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Circulating microRNAs as biomarkers of adult Crohn's disease


, : Circulating microRNAs as biomarkers of adult Crohn's disease. European Journal of Gastroenterology & Hepatology 27(9): 1038-1044

Previous studies have found a differential expression of microRNAs (miRNAs) in the blood of patients with Crohn's disease (CD) compared with healthy controls. The aim of this study was to identify circulating miRNAs expressed in CD and assess their performance as biomarkers in patients with clinically suspected or known CD. The study consisted of two parts: (a) miRNA profiling: The miRNA expression pattern was examined in six patients with CD and six controls using OpenArray miRNA profiling, and the best miRNAs were selected according to their P-value. (b) Validation cohort: In a well-characterized cohort of 102 patients with suspected or known CD, miRNAs identified by miRNA profiling or selected from previously published studies (hsa-miR-16, hsa-miR-21, hsa-miR-106a, and hsa-miR-140-3p) were measured in plasma using reverse transcription PCR. miRNA profiling: hsa-miR-369-3p, hsa-miR-376a, hsa-miR-376, hsa-miR-411#, hsa-miR-411, and mmu-miR-379 were downregulated in CD patients compared with the controls; hsa-miR-200c, hsa-miR-181-2#, and hsa-miR-125a-5p were upregulated (P<0.05). Validation cohort: Only hsa-miR-16 was significantly downregulated in patients with CD compared with patients without CD (fold change 0.83, P=0.02). Receiver operating characteristic analyses showed an area under the curve of 0.65. miRNAs could not discriminate inflammatory from stricturing CD or small bowel CD from CD involving the colon. In a clinically relevant cohort of patients, miRNAs in plasma identified in the present and previous studies were inadequate biomarkers for the diagnosis of CD.

US$19.90

PMID: 26230660

DOI: 10.1097/MEG.0000000000000430


Other references

Giganti, E.D., 2001: Raising our voices to say who we are. Health Progress 81(6): 64-64

Crowell, S., 1991: Regression and replacement of hydranths in thecate hydroids, and the structure of hydrothecae. Hydrobiologia, 216-217: 69-73

Hernandez, P.; Navalesi, P.; Maltais, F.; Gursahaney, A.; Gottfried, S.B., 1994: Comparison of static and dynamic measurements of intrinsic PEEP in anesthetized cats. Dynamic measurements of intrinsic positive end-expiratory pressure (PEEPi,dyn) considerably underestimate values obtained under static conditions (PEEPi,stat) in patients with severe airway obstruction. This may be related to regional differences...

Harrer G., 1969: Clinical experimental experiences with a benzimidazolinone derivative clodazone. Cerletti, A (Editor) Excerpta Medica International Congress Series No 180 The Present Status Of Psychotropic Drugs Xi+ 572p Excerpta Medica Foundation: Amsterdam, Netherlands 508-511

Sirbu Z., 1962: Preliminary studies on the modifications of some histochemical elements in hog cholera pig enz alkaline phosphatase enz acid phosphatase enz oxidase enz lipase. Lucrarile Institutului de Cercetari Veterinare si Biopreparate Pasteur 1: 239-249

Kucheriuk, L.; Gareev, E., 1971: Fruit trees under dry conditions of culture. Biologiia Nekotorykh Drevesnykh Kustarnikovykh i Plodovykh Rastenii: 1-84

Toth,F.L.; Hizsnyik,E., 2008: Managing the inconceivable participatory assessments of impacts and responses to extreme climate change. A comprehensive understanding of the implications of extreme climate change requires an in-depth exploration of the perceptions and reactions of the affected stakeholder groups and the lay public. The project on "Atlantic sea level rise: Adap...

Seagle, R.L.; Cowgill, R.W., 1976: Fluorescence and the structure of proteins. XXII. Fluorescence of aminotyrosyl residues formed in ribonuclease A. 1. Tyrosyl residues on ribonuclease A were nitrated with tetranitromethane and then reduced to aminotyrosyl residues. By variation of reaction conditions and degree of exposure of tyrosyl residues it was possible to convert from 1 to all 6 tyrosyl...

Alvarenga, R.De, M., 1973: Problems of nature conservation, opinions and legislation. Brasil florestal: t 4 (15) 18-28

Karimi,A.; Dianatpour,R., 2008: Genotypic and phenotypic characterization of Echinococcus granulosus of Iran. Molecular and morphological analyses were used to study phenotypic and genotypic characteristics of two common sheep and camel strains of <i>Echinococcus granulosus</i>. Biometric analysis of fifteen characters showed that those 15 mor...