-3-treated GCSCs could respond to lower effective doses of cisplatin (DDP) or 5-fluorouracil (5-FU), possibly because ACBP-3 induced the expression of miR-338-5p and the BAK and BIM proteins and promoted GCSC apoptosis. Our data indicate that miR-338-5p is part of an important pathway for the inhibition of human gastric cancer stem cell proliferation by ACBP-3 combined with chemotherapeutics. ACBP-3 could suppress GCSC proliferation and lower the required effective dose of cisplatin or 5-fluorouracil. Therefore, this study provides not only further evidence for the remarkable anti-tumor effect of ACBP-3 but also a possible new approach for the development of GCSC-targeting therapies.


PMID: 27688872

DOI: 10.1186/s13578-016-0112-8

Other references

Yoon, S.I.; Park, C.J.; Nah, W.H.; Gye, M.C., 2008: Expression of occludin in testis and epididymis of wild rabbits, Lepus sinensis coreanus. Tight junctions (TJs) in inter-Sertoli junctional areas and epididymal epithelia build up the blood-testis barrier (BTB) and the blood-epididymal barrier (BEB), respectively. In this study, the expression of occludin, an integral member of the TJs...

Funck-Brentano, C.; Lancar, R.; Hansen, S.; Hohnloser, S., H.; Vanoli, E., 2001: Predictors of medical events and of their competitive interactions in the Cardiac Insufficiency Bisoprolol Study 2 (CIBIS-2). Aims: Predictive factors for medical events and interactions between events were not reported in the Cardiac Insufficiency Bisoprolol Study 2 (CIBIS-2). We examined the interactions among death, permanent treatment withdrawals, nonlethal cardiovas...

Yager J.D.Jr; Yager R., 1980: Promotion of hepato carcinogenesis by oral contraceptive steroids. Proceedings of the American Association for Cancer Research & American Society of Clinical Oncology 21: 76

Sokhansanj, S.; Barber, E.M., 1982: Solar collector/storage system for swine barns in a cold Canadian climate. A semi-solid concrete-block wall 700 mm from the south face of the farrowing room was equipped to take a solar collector of 2.2 X 18 m. The 9.6 X 15.8 X 2.4 m test barn had 18 farrowing crates; one 500 mm and three 200 mm high-speed direct-drive p...

Geiger D.R.; Saunders M.A., 1969: Path parameters of a translocating sugar beet d leaf. Plant Physiology (Rockville) 44(SUPPL): 22

Moreno, C.; Kabat, E.A., 1969: Studies on human antibodies. 8. Properties and association constants of human antibodies to blood group A substance purified with insoluble specific adsorbents and fractionally fluted with mono- and oligosaccharide. Human antibodies to blood group A substance were purified by absorption on columns of insoluble polyleucyl hog blood group A + H substance and eluted first with N-acetylgalactosamine and then with an A active reduced pentasaccharide AR(L)0.52. The...

Mahan, D.C., 1991: Efficacy of initial postweaning diet and supplemental coconut oil or soybean oil for weanling swine. Two experiments involving a total of 581 crossbred pigs weaned at 23 +/- 2 d of age evaluated 1) the efficacy of two diets that contained milk products during the initial 14 d postweaning and 2) the effect of coconut oil or soybean oil additions t...

Malaty, A.; Sabharwal, J.; Lirette, L.Smallwood.; Chaiban, G.; Eissa, H.; Tolba, R., 2014: How to assess a new patient for a multidisciplinary chronic pain rehabilitation program: a review article. Chronic pain is a debilitating condition that affects people all over the world. To effectively treat chronic pain patients, assignment to patient-centered functional restoration and psychological pain rehabilitation programs at an early stage is...

Lebedev, O.V.; Sokolov, I.N., 1976: Rapid determination of radionuclide activity in the human body. Meditsinskaia Radiologiia 21(11): 3-8

Tang, S-Chun.; Chiu, Y-Hua.; Jeng, J-Shing., 2004: Skin biopsy in cerebral autosomal dominant arteriopathy with subcortical infarct and leukoencephalopathy. Acta Neurologica Taiwanica 13(2): 97-98