geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Astragalus polysaccharide attenuates rat experimental colitis by inducing regulatory T cells in intestinal Peyer's patches


, : Astragalus polysaccharide attenuates rat experimental colitis by inducing regulatory T cells in intestinal Peyer's patches. World Journal of Gastroenterology 22(11): 3175-3185

To explore probable mechanism underlying the therapeutic effect of Astragalus polysaccharide (APS) against experimental colitis. Thirty-two Sprague-Dawley rats were randomly divided into four groups. Colitis was induced with 2, 4, 6-trinitrobenzene sulfonic acid (TNBS). The rats with colitis were treated with 400 mg/kg of APS for 7 d. The therapeutic effect was evaluated by colonic weight, weight index of the colon, colonic length, and macroscopic and histological scores. The levels of regulatory T (Treg) cells in Peyer's patches were measured by flow cytometry, and cytokines in colonic tissue homogenates were analyzed using enzyme-linked immunosorbent assay. The expression of related orphan receptor-γt (ROR-γt), IL-23 and STAT-5a was measured by Western blot. After 7-d treatment with APS, the weight index of the colon, colonic weight, macroscopical and histological scores were decreased, while the colonic length was increased compared with the model group. The expression of interleukin (IL)-2, IL-6, IL-17, IL-23 and ROR-γt in the colonic tissues was down-regulated, but Treg cells in Peyer's patches, TGF-β and STAT5a in the colonic tissues were up-regulated. APS effectively ameliorates TNBS-induced experimental colitis in rats, probably through restoring the number of Treg cells, and inhibiting IL-17 levels in Peyer's patches.

US$19.90

PMID: 27003994

DOI: 10.3748/wjg.v22.i11.3175


Other references

Sister Mary James, 1965: The Purchasing Picture. The Need To Read. Hospital Management 100: 108-108

Tsai, Y.L.ong; Liu, J.; Garcia, J.E.; Katz, E.; Compton, G.; Baramki, T.A., 1997: Establishment of an optimal hypo-osmotic swelling test by examining single spermatozoa in four different hyposmotic solutions. in order to find an optimal hypo-osmotic swelling test (HOST) and to identify viable sperm cells from patients with asthenozoospermia for intracytoplasmic sperm injection (ICSI), we tested single motile and non-motile spermatozoa in four hypo-osmo...

Holz, F., 1982: Automated direct photometric determination of methionine and cyst(e)ine content of seed proteins and its application as a fast analytical method in screening field beans. Zeitschrift fur Pflanzenzuchtung = Journal of plant breeding8(2): 103-117

A.S.haily, I.A.; Musleh, R.M.; Tahir, N.M.; Farag, F., 1984: Mycotoxins and fungal contents of corn stored under different storing conditions. Maize grains stored under different conditions in Babylon province during Jan. and May and in Abo-Ghraib during Feb. and Apr. showed considerable variations in moisture content depending on time and location of sampling. Fusarium was the dominant...

Ogoshi S.; Okamura T., 1974: Selection of cadaveric donor. Iwasaki, Yoji (Ed ) Cadaveric Renal Transplantation Xi+287p Illus Igaku Shoin Ltd : Tokyo, Japan (Dist in U S By International Scholarly Book Services, Inc , Portland, Oreg ) (1975)

Reese, E.S., 1964: Ethology of marine zoology. Oceanography and Marine Biology Annual Review, 2: 455-488

Rader, C.P.; Baumann, B.; Sterner, T.; Rolf, O.; Hendrich, C.; Schütze, N.; Jakob, F., 1999: TNF-alpha secretion by human macrophage-like cells in response to wear particles and its modification by drugs. Biomedizinische Technik. Biomedical Engineering 44(5): 135-141

O'Leary, R.; Davies, C.D., 1981: Clara D. Davies. New England Journal of Medicine 305(4): 220-220

Cacciatore, L.; D.M.rco, F.; Cerini, R.; D.R.tis, F., 1977: Free amino acids in plasma during experimental infection of mice with the MHV-3 strain of mouse hepatitis virus. The concentrations of total free amino acids, single free amino acids, urea, and ammonia were determined in plasma of mice during experimental infection with the MHV-3 strain of mouse hepatitis virus. Analysis of free amino acids was done by ion-e...

Skakun, N.P., 1966: A manometric method of studying cholespasmatic and cholespasmolytic properties of drugs Pharmacology and toxicology. Dogs with a chronic Schiff fistula of the gall bladder (without ligation of the common bile duct) were placed in a stand and the opening of the fistula was connected hermetically through a 3-way stopcock with the outlet joint of a water manometer...