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Atomic force microscopy monitoring of temperature dependence of cytochrome BM3 oligomeric state


, : Atomic force microscopy monitoring of temperature dependence of cytochrome BM3 oligomeric state. Biofizika 60(1): 80-87

The change in temperature is one of the factors affecting the activity of enzymes. In this work thermal denaturation and aggregation of cytochrome P450 BM3 were studied by atomic force microscopy. To determine specific temperature transitions the fluorescence analysis was used. In the low melting temperature range, 10-33 degrees C, a decrease in the fluorescence intensity of aromatic residues was observed with an increase in the fluorescence intensity of flavin groups. Protein melting in this range indicated three narrow S-shaped cooperative transitions at temperatures 16, 22 and 29 degrees C. Atomic force microscopy analysis in this temperature range showed that the shape of BM3 molecules remained globular in the form of compact objects (heights h < 7 nm, lateral dimensions d < 50 nm), but protein oligomeric state changed. The first two transitions were accompanied by a decrease in the degree of oligomerization and the third one was accompanied by its increase.

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PMID: 25868344


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Karki, D., 1998: The contextual web of fertility control: a case study of Chisang Village. The paper examines how various village and family/individual level factors have triggered measurable changes in reproductive attitudes and behaviour in Chisang, a mainly migrant village in the Eastern Terai of Nepal. Fertility change is occurring...

Cerqueira, M.D.; Lawrence, A., 2001: Nuclear cardiology update. This article focuses on the following areas of myocardial perfusion imaging: radiotracer and protocol options, pharmacologic stress agents, and protocols and functional assessment with ECG-gated single photon emission CT.

Lucius, R.; Prod'Hon, J.; Kern, A.; Hébrard, G.; Diesfeld, H.J., 1987: Antibody responses in forest and savanna onchocerciasis in Ivory Coast. Three foci of onchocerciasis transmission (two forest and one savanna, respectively) in the Republic of Ivory Coast were chosen for a comparative analysis of Onchocerca volvulus antigens and of patients' antibody responses. Clear differences...

Loughlin, M.; Bluhm, R.; Buetow, S.; Borgerson, K.; Fuller, J., 2017: Reasoning, evidence, and clinical decision-making: The great debate moves forward. When the editorial to the first philosophy thematic edition of this journal was published in 2010, critical questioning of underlying assumptions, regarding such crucial issues as clinical decision making, practical reasoning, and the nature of ev...

Park, S-Jin.; Sohn, H-Jin.; Hong, S-Kwon.; Shin, G-Su., 2009: Influence of atmospheric fluorine plasma treatment on thermal and dielectric properties of polyimide film. Plasma treatment of polyimide surfaces not only causes structural modification during the plasma exposure, but also leaves active sites on the surfaces that are subject to post-reaction. In this work, the effects of atmospheric fluorine plasma tre...

Jongkees, L.B.; Oosterveld, W.J., 1973: Sinusoidal optokinetic stimulation. The use of a subtractor for the pendulum-swing test. Orl; Journal for Oto-Rhino-Laryngology and Its Related Specialties 35(3): 171-177

Freedman, A.N.; Sansbury, L.B.; Figg, W.D.; Potosky, A.L.; Weiss Smith, S.R.; Khoury, M.J.; Nelson, S.A.; Weinshilboum, R.M.; Ratain, M.J.; McLeod, H.L.; Epstein, R.S.; Ginsburg, G.S.; Schilsky, R.L.; Liu, G.; Flockhart, D.A.; Ulrich, C.M.; Davis, R.L.; Lesko, L.J.; Zineh, I.; Randhawa, G.; Ambrosone, C.B.; Relling, M.V.; Rothman, N.; Xie, H.; Spitz, M.R.; Ballard-Barbash, R.; Doroshow, J.H.; Minasian, L.M., 2010: Cancer pharmacogenomics and pharmacoepidemiology: setting a research agenda to accelerate translation. Recent advances in genomic research have demonstrated a substantial role for genomic factors in predicting response to cancer therapies. Researchers in the fields of cancer pharmacogenomics and pharmacoepidemiology seek to understand why individua...

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