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Assessment of two multilocus sequence typing (MLST) schemes available for Streptococcus mutans


, : Assessment of two multilocus sequence typing (MLST) schemes available for Streptococcus mutans. Archives of Oral Biology 60(12): 1769-1776

Two multilocus sequencing typing (MLST) schemes are currently available for Streptococcus mutans. The first, introduced by Nakano et al. in 2007, consists of 8 conserved housekeeping genes. The second, introduced in 2010 by Do et al., includes 6 housekeeping genes and 2 putative virulence genes. The purpose of the current study was to compare the two MLST schemes for use in validating repetitive extragenic palindromic polymerase chain reaction (rep-PCR) genotypes. Thirty-three S. mutans isolates, representing the 11 most commonly occurring rep-PCR genotype groups, were selected for MLST. MLST was performed with SYBR Green™ PCR with published primers for both MLST schemes. Amplicons were purified, sequenced, and data checked against the www.PubMLST.org database for allelic and sequence type (ST) assignment. Discriminatory power, congruence, and convenience criteria were evaluated. Concatenated sequences for each scheme were analyzed using MEGA to generate phylogenetic trees using minimum evolution with bootstrap. No significant difference in discriminatory power was observed between the two MLST schemes for S. mutans. Clonal clusters were consistent for both schemes. Overall, MLST demonstrated marginally greater discriminatory power than rep-PCR; however all methods were found to be congruent. New alleles and ST are reported for each scheme and added to the PubMLST database. Clonality, supported by both methods and rep-PCR, indicates S. mutans genotypes are shared between unrelated subjects. Both Nakano and Do schemes demonstrates similar genotype discrimination for S. mutans isolates suggesting each are well designed and may be used to verify rep-PCR genotypes.

US$19.90

PMID: 26439181

DOI: 10.1016/j.archoralbio.2015.09.012


Other references

Reynolds, J.C.; Del Vecchio, S.; Sakahara, H.; Lora, M.E.; Carrasquillo, J.A.; Neumann, R.D.; Larson, S.M., 1989: Anti-murine antibody response to mouse monoclonal antibodies: clinical findings and implications. Many patients given murine monoclonal antibody become immune to these proteins. Important factors in the development of immunity are (1) the patient's ability to become immune and (2) whether whole immunoglobulin or Fab fragment was used. Cir...

Bour R., 1981: Systematic survey of the malagasy endemic genus pyxis reptilia chelonia. The main stages in the knowledge of the endemic Malagasy genus Pyxis Bell, 1827 are presented. Numerous features that P. arachnoides Bell, 1827 and P. planicauda (Grandidier, 1867) have in common (color pattern and morphology; skull and shell oste...

Fujii, S.; Ziatdinov, M.; Ohtsuka, M.; Kusakabe, K.; Kiguchi, M.; Enoki, T., 2014: Role of edge geometry and chemistry in the electronic properties of graphene nanostructures. The geometry and chemistry of graphene nanostructures significantly affects their electronic properties. Despite a large number of experimental and theoretical studies dealing with the geometrical shape-dependent electronic properties of graphene...

Scott, D., 2014: A practical technique for calcium and phosphorus estimations. Canadian Journal of Medical Technology 9(1): 10-10

Tismer, R.; Mödder, U., 1984: Sonographic detection and the clinical significance of the left retroaortal renal vein. A retro-aortal left renal vein can be expected in 12.2% of the patients, as anatomical examinations show; in 8,9% of the cases, this presents as a circum -aortal venous ring, whereas in 3.3% it occurs as an isolated V. renalis retroaortalis sin. T...

Edwards Jones, E.S., 1997: The River Valleys Project: a participatory approach to integrated catchment planning and management in Scotland. Legislation and institutional structure have restricted opportunities for the integrated planning and management of Scotland's water resources. There is growing recognition that a more holistic and integrated approach to river management may...

Holder, Helmut, 1950: Uber gestreifte Belemniten aus dem jungeren Lias. Descr. of specimens of Belemnites acuarius macer. B. tricanaliculatus, and B. lagenae-formis.

Redding, A.M.; Mukhopadhyay, A.; Joyner, D.C.; Hazen, T.C.; Keasling, J.D., 2006: Study of nitrate stress in Desulfovibrio vulgaris Hildenborough using iTRAQ proteomics. The response of Desulfovibrio vulgaris Hildenborough (DvH), a sulphate-reducing bacterium, to nitrate stress was examined using quantitative proteomic analysis. DvH was stressed with 105 mM sodium nitrate (NaNO<sub>3</sub>), a level th...

Luimes, Jb, 1984: Good soil disinfection with correct methods and circumstances. Vakblad voor de bloemisterij 39(14): 56-57

Gutgesell, H.P.; Massaro, T.A.; Kron, I.L., 1994: The arterial switch operation for transposition of the great arteries in a consortium of university hospitals. American Journal of Cardiology 74(9): 959-960