geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Chloride concentrations in human hepatic cytosol and mitochondria are a function of age


, : Chloride concentrations in human hepatic cytosol and mitochondria are a function of age. Biochemical and Biophysical Research Communications 459(3): 463-468

We recently reported that, in a concentration-dependent manner, chloride protects hepatic glutathione transferase zeta 1 from inactivation by dichloroacetate, an investigational drug used in treating various acquired and congenital metabolic diseases. Despite the importance of chloride ions in normal physiology, and decades of study of chloride transport across membranes, the literature lacks information on chloride concentrations in animal tissues other than blood. In this study we measured chloride concentrations in human liver samples from male and female donors aged 1 day to 84 years (n = 97). Because glutathione transferase zeta 1 is present in cytosol and, to a lesser extent, in mitochondria, we measured chloride in these fractions by high-performance liquid chromatography analysis following conversion of the free chloride to pentafluorobenzylchloride. We found that chloride concentration decreased with age in hepatic cytosol but increased in liver mitochondria. In addition, chloride concentrations in cytosol, (105.2 ± 62.4 mM; range: 24.7-365.7 mM) were strikingly higher than those in mitochondria (4.2 ± 3.8 mM; range 0.9-22.2 mM). These results suggest a possible explanation for clinical observations seen in patients treated with dichloroacetate, whereby children metabolize the drug more rapidly than adults following repeated doses, and also provide information that may influence our understanding of normal liver physiology.

US$19.90

PMID: 25748576

DOI: 10.1016/j.bbrc.2015.02.128


Other references

Kaufman J.S.; Hamburger R.J.; Flamenbaum W., 1979: Tubulo glomerular feedback response during reduced renal perfusion pressure. Kidney International 16(6): 855

Gladstone, H.S., 1924: The distribution of the Black Grouse in Great Britain. British Birds, 18: 66-68

W.S.McGreal, 1979: Marine erosion of glacial sediments from a low-energy cliffline environment near Kilkeel, Northern Ireland. Cliffline erosion by marine processes along a low-energy, crenulated coastline near Kilkeel, is described. Observations over the period December 1973 to November 1975 and predictions based on these observations suggest a mean frequency of attack o...

Vergens, H.; Grozdea, J.; Corberand, J., 1984: Liver like and placenta like alkaline phosphatase ec 3.1.3.1 iso enzymes in normal human neutrophils. Alkaline phosphatase (AP) was characterized in the neutrophil polymorphonuclear leukocytes (PMN) from 14 healthy adults. The biochemical properties of the isoenzymes were analyzed by the following methods: enzyme assays, slab gel electrophoresis,...

Gonzalez, A., M.; Walther, D.; Pazos, A.; Uhl, G., R., 1994: Synaptic vesicular monoamine transporter expression: Distribution and pharmacologic profile. The human vesicular monoamine transporter (hSVMT) cDNA predicts a protein of 515 amino acids that shares 92% amino acid identity with the rat cDNA. Northern analyses reveal expression of 4.3 kb SVMT mRNAs in rat hypothalamus, midbrain and brainste...

Lazzerini Fabio Tadeu; Penteado Antonio Henrique Dantas da Gama, 2000: Ecotourism associated to sulphureous water in Sao Pedro and surrounding cuestas. International Geological Congress, Abstracts = Congres Geologique International, Resumes 31: (unpaginated

Latkowska-Starkiewiczowa, J., 1948: Contribution to the etiology of intracranial haemorrhage in newborn. Polski Tygodnik Lekarski 3(15): 464-467

B.J., 1926: Sir William Maddock Bayliss, 1866-1924. Proc Roy Soc B9: Xvii-Xxxii

Aderca I., 1978: Pitfalls in the sensitivity of hepatitis b surface antigen detection. Revue Roumaine De Medecine Virologie: 87-91

Wallet, F.; Pol, A.; Duthilly, A.; Roussel Delvallez, M.; Besson, C.; Courcol, R., 1999: Salmonella hadar pneumonia in a heart transplant patient. Clinical Microbiology Newsletter 21(16): 133-134, Aug 15