geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Co-delivery of siRNA and hypericin into cancer cells by hyaluronic acid modified PLGA-PEI nanoparticles


, : Co-delivery of siRNA and hypericin into cancer cells by hyaluronic acid modified PLGA-PEI nanoparticles. Drug Development and Industrial Pharmacy 42(5): 737-746

Malignant tumors cause more death because of the resistance of the hypoxic cancer cell toward radiotherapy. Targeting for hypoxic cancer area and gene silencing to overcome the hypoxia are two kinds of important therapeutic strategies for treating tumors. In order to explore the combined effects of gene therapy and hypericin (Hy) on tumor cells, hypoxia-inducible factor 1 alpha (HIF-1α) small interfering ribonucleic acid (siRNA) was transfected into the hypoxic human nasopharyngeal carcinoma (CNE2) cells using Hy-encapsulated nanocomplexes (Hy-HPP NPs) as a carrier which would achieve dual targeting to the tumor necrosis area. NPs were prepared by emulsion-diffusion-evaporation method. Formulations were evaluated by conducting in vitro physicochemical studies, electrophoresis, in vivo study, and biochemical studies. Hy-loaded nanoparticles with a mean size of around 160 nm was able to enhance the accumulation in the tumors by enhanced permeability and retention effect. The electrophoresis confirmed the good stability of siRNA/Hy-HPP NPs in the presence of phosphate-buffered saline (pH 7.4), competitive heparin, and RNase. The results of transfection showed that the uptake of siRNA was significantly increased up to 50% in CNE2 cells. The level of the HIF-1α with Hy-encapsulated nanocomplexes was significantly reduced to 30% in the transfected CNE2 cells. In vivo studies, the carrier exhibited higher intensity at the tumor tissue cells and higher affinity toward the necrotic tumor tissue. Results demonstrated that Hy-HPP NPs could significantly enhance the tranfection efficiency of siRNA, suggesting Hy-encapsulated nanoparticle as an efficient gene carrier. The co-delivery of HIF-1α siRNA (siHIF-1α) and Hy could efficiently decrease the level of HIF-1α and increase the affinity toward necrotic tissues. Hence, this is a promising strategy for further application in radiotherapy.

US$19.90

PMID: 26472259

DOI: 10.3109/03639045.2015.1091469


Other references

Li, X.; Kang, T.; Quan, H.; Liu, J.; Xu, J.; Tian, X., 2011: Study of exercise heart rate variability based on correlation dimension. This paper is mainly devoted to the relationship between the correlation dimension (CD) of exercise heart rate variability (EHRV) and the status of cardiovascular function. Hypertensive patients and healthy people were enrolled in two contrast gro...

López, R.; García, J.L.; Ronda, C.; García, E.; García, P., 1994: Lytic enzymes of the pneumococcal system. The cell wall lytic enzymes of Streptococcus pneumoniae and its bacteriophages provide a reliable experimental model to support the extended idea concerning the modular organization of most proteins. The comparative analysis of the deduced amino a...

Grivegnee, A.; Delince, P.; Ectors, P., 1981: Comparative aspects of occult intrasacral meningocele with conventional X-ray, myelography and CT. A case of occult intrasacral meningocele is reported and the diagnostic reliability of conventional roentgenography, myelography and CT for the management of this rare lesions are evaluated. Probably, CT with the use of an intrathecal contrast age...

Meyers, F.; Evans, J.M., 1964: The Serum Transaminase (S-Got) And Electrocardiogram In Autopsy-Confirmed Acute Myocardial Infarction. American Heart Journal 67: 15-17

Hinshaw, W.R., 1943: Diseases of turkeys. This bulletin gives data on turkey diseases published from the California Agricultural Experiment Station and other sources. It is divided into sections as follows: -principles of disease prevention, dietary diseases; fungous diseases; bacterial a...

Chakrabarty, T.; Balakrishnan, N.P.; Gupta, D., 1997: Trichomes in Indian species of Croton L. (Euphorbiaceae) and their systematic applications. Comparative studies on trichomes of 29 species of Croton L. (Euphorbiaceae) occurring in India and adjoining countries, were carried out. The trichomes in Croton are of great diagnostic value and may be employed for species grouping or even identi...

Waterman, J.A.; Middlebrook, G., 1957: The binding of various long-chain fatty acids by serum albumin. Clinica Chimica Acta; International Journal of Clinical Chemistry 2(5): 449-460

Morrison, R.G., 1986: Medical and public health aspects of boxing. In recent years, an extensive medical literature has accumulated regarding the health hazards associated with boxing. As the evidence that boxing produces irreversible brain damage in boxers has become more compelling, physicians have begun to con...

Milunsky, J.M.; Skare, J.C.; Milunsky, A., 1991: Presymptomatic and prenatal diagnosis of myotonic muscular dystrophy with linked DNA probes. Advances in neurogenetics are facilitating clinical care. Localization of the mutant gene that causes myotonic muscular dystrophy (DM) to chromosome 19 enabled our predictive testing using linked DNA probes in 74 members of 12 families at risk. In...

Pashkow, F.J., 2000: In preventive cardiology there is no 'one true thing'. Prescribing exercise, dietary changes, or stress management regimens separately and in the short term will ultimately fail to prevent coronary events or bring about regression of existing coronary disease. Only a multifactorial approach that focus...