+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Caveolin-1-dependent and -independent uPAR signaling pathways contribute to ganglioside GT1b induced early apoptosis in A549 lung cancer cells

, : Caveolin-1-dependent and -independent uPAR signaling pathways contribute to ganglioside GT1b induced early apoptosis in A549 lung cancer cells. American Journal of Cancer Research 4(6): 801-810

Urokinase receptor interacts with α5β1-integrin and enhances cancer cell proliferation and metastasis. Activation of α5β1-integrin requires caveolin-1 and is regulated by uPAR, which upregulates persistently the activated ERK necessary for tumor growth. In this study, we show that the ganglioside GT1b induces proapoptotic signaling through two uPAR-ERK signaling pathways in A549 lung cancer cells. GT1b downregulated the expression of α5β1 integrin, caveolin-1, fibronectin, FAK, and ERK, whereas GT1b upregulated the expression of p53 and uPAR, suggesting GT1b mediated depletion of caveolin-1 in uPAR-expressing A549 cells also disrupts uPAR/integrin complexes, resulting in downregulation of fibronectin-α5β1-integrin-ERK signaling. Following p53 siRNA treatment, FAK and ERK expression was recovered, meaning the presence of reentry uPAR-FAK-ERK signaling pathway. These findings reveal that GT1b is involved in both caveolin-1-dependent uPAR-α5β1-integrin-ERK signaling and caveolin-1-independent uPAR-FAK-ERK signaling. These results suggest a novel function of GT1b as a dual regulator of ERK by modulating caveolin-1 and p53.


PMID: 25520869

Other references

O'Connor, W.J., 2001: Porphyria cutanea tarda and HIV: two cases associated with hepatitis C. Hepatitis C virus (HCV) is a probable etiologic factor for the development of porphyria cutanea tarda (PCT), a photosensitive skin disease causing blistering, skin fragility, milia, and scarring. In a review of the literature, the hepatitis C stat...

Tanyeli, C.; Iyigun, C., 2003: Manganese(III) acetate based oxidation of substituted alpha'-position on cyclic alpha,beta-unsaturated ketones. Selective oxidation of the tertiary alpha'-position on various 2-cyclopentenone, 2-cyclohexenone and aromatic ketone derivatives with manganese(III) acetate is described.

Karandaev, S.E., 1958: Development of normal anatomy in the USSR during the period of Soviet regime. Vestnik Akademii Meditsinskikh Nauk SSSR 13(1): 42-51

Fortune, J.E.; Kito, S.; Wandji, S.A.; Srsen, V., 2000: Activation of bovine and baboon primordial follicles in vitro. Mammalian ovaries contain a large pool of non-growing, primordial follicles. The ability to initiate growth of this pool of resting follicles in vitro and to maintain follicular growth to a stage when the oocyte could be matured and fertilized wou...

Gatewood, L.C.; Ackerman, E.; Ewy, W.; Elveback, L.; Fox, J.P., 1971: Simulation of models of enteric virus epidemics. International Journal of Bio-Medical Computing 2(3): 201-213

Sakamoto T., 1990: Effect of egf on the preimplantation development of mouse embryos fertilized in vitro. The present study was undertaken to know whether EGF exerts beneficial effect on the preimplantation development of mouse embryos fertilized in vitro. The development of ICR embryos beyond the 2-cell stage at 120hr after insemination was significa...

Izquierdo J.A.; Jofre I.J.; Acevedo C., 1971: Seasonal variations of myo cardial catecholamines in the frog bufo arenarum effect of reserpine. Libro Homenaje Al Prof B Lorenzo-Velazquez (Book Dedicated to Prof B Lorenzo-Velazquez) 1160p Illus Editorial Oteo: Madrid, Spain 1041-1048

Repciuc, E., 1976: Canonical analysis as demonstrated on an example (proceedings). Gegenbaurs Morphologisches Jahrbuch 122(3): 397-403

Malhotra, V.; Kachroo, A.; Sapru, H.N., 1993: Role of alpha 1-adrenergic receptors in the intermediolateral column in mediating the pressor responses elicited by the stimulation of ventrolateral medullary pressor area. Microinjections of alpha 1-adrenergic receptor agonists into the intermediolateral cell column of the spinal cord (IML) elicit sympathoexcitatory responses. This observation, together with the identification of projections of epinephrine-containin...

Zimin Y.I., 1973: Increased emigration of cells from the thymus of rats in the initial period of stress reaction. Izvestiya Akademii Nauk SSSR Seriya Biologicheskaya (4): 517-524