geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Biological and molecular characterization of classical swine fever challenge virus from India


, : Biological and molecular characterization of classical swine fever challenge virus from India. Veterinary World 8(3): 330-335

The aim of this study was biological and molecular characterization of classical swine fever (CSF) challenge virus from India. CSF challenge virus maintained at Division of Biological standardization was experimentally infected to two seronegative piglets. The biological characterization was done by clinical sign and symptoms along with postmortem findings. For molecular characterization 5'-nontranslated region, E2 and NS5B regions were amplified by reverse transcription polymerase chain reaction and sequenced. The sequences were compared with that of reference strains and the local field isolates to establish a phylogenetic relation. The virus produced symptoms of acute disease in the piglets with typical post-mortem lesions. Phylogenetic analysis of the three regions showed that the current Indian CSF Challenge virus is having maximum similarity with the BresciaX strain (USA) and Madhya Pradesh isolate (India) and is belonging to subgroup 1.2 under Group 1. Based on biological and molecular characterization of CSF challenge virus from India is described as a highly virulent virus belonging to subgroup 1.2 under Group 1 along with some field isolates from India and Brescia strain.

US$19.90

PMID: 27047093

DOI: 10.14202/vetworld.2015.330-335


Other references

Rousseau, Mj, 1973: To produce while profiting: A vital necessity!. Technique laitiere: 28 (778) 9

Bankier, J.D.H.; Kennedy, R.S.; Lees, A.W.; Macleod, H.M.; Horne, N.W., 1953: A pharmacological trial of para-ethylsulfonyl benzaldehyde thiosemicarbazone in humans. Dosages of Berculon B of 1-2 mg./kg. were followed by gastro-intestinal disturbances, progressive anemia, liver dysfunction, malaise, depression and lassitude, exanthemata, and conjunctival irritation. The avg. blood concns. of the drug increased...

Radzyński, T.; Lusakowski, A.; Swiątek, K.; Story, T., 2009: The influence of microscopic disorder on electron paramagnetic resonance spectra of Eu(2+) ions in Pb(1-x)Ge(x)Te. In mixed crystals, because of the different ionic radii of cations or anions and the randomness in the placement of ions of different kinds, the crystal lattice is locally deformed. Such local deformations have significant influence on the ground...

Iiyama Jean Toshimichi, 1965: The influence of anions on the exchange equilibria of Na-K ions in alkalic feldspars at 600 degrees C under a pressure of 1000 bars. Ion-exchange reactions between Na and K have been studied for synthetic albite and orthoclase and alkaline solutions of various salts (carbonate, sulphate, chloride, bromide, and iodide) at 600 degrees C and a pressure of 1000 bars. The solid phas...

Dhenain, M.; Volk, A.; Picq, J.-Luc; Perret, M.; Boller, F.; Michot, J.-Luc, 1997: Age dependence of the T2-weighted MRI signal in brain structures of a prosimian primate (Microcebus murinus). Mouse lemurs (Microcebus murinus) are prosimian primates described to be convenient models of brain aging. We observed very high correlations between the T2-weighted magnetic resonance imaging (MRI) signal decrease and the natural logarithm of age...

Clegg, S.; Pruckler, J.; Purcell, B.K., 1985: Complementation analyses of recombinant plasmids encoding type 1 fimbriae of members of the family Enterobacteriaceae. Insertion mutants of recombinant plasmids encoding type 1 fimbriae of four genera of enteric bacteria were used to detect genetic complementation. After transformation by pairs of plasmids, double transformants were screened for their ability to e...

Valyaev, B., 1966: Eliminating patches of Cuscuta. In the Kalinin region forage crops and pastures are subject to infestation by Cuscuta spp. Hand lances on 20-m extension pipes were attached to sprayers for spot treatment. MCPA at 12 kg/ha and nitraphen 5% in 450 1. spray/ha were effective.

Borger, M.A., 2015: Minimally invasive rapid deployment Edwards Intuity aortic valve implantation. Annals of Cardiothoracic Surgery 4(2): 193-195

Völler, H., 2004: Risk-adjusted prevention post-MI. A recent clinical registry with 96 participating hospitals specialized in cardiological. rehabilitation provided data from 47324 patients. Registry data showed a significant reduction of risk factors over time. The incidence of systolic hypertensi...

Shaner, D.M.lefyt, T.A.derson, P., 1985: Herbicide resistant maize through cell culture selection. Monograph British Crop Protection Council: 2) 45-50