+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Carbon dots from tryptophan doped glucose for peroxynitrite sensing

, : Carbon dots from tryptophan doped glucose for peroxynitrite sensing. Analytica Chimica Acta 852: 174-180

Tryptophan doped carbon dots (Trp-CD) were microwave synthesized. The optimum conditions of synthesizing of the Trp-CD were established by response surface multivariate optimization methodologies and were the following: 2.5 g of glucose and 300 mg of tryptophan diluted in 15 mL of water exposed for 5 min to a microwave radiation of 700 W. Trp-CD have an average size of 20 nm, were fluorescent with a quantum yield of 12.4% and the presence of peroxynitrite anion (ONOO(-)) provokes quenching of the fluorescence. The evaluated analytical methodology for ONOO(-) detection shows a linear response range from 5 to 25 μM with a limit of detection of 1.5 μM and quantification of 4.9 μM. The capability of the ONOO(-) quantification was evaluated in standard solutions and in fortified serum samples.


PMID: 25441895

DOI: 10.1016/j.aca.2014.08.050

Other references

Bennett, J.E.; Ahrendt, K.M., 1981: Achieving economies of scale through shared ancillary and support services. Topics in Health Care Financing 7(3): 25-34

Fisher, W.L.; Rahel, F.J., 2004: Geographic information systems in fisheries. Summarizes a growing body of information on applications of geographic information systems (GIS) in fisheries research and management.

Sargon, M.F.; Celik, H.Hamdi.; Kapakin, S.; Basar, R.; Aksit, M.Dogan., 2004: Quantitative analysis of myelinated axons of commissural fibers in the cat brain. There are limited data related to the number of commissural axons found in various species. Although the corpus callosum has been investigated in cat, there are no data on the number of its myelinated axons. Additionally, the number of myelinated...

Smyk, M.M., 1964: Effect of thyrotcKicosis on the resistance of erythrocytes to the effect of hemolysis. Investigations showed that in thyro-toxicosis in human patients and in experimental rabbits the resistance of the erythrocytes to hemolysis was reduced by 10-11 p. c. The erythrogram showed that its peak was higher and displaced to the left as com...

Rechmann, P.; Liou, S.W.; Rechmann, B.M.T.; Featherstone, J.D.B., 2015: Performance of a light fluorescence device for the detection of microbial plaque and gingival inflammation. The hypothesis to be tested was that using the SOPROCARE system in fluorescence perio-mode allows scoring of microbial plaque that is comparable to the Turesky modification of the Quigley Hein plaque index (T-QH) and scoring of gingival inflammati...

Bath-Hextall, F.; Delamere, F.M.; Williams, H.C., 2008: Dietary exclusions for established atopic eczema. Atopic eczema (AE) is a non-infective chronic inflammatory skin disease characterised by an itchy red rash. To assess the effects of dietary exclusions for the treatment of established atopic eczema. We searched The Cochrane Skin Group Specialised...

Baier, K.A.; Meyer, J.A.; O'brien, B.J.; Flora Ginter, G.; Morris, T.L.; Patterson, T.; Bryan, C.F., 1994: Solubilized HLA class I antigen enzyme-linked immunoassay to identify HLA-typing reagents. The objective of this study was to evaluate the use of sHLA in a solid-phase EIA as a rapid and sensitive way to identify potential IgG HLA class-I-typing reagents. To evaluate the efficacy of the sHLA EIA, we used the assay to screen 259 HLA-A, -...

Biedermann, B.; Eberhardt, W.; Reichelt, W., 1994: GABA uptake into isolated retinal Muller glial cells of the guinea-pig detected electrophysiologically. In order to investigate GABA uptake into Muller glial cells electrophysiologically, we used the whole-cell voltage-clamp technique. Since Muller cells were insensitive to application of muscimol and baclofen, the expression of GABA-A and GABA-B re...

Mikadze E.L.; Kharebava I.G.; Gelashvili N.Sh; Tumanishvili G.D., 1990: Proliferative activity of cells and the characteristics of the structural organization of the subependymal layer of the lateral ventricle dorso lateral wall in puppies during early postnatal ontogeny. Imcm thick sections of the subependymal layer of the lateral ventricle dorso-lateral wall of puppies at different ages were studied. It has been revealed that the organization of the epithelium covering the lumen of the ventricle, the width and mi...

Friedrich, L., 1957: Late gastroscopic picture of resected stomach. Orvosi Hetilap 98(1-4): 39-41