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Cells Respond to Distinct Nanoparticle Properties with Multiple Strategies As Revealed by Single-Cell RNA-Seq


, : Cells Respond to Distinct Nanoparticle Properties with Multiple Strategies As Revealed by Single-Cell RNA-Seq. Acs Nano 10(11): 10173-10185

NlmCategory="UNASSIGNED">The impact of distinct nanoparticle (NP) properties on cellular response and ultimately human health is unclear. This gap is partially due to experimental difficulties in achieving uniform NP loads in the studied cells, creating heterogeneous populations with some cells "overloaded" while other cells are loaded with few or no NPs. Yet gene expression studies have been conducted in the population as a whole, identifying generic responses, while missing unique responses due to signal averaging across many cells, each carrying different loads. Here, we applied single-cell RNA-Seq to alveolar epithelial cells carrying defined loads of aminated or carboxylated quantum dots (QDs), showing higher or lower toxicity, respectively. Interestingly, cells carrying lower loads responded with multiple strategies, mostly with up-regulated processes, which were nonetheless coherent and unique to each QD type. In contrast, cells carrying higher loads responded more uniformly, with mostly down-regulated processes that were shared across QD types. Strategies unique to aminated QDs showed strong up-regulation of stress responses, coupled in some cases with regulation of cell cycle, protein synthesis, and organelle activities. In contrast, strategies unique to carboxylated QDs showed up-regulation of DNA repair and RNA activities and decreased regulation of cell division, coupled in some cases with up-regulation of stress responses and ATP-related functions. Together, our studies suggest scenarios where higher NP loads lock cells into uniform responses, mostly shutdown of cellular processes, whereas lower loads allow for unique responses to each NP type that are more diversified proactive defenses or repairs of the NP insults.

US$19.90

PMID: 27788331

DOI: 10.1021/acsnano.6b05452


Other references

Jansen; Freeman, 1986: Structural properties and electron density of NaCl. Physical Review. B, Condensed Matter 33(12): 8629-8631

Vigo, J.; Brau, R.H., 1992: Carotid endarterectomy in Puerto Rico. The experience of the authors performing carotid endarterectomy in Puerto Rico is reported. The study was stimulated by the recently published results of the Carotid Endarterectomy Cooperative Trial groups in North America and Europe. This series...

Dubrovskaia, V.F.; Gubareva, A.V., 1987: Protective effect of total gaseous hypoxia on the development of remote pathology of the lungs in rats exposed to a one-time local irradiation. A comparative study was conducted on the development of a remote radiation pathology in rat lungs after single local irradiation with doses of 10, 14.3 and 20 Gy in the air, and 14.3 and 20 Gy in a gas hypoxic mixture containing 10 per cent of oxy...

Boundy, T., 1983: Blood sampling in sheep. In Practice 5(3): 101-102

Morawik, I.; Jabłoński, Mław., 2017: Effect of Perioperative Bacteriological Safety on Hip Joint Infection after Hip Replacement. In terms of clinical importance, deep infections are the most severe and problematic complications of hip replacement, and limiting the risk of these complications continues to pose a medical and organisational challenge. The aim of this retrospec...

Eyles, J.E.; Sharp, G.J.E.; Williamson, E.D.; Spiers, I.D.; Alpar, H.O., 1997: Immune responses to mucosally administered co-encapsulated Yersinia pestis antigens. Journal of Pharmacy & Pharmacology 49(SUPPL 4): 85, Sept

Herbert, J.M.; Lalé, A.; Pereillo, J.M.; Derocq, J.M.; Casellas, P., 1996: Echinomycin suppresses the pyrogenic effects of endotoxin and interleukin-1 beta in human endothelial cells and peripheral blood mononuclear cells. Endotoxin (LPS) and interleukin-1 beta (IL-1 beta) increased in a dose-dependent manner the expression of tissue factor, an ubiquitous membrane-anchored glycoprotein that initiates blood coagulation at the surface of human umbilical vein endotheli...

Womer, K.L.; Huang, Y.; Herren, H.; Dibadj, K.; Peng, R.; Murawski, M.; Shraybman, R.; Patton, P.; Clare-Salzler, M.J.; Kaplan, B., 2010: Dendritic cell deficiency associated with development of BK viremia and nephropathy in renal transplant recipients. BK virus nephropathy (BKVN) is a significant cause of renal allograft loss. Although overall intensity of immunosuppression is the greatest risk factor, recipient immune factors likely also play a role in the pathogenesis. Dendritic cells (DC) are...

Scholz, K.H.; Werner, G.S.; Andreas, S.; Unterberg, C.; Buchwald, A.B., 1999: Treatment of deep venous thrombosis with temporary Vena cava filter placement, mechanical recanalization, and local thrombolysis guided by frequent fluoroscopic controls. Circulation 100(18 SUPPL ): I 172, Nov 2

Anonymous, 1998: Life in the fast lane. The anything-but-normal life of a GHA lobbyist. Gha Today 42(2): 1, 6-1, 8